A5026444785- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Brucella: diagnosis, epidemiology, treatment
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Tuberculosis Research and Epidemiology
- Genetic and phenotypic traits in livestock
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Immune Response and Inflammation
- Veterinary medicine and infectious diseases
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Blood groups and transfusion
- Antimicrobial Resistance in Staphylococcus
- Diet and metabolism studies
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- RNA and protein synthesis mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Antimicrobial Peptides and Activities
University of Naples Federico II
2010-2023
Institute of Immunology
2006
National Agency for New Technologies, Energy and Sustainable Economic Development
1997
University of Calabria
1977-1978
Animal Production Institute
1978
The present study describes a bacteriophage (M(Sa)) active against Staphylococcus aureus, including methicillin-resistant staphylococcal strains. When inoculated into mice simultaneously with S. aureus A170 (10(8) CFU/mouse), phage (10(9) PFU) rescued 97% of the mice; when applied to nonlethal (5 x 10(6) CFU/mouse) 10-day infections, also fully cleared bacteria. M(Sa), delivered inside macrophages by kills intracellular staphylococci in vivo and vitro. can prevent abscess formation reduce...
The most serious criticisms leveled at bacteriophage therapy are as follows: phages induce neutralizing antibodies, active only when administered shortly after bacterial infection, and phage-resistant bacteria emerge rapidly in the course of therapy.Phages lytic for several Salmonella enterica serovars were isolated by means standard protocols from feces patients with gastroenteritis. Growth S. serovar Paratyphi B (Salp572(phi1S)) presence phage phi1 (selected among 8 its larger host range)...
In the presence of a bacteriophage (a bacteria-attacking virus) resistance is clearly beneficial to bacteria. As expected in such conditions, resistant bacteria emerge rapidly. However, absence phage, often display reduced fitness, compared their sensitive counterparts. The present study explored fitness cost associated with phage-resistance as an opportunity isolate attenuated strain S. aureus. phage-resistant A172 was isolated from phage-sensitive A170 MSa phage. Acquisition altered...
ABSTRACT Brucellosis is a costly disease of water buffaloes ( Bubalus bubalis ). Latent infections and prolonged incubation the pathogen limit efficacy programs based on eradication infected animals. We exploited genetic selection for resistance as an approach to control buffalo brucellosis. tested 231 cows presence anti- Brucella abortus antibodies (by agglutination complement fixation tests) Nramp1 genotype PCR-denaturing gradient gel electrophoresis). When animals (58 cases 173 controls)...
ABSTRACT Antistaphylococcal activity of the novel chimeric endolysin PRF-119 was evaluated with microdilution method. The MIC 50 and 90 398 methicillin-susceptible Staphylococcus aureus isolates were 0.098 μg/ml 0.391 μg/ml, respectively (range, 0.024 to 0.780 μg/ml). Both values 776 methicillin-resistant S. 1.563 All 192 clinical coagulase-negative staphylococci exhibited >50 μg/ml. In conclusion, very good specifically against .
Abstract There is epidemiological evidence that H. pylori might predispose to Alzheimer’s disease. To understand the cellular processes potentially linking such unrelated events, we incubated human gastric cells MNK-28 with peptide Hp(2-20). We then monitored activated genes by global gene expression. The modulated 77 genes, of which 65 are listed in AlzBase database and include hallmarks disease: APP, APOE, PSEN1, PSEN2. A large fraction (30 out 77) belong inflammation pathway. Remarkably,...
Temporins are antimicrobial peptides secreted by the granular glands of European red frog (Rana temporaria). They 10–14 amino acid long polypeptides active prevalently against gram positive bacteria. This study shows that a synthetic temporin B analogue (TB-YK), acquires capacity to act in synergism with A and exert anti-inflammatory activity vivo negative Administration 3.4 mg/Kg (TA)+1.6 TB-YK, given individual mice concurrently lethal dose bacteria (gram or negative), rescued 100%...
Mannose-binding lectin (MBL) activates the complement system in an antibody-independent manner, enhances complement-mediated phagocytosis, and plays a major role regulation of inflammatory cytokine release by monocytes.Case patients (277 with pulmonary tuberculosis) control subjects (288 household contacts) were tested polymerase chain reaction (PCR) for polymorphisms at promoter exon 1 regions MBL gene. Diagnosis tuberculosis, based on findings from chest radiography sputum smear...
Journal Article Heterogeneous shedding of Brucella abortus in milk and its effect on the control animal brucellosis Get access R. Capparelli, Capparelli Faculty Biotechnological Sciences, University Naples "Federico II", Naples, Italy Search for other works by this author on: Oxford Academic Google Scholar M. Parlato, Parlato Agriculture, Iannaccone, Iannaccone S. Roperto, Roperto Veterinary Medicine, Marabelli, Marabelli Department Public Health, Nutrition Food Safety, Ministry Rome, F. D....
ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti- Brucella abortus antibodies (by skin test, agglutination complement fixation test) Nramp1 genotype capillary electrophoresis). Four alleles ( Nramp1A , - B C D ) were detected in 3′ untranslated region gene. The BB was represented among only controls, providing evidence that this confers resistance to . monocytes from (resistant) subjects displayed a higher basal level mRNA lower number viable...
Helicobacter pylori (Hp) is a Gram-negative bacterium colonizing the human stomach. Nuclear Magnetic Resonance (NMR) analysis of intracellular gastric carcinoma cells (MKN-28) incubated with Hp cell filtrate (Hpcf) displays high levels amino acids, including branched chain acids (BCAA) isoleucine, leucine, and valine. Polymerase reaction (PCR) Array Technology shows upregulation mammalian Target Of Rapamycin Complex 1 (mTORC1), inflammation, mitochondrial dysfunction. The review literature...
Staphylococcus aureus, in spite of antibiotics, is still a major human pathogen causing wide range infections. The present study describes the new vaccine A170PG, peptidoglycan-based vaccine. In mouse model infection, A170PG protects mice against lethal dose S. aureus. Protection lasts at least 40 weeks and correlates with increased survival reduced colonization. extends into drug-resistant (MRSA or VISA) genetically diverse clinical strains. effective when administered - single without...
Bacterial biofilm provides bacteria with resistance and protection against conventional antimicrobial agents the host immune system. Bacteriophages are known to move across make it permeable antimicrobials. Mineral hydroxyapatite (HA) can improve lytic activity of bacteriophages, and, together eicosanoic acid (C20:0), destroy structure. Here, we demonstrate efficacy combined use phage, HA C20:0 Xanthomonascampestris pv campestris (Xcc) biofilm. We used nuclear magnetic resonance (NMR)-based...
Abstract The Toll-interleukin 1 receptor superfamily includes the genes interleukin receptor-like ( IL1RL1 ), Toll like receptors TLRs myeloid differentiation primary-response 88 MyD88 and adaptor-like TIRAP ). This study describes interaction between MyD88, against Helicobacter pylori infection. Cases controls were genotyped at polymorphic sites rs6853, rs8177374 rs11123923. results show that specific combinations of - (AA-CT; P: 2,8 × 10 –17 ) (AA-CT-AA; 1,4 –8 – but not alone—act...
The nuclear DNA content of seven mouse laboratory strains has been measured by flow cytometry. differences observed between as well those sexes within the strain were all statistically significant. highest (approximately 6.4 pg/female nucleus) was found in Balb/c strain; lowest 5.7 pg/male C3H/he strain. difference varied from 1.6% (in CD-1 mice) to 6.3% nude mice). interest these results is twofold. First, can now be used study adaptive significance genome size variation, so far studied...