A5091717095- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- Proteoglycans and glycosaminoglycans research
- Bacterial biofilms and quorum sensing
- Carbohydrate Chemistry and Synthesis
- Antibiotic Resistance in Bacteria
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- Microbial Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Microbial infections and disease research
- Galectins and Cancer Biology
- Probiotics and Fermented Foods
- Virus-based gene therapy research
- Antifungal resistance and susceptibility
- Viral gastroenteritis research and epidemiology
Jiaxing University
2025
Manchester Academic Health Science Centre
2018-2024
University of Manchester
2014-2024
Chongqing University
2021-2023
University of Tübingen
2008-2018
German Center for Infection Research
2013-2018
Shanghai Jiao Tong University
2017
Institute of Medical Microbiology and Hygiene
2009
Research Center Borstel - Leibniz Lung Center
2009
Otto-von-Guericke University Magdeburg
2008
Staphylococcus aureus peptidoglycan (PG) is densely functionalized with anionic polymers called wall teichoic acids (WTAs). These contain three tailoring modifications: d-alanylation, α-O-GlcNAcylation, and β-O-GlcNAcylation. Here we describe the discovery biochemical characterization of a unique glycosyltransferase, TarS, that attaches β-O-GlcNAc (β-O-N-acetyl-D-glucosamine) residues to S. WTAs. We report methicillin resistant (MRSA) sensitized β-lactams upon tarS deletion. Unlike strains...
The molecular interactions between staphylococcal phages and host cell surfaces are poorly understood. Employing Staphylococcus aureus teichoic acid mutants, we demonstrate that wall (WTA), but not lipoteichoic acid, serves as a receptor for siphovirus myovirus, while only the requires glycosylated WTA.
Mobile genetic elements (MGEs) encoding virulence and resistance genes are widespread in bacterial pathogens, but it has remained unclear how they occasionally jump to new host species. Staphylococcus aureus clones exchange MGEs such as S. pathogenicity islands (SaPIs) with high frequency via helper phages. Here we report that the ST395 lineage is refractory horizontal gene transfer (HGT) typical exchanges SaPIs other species genera including epidermidis Listeria monocytogenes. produces an...
Wall teichoic acid (WTA) glycopolymers are major constituents of cell envelopes in Staphylococcus aureus and related Gram-positive bacteria with important roles wall maintenance, susceptibility to antimicrobial molecules, biofilm formation, host interaction. Most S. strains express polyribitol phosphate WTA substituted d-alanine N-acetylglucosamine (GlcNAc). sugar modifications highly variable have been implicated bacteriophage immunogenicity, but the pathway enzymes staphylococcal...
Heparan sulfate 3-<i>O</i>-sulfotransferase transfers to the 3-OH position of a glucosamine residue heparan (HS) form 3-<i>O</i>-sulfated HS. The contributes two important biological functions HS: binding antithrombin and thereby carrying anticoagulant activity, herpes simplex viral envelope glycoprotein D serve as an entry receptor for virus 1. A total five HS isoforms were reported previously. Here we report isolation characterization novel isoform, designated isoform 5 (3-OST-5). 3-OST-5...
Wall teichoic acid (WTA) or related polyanionic cell wall glycopolymers are produced by most Gram-positive bacterial species and have been implicated in various cellular functions. WTA the proton gradient across membranes known to control activity of autolysins but molecular details these interactions poorly understood. We demonstrate that contributes substantially proton-binding capacity Staphylococcus aureus walls controls autolysis largely via major autolysin AtlA whose is decline at...
Many Staphylococcus aureus have lost a major genetic barrier against phage infection, termed clustered regularly interspaced palindromic repeats (CRISPR/cas). Hence, S. strains frequently exchange material via phage-mediated horizontal gene transfer events, but, in turn, are vulnerable particular to lytic phages. Here, novel strategy of is described, which protects the activity Podoviridae, unique family staphylococcal phages with short, non-contractile tails. Unlike most phages, Podoviridae...
Despite the importance of phages in driving horizontal gene transfer (HGT) among pathogenic bacteria, underlying molecular mechanisms mediating phage adsorption to S. aureus are still unclear. Phage ϕ11 is a siphovirus with high transducing efficiency. Here, we show that tail protein Gp45 localized within baseplate. was efficiently neutralized by anti-Gp45 serum, and its host cells inhibited recombinant dose-dependent manner. Flow cytometry analysis demonstrated biotin-labelled stained...
Heparan sulphate (HS) 3-O-sulphotransferase transfers to the 3-OH position of glucosamine residue HS form 3-O-sulphated HS. The modified by isoform 3 binds HSV-1 (herpes simplex virus type 1) gD (envelope glycoprotein D), and resultant serves as an entry receptor for HSV-1. In present paper, we report isolation characterization a novel isoform, designated 6 (3-OST-6). Mouse 3-OST-6 gene was identified in EST (expressed sequence tag) database cloned into pcDNA3.1/Myc-His vector. A CHO...
We have identified and characterized an N-acetylgalactosamine-4-O-sulfotransferase designated dermatan-4-sulfotransferase-1 (D4ST-1) (GenBank(TM) accession number AF401222) based on its homology to HNK-1 sulfotransferase. The cDNA predicts open reading frame encoding a type II membrane protein of 376 amino acids with 43-amino acid cytoplasmic domain 316-amino luminal containing two potential N-linked glycosylation sites. D4ST-1 has significant identity sulfotransferase (21.4%), 1...
Serum antibodies and mannose-binding lectin (MBL) are important host defense factors for adaptive innate immunity, respectively. Antibodies MBL also initiate the classical complement pathways, respectively, leading to opsonophagocytosis. We have shown previously that Staphylococcus aureus wall teichoic acid (WTA), a cell glycopolymer consisting of ribitol phosphate substituted with α- or β-O-N-acetyl-d-glucosamine (GlcNAc) d-alanine, is recognized by serum anti-WTA IgG. However, exact...
ABSTRACT The major clonal lineages of the human pathogen Staphylococcus aureus produce cell wall-anchored anionic poly-ribitol-phosphate (RboP) wall teichoic acids (WTA) substituted with d -Alanine and N -acetyl- -glucosamine. phylogenetically isolated S. ST395 lineage has recently been found to a unique poly-glycerol-phosphate (GroP) WTA glycosylated -galactosamine (GalNAc). clones bear putative biosynthesis genes on novel genetic element probably acquired from coagulase-negative...
Biofilms are major contributors to delayed wound healing and there is a need for clinically relevant experimental models assess theranostics. Microorganisms release volatile organic compounds (VOCs) the ability identify these in infected cutaneous wounds could lead efficient non-invasive diagnosis. The aims here were develop bacterial biofilm formation their VOC profiles an vitro model validate human ex vivo incisional excisional models. Biofilm development was assessed using multiple...
Abstract Phages play key roles in the pathogenicity and adaptation of human pathogen Staphylococcus aureus . However, little is known about molecular recognition events that mediate phage adsorption to surface S. The lysogenic siphophage ϕ11 infects SA113. It was shown previously requires α- or β-N-acetylglucosamine (GlcNAc) moieties on cell wall teichoic acid (WTA) for adsorption. Gp45 identified as receptor binding protein (RBP) involved this process GlcNAc residues WTA were found be...
Chitinases are produced throughout the growth process of fungi and thought to play important roles in morphogenesis. Aspergillus fumigatus , is an pathogen immunocompromised individuals which it causes pneumonia invasive disseminated disease with high mortality; also known produce chitinase. We have induced exceptionally stable extracellular chitinase A. YJ‐407, could be isolated readily a homogeneous form by using ammonium sulfate precipitation followed DEAE‐cellulose chromatography...
We have identified and characterized anN-acetylgalactosamine-4-O-sulfotransferase designated chondroitin-4-sulfotransferase-3 (C4ST-3) (GenBankTM accession number AY120869) based on its homology to HNK-1 sulfotransferase (HNK-1 ST). The cDNA predicts an open reading frame encoding a type II membrane protein of 341 amino acids with 12-amino acid cytoplasmic domain 311-amino luminal containing single potential N-linked glycosylation site. C4ST-3 has the greatest sequence identity when aligned...
ABSTRACT Antistaphylococcal activity of the novel chimeric endolysin PRF-119 was evaluated with microdilution method. The MIC 50 and 90 398 methicillin-susceptible Staphylococcus aureus isolates were 0.098 μg/ml 0.391 μg/ml, respectively (range, 0.024 to 0.780 μg/ml). Both values 776 methicillin-resistant S. 1.563 All 192 clinical coagulase-negative staphylococci exhibited >50 μg/ml. In conclusion, very good specifically against .
Staphylococcus aureus is exposed to multiple antimicrobial compounds, including oxidative burst products and antibiotics. The various mechanisms regulatory pathways governing susceptibility or resistance are complex only superficially understood. Bacillus subtilis recently has been shown control disulfide stress responses by the thioredoxin-related YjbH protein, which binds transcriptional regulator Spx controls its degradation via proteasome-like ClpXP protease. We show that S. homolog a...
Most bacterial glycoproteins identified to date are virulence factors of pathogenic bacteria, i.e. adhesins and invasins. However, the impact protein glycosylation on major human pathogen Staphylococcus aureus remains incompletely understood. To study in staphylococci, we analyzed lysostaphin lysates methicillin-resistant (MRSA) strains by SDS-PAGE subsequent periodic acid-Schiff's staining. We detected four (>300, ∼250, ∼165, ∼120 kDa) two (>300 ∼175 glycosylated surface proteins with...
Staphylococcus aureus is a common skin commensal but also associated with various and soft tissue pathologies. Upon invasion, S. detected by resident innate immune cells through pattern-recognition receptors (PRRs), although comprehensive understanding of the specific molecular interactions lacking. Recently, we demonstrated that PRR langerin (CD207) on epidermal Langerhans senses conserved β-1,4-linked N-acetylglucosamine (GlcNAc) modification wall teichoic acid (WTA), thereby increasing...