This article looks at the discourses of Instagram users about interrupting use social or digital media, through hashtags such as “socialmediadetox,” “offline,” “disconnecttoreconnect.” We identified three predominant themes: posts announcing recounting voluntary interruption, mostly a positive experience associated to regaining control over time, relationships, and their own well-being; others actively campaigning for this type disconnection, attempting convert others; disconnection...

Sharenting (sharing parenting on social media) has become a widespread activity, and some of those parents family influencers. Female influencers have been the rise, partly as an alternative to precariousness job market. This article presents qualitative study 11 Portuguese mummy influencers, analysing media content observed throughout 2.5 years, well discourses them. It focuses how these female creators portray family, work–life balance influencer their boundaries for privacy intimacy....

Digital nomads (DNs) are independent professionals who rely heavily on digital media and communications, not bound to the constraints imposed by traditional organizations, prioritize variables such as cheaper living costs when deciding where live. Communication technologies enable emergence of this lifestyle that is characterized high mobility, self-efficacy, location-independent resource-sharing, minimalism. The immediate outcome Covid-19 pandemic created an unprecedented space for debate...

For decades, cells of the Gram-positive bacterial pathogen

This paper analyzes how female teen YouTubers manage intimacy and trust in the narration of their personal lives ways which this is perceived by followers. The analysis two case studies popular from Portugal Brazil – SofiaBBeauty Manoela Antelo, respectively revealed that presentation anchored discourses about whom they are with where are, what doing, tastes styles. Through comments on videos, followers express connections vloggers, based a sense proximity, desire for exclusivity...

Summary The Gram‐positive pathogen Staphylococcus aureus divides by synthesizing the septum in three orthogonal planes over consecutive division cycles. This process has to be tightly coordinated with chromosome segregation avoid bisection of nucleoid septum. Here we show that deletion occlusion effector Noc S. results formation Z‐rings nucleoid, as well DNA breaks, indicating an important role antiguillotine checkpoint prevents septa from forming DNA. Furthermore, deleted cells multiple...

EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved the coordination between cell growth and division control elongation-division cycle. We have now studied role Staphylococcus aureus homologue B. subtilis protein shown that it not essential for viability. conditional null mutants an overall increase average size, compared to wild type strains. In larger ezrA mutant S. cells, wall synthesizing Penicillin Binding Proteins (PBPs) are properly localized. This suggests there may...

Sharenting, or the practice of sharing one’s parenting information about children on social media, occurs in an increasingly platformized digital culture, where visual formats are central across participatory and commercial repositories. This paper investigates articulation between sharenting as performed by celebrities wider construction children’s identities. Through qualitative content analysis, this research looks at how Cristiano Ronaldo, most-followed individual Instagram since 2018,...

The polymerization of peptidoglycan is the result two types enzymatic activities: transglycosylation, formation linear glycan chains, and transpeptidation, peptide cross-bridges between strands. Staphylococcus aureus has four penicillin binding proteins (PBP1 to PBP4) with transpeptidation activity, one which, PBP2, a bifunctional enzyme that also capable catalyzing transglycosylation reactions. Additionally, monofunctional transglycosylases have been reported in S. aureus: MGT, which shown...

Post-secular pilgrimage is increasingly popular, affording communitas (Maddrell & della Dora, 2013) as well collective effervescence (Serazio, to pilgrims. Pilgrimage can be augmented by digital media 2013), but pilgrims usually limit their access habitual devices, people, and services during (Jorge, 2023). This paper focuses on the case of World Youth Day 2023, hosted in Lisbon, seeks explore role social sustaining pilgrim communities atmospheres developed through time flows (Hitchen,...

This article considers how children’s memeability is entangled with commercial sharenting narratives through two case studies of (mothers) influencers and their daughters in Brazil Portugal. The Brazilian mother privileges cute aesthetics by enchantment an inspirational does not promote the child’s memeability. In contrast, Portuguese influencer cringe aesthetics, encouraging her daughter’s exploring ambivalence parenting humor a transgressive sharenting. findings point to unpredictability...

We have constructed a set of plasmids that allow expression, from their native chromosomal loci, Staphylococcus aureus proteins fused to one four different fluorescent (green protein [GFP], cyan [CFP], yellow [YFP], and mCherry), using two resistance markers (kanamycin erythromycin). also plasmid allows expression the ectopic spa locus in S. chromosome. This toolbox can be used for studies localization aureus, prominent pathogen both health care community settings.

Staphylococcus aureus is exposed to multiple antimicrobial compounds, including oxidative burst products and antibiotics. The various mechanisms regulatory pathways governing susceptibility or resistance are complex only superficially understood. Bacillus subtilis recently has been shown control disulfide stress responses by the thioredoxin-related YjbH protein, which binds transcriptional regulator Spx controls its degradation via proteasome-like ClpXP protease. We show that S. homolog a...

The field of microcelebrity is increasingly monetised, professionalised, and institutionalised, with the growing recognition content creators as social media influencers. This article looks at integration branded within youth digital culture, where participatory possibilities for self-expression are more entwined consumer culture. It seeks to discuss how producers understand brand culture audiences negotiate meanings commercialism inserted in their content. We look case SofiaBBeauty, a...

In a contemporary era, strongly characterized by digital omnipresence, celebrities share, via their social media accounts, experiences related to parenthood or, in other words, adopt sharenting practices. This article focuses on the visual and textual representation of children six Portuguese female male active Instagram 2020. The study aimed explore whether narratives content strategies conveyed about are integrated into authentic advertising content, how these vary according celebrity’s...

Young people struggle with permanent online connection that is associated their generation. This article looks at teenagers’ affective relationship to connectivity and disconnectivity, how it socioculturally influenced by the media, family, peers. It reports on an interview study 36 teenagers between 15 19 years of age from Norway Portugal. Our findings evidenced disconnection may arise out a latent feeling “disaffect” generated in experience ambience connected platform culture as well...

This paper draws upon a qualitative, exploratory study in Austria, Portugal, Denmark and England to argue that the role of digitally mediated ties parents’ social networks is significantly shaped by offline contexts, crucible moments transitional events parenting journeys. The qualitative interviews conducted with parents across 16 families, four each nation, draw out how abilities participate in, benefit from, contribute online – amidst an array groups, forums chat groups often restrained...

Prior research has pointed to cross-national variations in media attention for online risks, which are then mirrored parental concerns regarding the internet. However, little is known so far about how discursive environment around opportunities and risks of internet children shapes very context children’s own perceptions developed their experiences situated. The aim this contribution threefold: (1) understand what extent incorporate representations, worries discourses circulating among...

The needs and desires to disconnect, detox, log out have been turned into commodities found their expressions in detox camps, self-help books, “offline” branded apparel. Disconnection studies challenged the power of commodified disconnective practices create real social change. In this article, we build on notion affective attunement explore how disconnection provide differential ways for individuals respond challenges connectivity, they can form larger patterns resistance that cannot be...

This study consisted of a content analysis parenting portrayals in the 40 most popular Portuguese male and female producers on YouTube Instagram, sample published 2019. Female creators give disproportionately greater attention to are ones depicting everyday labor related it, whereas show themselves as fathers happy fun moments. By way their popularity visibility social media platforms, supported by platforms advertising realms, celebrities influencers amplifying traditional division through...

This article aims at understanding the construction of online risk and safety among children addressing lack research on children’s discourses their internet experiences. The analysis compares perceptions Portuguese (9-16) based an open-ended question about from EU Kids Online survey two questions a disadvantaged children. Theoretically, combines constructionist perspectives with framework matrix used in project. Methodological tools cognitive sciences critical discourse were to explore...

Social media influencers (SMI) are increasingly becoming the target of online aggression. The first part this study focuses on how group users deals with aggression and more specifically which coping strategies they use. In contrast to ordinary social users, SMI experience situations two levels: a personal level human brand level. As second aim, wants explore whether emotions one hand concerns other explain SMI's choice based combination Transaction Coping Theory Conservation Resources...

Digital presence and participation are often imagined as essential for contemporary feminist practices identities. However, engagements with digital social media platforms can be tempered by drawbacks tensions. This leads activists everyday feminists to the need negotiate dis/connection in their lives. article explores affective dimensions of contexts, grounding it on 22 in-depth interviews people engaged online feminisms activisms Portuguese context. The foregrounds relationship between...