A5014608486- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Wheat and Barley Genetics and Pathology
- Probiotics and Fermented Foods
- Antifungal resistance and susceptibility
- Sports Analytics and Performance
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Plant Taxonomy and Phylogenetics
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Microbial infections and disease research
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Plant Diversity and Evolution
- Aquaculture disease management and microbiota
- Plant Reproductive Biology
German Center for Infection Research
2021-2024
University of Tübingen
2015-2024
Northwest A&F University
2021-2024
University of California, San Diego
2023-2024
Huazhong University of Science and Technology
2024
Shanghai Children's Hospital
2023
Lanzhou University
2018-2022
Tropical Crops Genetic Resources Institute
2022
Beijing Forestry University
2011-2022
Nanjing Agricultural University
2022
Natural polymer Bombyx mori silk fibroin is used as a biotemplate to produce silver nanoparticles in situ under light (both incandescent and sunlight) at room temperature. Silk provides multiple functions the whole reaction system, serving reducing agent of silver, dispersing stabilizing resulted nanoparticles. As needs not any other chemicals only uses power source, synthetic route reported here rather environment-friendly energy-saving. The fibroin-silver nanoparticle composite prepared by...
Background Staphylococcus epidermidis is a common cause of nosocomial infections worldwide. This study analyzed the differences in genetic endowment and clonal lineages with pathogenesis resistance traits S. isolates collected from community hospital environments (patients healthcare staff) same ecological niche, time period, geographical location China. Methodology/Principal Findings Molecular epidemiology population analysis showed that nasal colonization rates Shanghai area China...
The failure of the Staphylococcus aureus (SA) IsdB vaccine trial can be explained by recall non-protective immune imprints from prior SA exposure. Here, we investigate natural human humoral to understand their broader impact on immunizations. We show that antibody responses against cell-wall-associated antigens (CWAs) are non-opsonic, while antibodies toxins neutralizing. Importantly, protective characteristics accurately predict corresponding vaccines CWAs and support vaccination toxins. In...
Many Staphylococcus aureus have lost a major genetic barrier against phage infection, termed clustered regularly interspaced palindromic repeats (CRISPR/cas). Hence, S. strains frequently exchange material via phage-mediated horizontal gene transfer events, but, in turn, are vulnerable particular to lytic phages. Here, novel strategy of is described, which protects the activity Podoviridae, unique family staphylococcal phages with short, non-contractile tails. Unlike most phages, Podoviridae...
Abstract Genome size varies greatly across the flowering plants and has played an important role in shaping their evolution. It been reported that many factors correlate with variation genome size, but few studies have systematically explored this at genomic level. Here, we scan information for 74 species from families 38 orders covering major groups of angiosperms (the taxonomic was acquired latest Angiosperm Phylogeny Group (APG IV) system) to evaluate correlation between different...
Pathogenic bacterial persisters are responsible for the recalcitrance of chronic and persistent infections to antimicrobial therapy. Although mechanisms persister formation survival have been widely studied in Escherichia coli, persistence Staphylococcus aureus remain largely unknown. Here, we screened a transposon mutant library clinical methicillin-resistant aureus(MRSA)strain, USA500 (ST8), under antibiotic pressure identified 13 genes whose insertion mutations resulted defect...
Abstract Alfalfa ( Medicago sativa L.) is one of the most important and widely cultivated forage crops. It commonly used as a vegetable medicinal herb because its excellent nutritional quality significant economic value. Based on Illumina, Nanopore Hi-C data, we assembled chromosome-scale assembly spp . caerulea (voucher PI464715), direct diploid progenitor autotetraploid alfalfa. The genome comprises 793.2 Mb genomic sequence 47,202 annotated protein-coding genes. contig N50 length 3.86 Mb....
Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that notable for its ability to form biofilm and high rates of antibiotic resistance. It serves as reservoir multiple antimicrobial resistance genes spread among the staphylococcal population by horizontal gene transfer such transduction. While phage-mediated transduction well studied in aureus, S. transducing phages have not been described detail yet. Here, we report characteristics four phages, 27,...
Abstract Structural variants (SVs) represent an important genetic resource for both natural and artificial selection. Here we present a chromosome-scale reference genome domestic yak (Bos grunniens) that has longer contigs scaffolds (N50 44.72 114.39 Mb, respectively) than reported any other ruminant genome. We further obtained long-read resequencing data 6 wild 23 yaks constructed SV map of 372,220 SVs covers the geographic range yaks. The majority contains repetitive sequences several are...
Numerous problems in many fields can be solved effectively through the approach of modeling by complex network analysis. Finding key nodes is one most important and challenging In previous studies, methods have been proposed to identify nodes. However, they rely mainly on a limited field local information, lack large-scale access global are also usually NP-hard. this paper, novel entropy mutual information-based centrality (EMI) proposed, which attempts capture far wider range greater...
Phellinus gilvus (Schwein.) Pat, a species of 'Sanghuang', has been well-documented for various medicinal uses, but the genome information and active constituents are largely unknown. Here, we sequenced whole-genome P. gilvus, identified phenylpropanoids as its key anti-cancer components, deduced their biosynthesis pathways. A 41.11-Mb sequence was assembled heatmap created with high-throughput chromosome conformation capture techniques data suggested all bins could be clearly divided into...
Abstract Acne is a dermatologic disease with strong pathologic association human commensal Cutibacterium acnes . Conspicuously, certain C. phylotypes are associated acne, whereas others healthy skin. Here we investigate if the evolution of enzyme contributes to health or acne. Two hyaluronidase variants exclusively expressed by strains, HylA and HylB, demonstrate remarkable clinical correlation acne health. We show that strongly pro-inflammatory, HylB modestly anti-inflammatory in murine...
Staphylococcus aureus, including meticillin-sensitive and -resistant S. aureus (MSSA MRSA, respectively), is associated with severe nosocomial human infections. This study aimed to investigate the molecular profile, dynamic changes genotype/phenotype correlation, of isolates recovered from different clinical specimens inpatients infection over a 6-year span at teaching hospital in Shanghai, China. Between 2005 2010, random sample 610 unique was collected samples for antibiotic susceptibility...
Abstract Medicago ruthenica has been recently cultivated as a new forage crop and recognized source of genes to improve abiotic stress tolerance in alfalfa because its remarkable drought, salinity‐alkalinity, cold snowy winters. Here, we reveal chromosome‐scale genome sequence M. based on Illumina, PacBio, Hi‐C data. The assembled consists 903.56 Mb with 50,268 annotated protein‐coding genes, which is larger contains relatively more than truncatula (420 44,623 genes) sativa spp. caerulea...
The pathobiont Staphylococcus aureus (Sa) induces nonprotective antibody imprints that underlie ineffective staphylococcal vaccination. However, the mechanism by which Sa modifies activity is not clear. Herein, we demonstrate IL-10 decisive factor abrogates protection in mice. Sa-induced B10 cells drive antigen-specific vaccine suppression affects both recalled and de novo developed B cells. Released promotes STAT3 binding upstream of gene encoding sialyltransferase ST3gal4 increases its...
To control the spread of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals, infection measures such as hand hygiene practices were introduced into teaching hospitals Shanghai, China, 2008. Currently, there is limited information characterizing latest hospital-acquired S. infections this area. Therefore, we sought to determine prevalence, molecular characteristics, and genotype-phenotype correlation Huashan Hospital, one largest Shanghai. Among 608 clinical isolates obtained...
Staphylococcus epidermidis is a common member of the human skin and nose microbiomes frequent cause invasive infections. Transducing phages accomplish horizontal transfer resistance virulence genes by mispackaging mobile-genetic elements, contributing to severe, therapy-refractory S. Lytic on other hand can be interesting candidates for new anti-S. phage therapies. Despite importance phages, we are only beginning unravel interactions. Recent studies shed light diversity, host range, receptor...
Abstract Pathobionts have evolved many strategies to coexist with the host, but how immune evasion mechanisms contribute difficulty of developing vaccines against pathobionts is unclear. Meanwhile, Staphylococcus aureus (SA) has resisted human vaccine development date. Here we show that prior SA exposure induces non-protective CD4 + T cell imprints, leading blunting protective IsdB responses. Mechanistically, these SA-experienced cells express IL-10, which further amplified by vaccination...
The imipenem and meropenem-resistant strains Citrobacter freundii HS70 Escherichia coli HS510 were isolated from patients in Shanghai, China. By isoelectric focusing, PCR amplification sequencing, these each found to produce four β-lactamases: TEM-1, KPC-3, SHV-7 CTX-M-14. A conjugation experiment plasmid restriction digestion revealed that the bla KPC-3 gene was located on same both isolates. Bidirectional primer walking sequencing showed nucleotide sequence surrounding 3.8 kb contained a...