A5031719690- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Antifungal resistance and susceptibility
University of Tübingen
2022-2025
German Center for Infection Research
2022-2024
The species- and clone-specific susceptibility of Staphylococcus cells for bacteriophages is governed by the structures glycosylation patterns wall teichoic acid (WTA) glycopolymers. glycosylation-dependent phage-WTA interactions in opportunistic pathogen epidermidis other coagulase-negative staphylococci (CoNS) have remained unknown. We report a new S. WTA glycosyltransferase TagE whose deletion confers resistance to siphoviruses such as ΦE72 but enables binding otherwise unbound...
Highlights•Receptor-binding proteins (RBPs) of 335 S aureus phages were identified•Most S. encode two distinct RBPs•15 recombinant RBPs bound to wall teichoic acid (WTA) polymers•The new PhARIS tool predicts the host binding phagesSummaryBacteriophages are crucial in bacterial communities and can be used for therapy multidrug-resistant pathogens such as Staphylococcusaureus. However, range remains difficult predict. We identified receptor-binding aureus-infecting phages, yielding 8 RBP...
Bacteria use quorum sensing (QS) to coordinate group behavior in response cell density, and some bacterial viruses (phages) also respond QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci produce AIPs which many inhibit S. aureus agr. We show that agr induction reduces expression tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification major phage receptor, wall teichoic...
Staphylococcus epidermidis is a common member of the human skin and nose microbiomes frequent cause invasive infections. Transducing phages accomplish horizontal transfer resistance virulence genes by mispackaging mobile-genetic elements, contributing to severe, therapy-refractory S. Lytic on other hand can be interesting candidates for new anti-S. phage therapies. Despite importance phages, we are only beginning unravel interactions. Recent studies shed light diversity, host range, receptor...
Staphylococcus epidermidis expresses glycerol phosphate wall teichoic acid (WTA), but some health care–associated methicillin-resistant S. (HA-MRSE) clones produce a second, ribitol (RboP) WTA, resembling that of the aggressive pathogen aureus . RboP-WTA promotes HA-MRSE persistence and virulence in bloodstream infections. We report here TarM enzyme [TarM(Se)] glycosylates with glucose, instead N -acetylglucosamine (GlcNAc) by TarM(Sa) Replacement GlcNAc glucose impairs detection human...
Abstract The species- and clone-specific susceptibility of Staphylococcus cells for bacteriophages is governed by the structures glycosylation patterns wall teichoic acid (WTA) glycopolymers. glycocodes phage-WTA interaction in opportunistic pathogen epidermidis other coagulase-negative staphylococci (CoNS) have remained unknown. We report a new S. WTA glycosyltransferase TagE whose deletion confers resistance to siphoviruses such as ΦE72 but enables binding otherwise unbound podoviruses....
Summary Wall teichoic acids (WTAs) are major surface polymers of staphylococcal pathogens and commensals, whose variable structure governs interaction with host receptors, immunoglobulins, bacteriophages. The ribitol phosphate (RboP) WTA type contributes to virulence, for instance in Staphylococcus aureus , but we lack comprehensive knowledge types cognate phages. We developed a computational pipeline identify the receptor-binding proteins (RBPs) 335 phage genomes, yielding multiple distinct...
Methicillin-resistant Staphylococcus aureus (MRSA), a major human pathogen, uses the prophage-encoded tarP gene as an important immune evasion factor. TarP glycosylates wall teichoic acid (WTA) polymers, S. surface antigens, to impair WTA immunogenicity and impede host defence. However, phages appear be restricted only few MRSA clonal lineages, including complexes (CC) 5 398, for unknown reasons. We demonstrate here that -encoding prophages can mobilized lysogenize other strains. transfer is...
Abstract Bacteria and their viruses (phages) use quorum sensing (QS) systems to coordinate group behavior. In Staphylococcus aureus , QS plays a critical role in the transition from colonization infection involves accumulation of auto-inducing peptides (AIPs). Humans animals are also colonized by non-aureus staphylococci (NAS) that produce AIPs, many which inhibit S. QS. We found induction is necessary for susceptibility lytic phage, Stab20 mixed communities with NAS producing inhibitory...