A5069120129- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Microbial infections and disease research
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bacteriophages and microbial interactions
- Ionic liquids properties and applications
- Bacterial Identification and Susceptibility Testing
- Listeria monocytogenes in Food Safety
- Spectroscopy and Chemometric Analyses
- Ruminant Nutrition and Digestive Physiology
- Protein purification and stability
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Advanced Proteomics Techniques and Applications
- Reproductive Physiology in Livestock
- interferon and immune responses
- Blood properties and coagulation
- Identification and Quantification in Food
- Essential Oils and Antimicrobial Activity
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- Bacillus and Francisella bacterial research
University of Veterinary Medicine Vienna
2015-2024
University of Veterinary Medicine
2016
TU Wien
2003-2011
University of Vienna
2008
Octapharma (Austria)
2002-2003
Staphylococcus aureus is one of the most important contagious mastitis pathogens in dairy cattle. Due to its zoonotic potential, control S. not only great economic importance industry but also a significant public health concern. The aim this study was decipher potential bovine udder associated as reservoir for contamination production and processing. From 18 farms, delivering their milk an alpine plant smeared semi-hard hard cheese. thousand hundred seventy six quarter (QM) samples all cows...
ABSTRACT Staphylococcus aureus capsular polysaccharides (CP) are important virulence factors and represent putative targets for vaccine development. Therefore, the purpose of this study was to develop a high-throughput method identify discriminate clinically S. serotypes 5, 8, NT (nontypeable). A comprehensive set clinical isolates derived from different origins control strains, representative each serotype, were used establish CP typing system based on Fourier transform infrared (FTIR)...
Salicylic acid (SAL) has recently been shown to induce biofilm formation in Staphylococcus aureus and affect the expression of virulence factors. This study was aimed investigate effect SAL on regulatory agr system its impact S. formation. The quorum-sensing system, which is a central regulator pathogenicity, plays pivotal role dispersal mature biofilms contributes creation new colonization sites. Here, we demonstrate that impairs by interfering with expression. As revealed our work,...
The objective of the present study was to examine diversity Staphylococcus aureus from mastitis milk samples cows in Rwanda.
Abstract Staphylococcus aureus causing persistent, recurrent bovine intramammary infections are still a major challenge to dairy farming. Generally, one or few clonal lineages predominant in herds, indicating animal-to-animal transfers and the existence of distinct pathotypic traits. The aim this study was determine if long term persistence spreading S. associated with specific phenotypic traits, including cellular invasion, cytotoxicity biofilm formation. Mastitis isolates were collected...
We previously reported that disruption of the yjbI gene reduced virulence Staphylococcus aureus. In this study, we found in both silkworms and mice was restored by introducing yjbH but not to genes-disrupted mutants, suggesting yjbH, downstream a two-gene operon-yjbIH, is responsible for phenomenon. further observed decrease various surface-associated proteins changes cell envelope glycostructures mutants. RNA-seq analysis revealed genes resulted differential expression broad range genes,...
Bacteria use quorum sensing (QS) to coordinate group behavior in response cell density, and some bacterial viruses (phages) also respond QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci produce AIPs which many inhibit S. aureus agr. We show that agr induction reduces expression tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification major phage receptor, wall teichoic...
Aspirin has provided clear benefits to human health. But salicylic acid (SAL) -the main aspirin biometabolite- exerts several effects on eukaryote and prokaryote cells. SAL can affect, for instance, the expression of Staphylococcus aureus virulence factors. also form complexes with iron cations it been shown that different chelating molecules diminished formation S. biofilm. The aim this study was elucidate whether content limitation caused by modify metabolism and/or metabolic regulators...
The present study was conducted from July to August 2018 on milk samples taken at dairy farms in the Northern Province and Kigali District of Rwanda order identify Staphylococcus spp. associated with bovine intramammary infection. A total 161 staphylococcal isolates originating quarter 112 crossbred cattle were included study. Antimicrobial susceptibility testing performed examined for presence various resistance genes. aureus also analyzed virulence factors, genotyped by spa typing further...
A major determinant of β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA) is the drug insensitive transpeptidase, PBP2a, encoded by mecA. Full expression phenotype requires auxiliary factors. Two such factors, factor (auxA, SAUSA300_0980) and B (auxB, SAUSA300_1003), were identified a screen against mutants with increased susceptibility to β-lactams MRSA strain, JE2. auxA auxB encode transmembrane proteins, AuxA predicted be transporter. Inactivation or enhanced...
Surface carbohydrate moieties are essential for bacterial communication, phage-bacteria and host-pathogen interaction. Most Staphylococcus aureus produce polyribitolphosphate type Wall teichoic acids (WTAs) substituted with α- and/or β-O-linked N-acetyl-glucosamine (α-/β-O-GlcNAc) residues. GlcNAc modifications have attracted particular interest, as they were shown to govern staphylococcal adhesion host cells, promote phage susceptibility conferring beta-lactam resistance an important target...
FTIR spectroscopic analysis of two IL species with similar high antimicrobial activity ([TC<sub>8</sub>MA]/[TMC<sub>16</sub>A]) revealed independently their anion different modes action against <italic>S. typhimurium</italic> through alterations in the bacterial membrane fluidity.
Pertussis is a highly contagious disease mainly caused by Bordetella pertussis. Despite the massive use of vaccines, since 1950s has become re-emergent in 2000 with shift incidence from infants to adolescents and adults. Clearly, efficacy current cellular or acellular formulated bacteria grown stirred bioreactors limited, presenting challenge for future vaccine development. For gaining insights into role B. pertussis biofilm development host colonization persistence within host, we examined...
Staphylococcus aureus is a major cause of bovine mastitis, commonly leading to long-lasting, persistent and recurrent infections. Thereby, S. constantly refines permanently adapts the udder environment. In this work, we followed within-host adaptation over course three months in naturally infected dairy cattle with chronic, subclinical mastitis. Whole genome sequence analysis revealed complete replacement initial predominant variant by another isogenic variant. We report for first time...
IL-1beta is an important proinflammatory cytokine with a major role in several inflammatory diseases. Expression of tightly regulated at the level transcription, mRNA stability, and proteolytic processing. In this study, we report that expression response to LPS also translational level. LPS-induced protein levels macrophages derived from murine bone marrow are markedly increased absence tyrosine kinase 2 (Tyk2). Increased found intra- extracellularly, irrespective efficiency We show Tyk2...
Tyrosine kinase 2 (Tyk2) is an integral part of the Janus kinase-signal transducer and activator transcription (JAK-STAT) pathway which relays intracellular signals various cytokines. Tyk2 crucially contributes to host defense mechanisms against microbial pathogens tumor surveillance but also facilitates immune pathologies. Here we investigated impact on macrophage proteome using synthetic double-stranded RNA analog polyinosinic acid-polycytidylic acid (poly(I:C)) as a mimicry viral...
Bacterial pathogens are known for their wide range of strategies to specifically adapt host environments and infection sites. An in-depth understanding these adaptation mechanisms is crucial the development effective therapeutics new prevention measures. In this study, we assessed suitability Fourier Transform Infrared (FTIR) spectroscopy monitoring metabolic adaptations bacterial pathogen Listeria monocytogenes specific genotypes exploring potential FTIR gain novel insights into...
Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia and causes significant losses in pig industry worldwide. Early host immune response crucial for further progression disease. A. either rapidly eliminated by system or switches to a long-term persistent form. To gain insight into host-pathogen interaction during early stages infection, pigs were inoculated intratracheally with serotype 2 humanely euthanized eight hours after infection. Gene expression...
Over the past decades, ionic liquids (ILs) have gained considerable attention from scientific community because of their versatile and designable properties. As a result, there are numerous IL applications, not only in organic synthesis, catalysis, or extraction but also as active pharmaceutical ingredients novel antimicrobials. While effort has been put into developing quantitative structure–activity relationship (QSAR) models for toxicity prediction, little is known about actual mode...