A5029719511- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Viral Infections and Immunology Research
- Veterinary medicine and infectious diseases
- Cell Adhesion Molecules Research
- Veterinary Oncology Research
- Xenotransplantation and immune response
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Coccidia and coccidiosis research
- Parasitic Infections and Diagnostics
- Infectious Diseases and Mycology
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Mycotoxins in Agriculture and Food
- IL-33, ST2, and ILC Pathways
University of Veterinary Medicine Vienna
2016-2025
Memorial University of Newfoundland
2011
Friedrich-Loeffler-Institut
1995-2009
University of Vienna
2005-2008
Paul Ehrlich Institut
1996-2004
Institute of Immunology
2000-2002
Universität Hamburg
2002
University Medical Center Hamburg-Eppendorf
2002
University of Tübingen
1998
Czech Academy of Sciences, Institute of Microbiology
1997
Abstract The existence of four subpopulations in resting porcine T lymphocytes is documented. In addition to the two known which are typified by a mutually exclusive expression either CD8 or CD4 differentiation antigen, − + and lymphocytes, respectively, unusual were prominent not only peripheral blood, but also lymphoid tissues: expressing neither these antigens coexpressing both antigens. While lymphoblasts have been found other species, with that phenotype without precedent among all...
The transcription factor Stat1 plays an essential role in responses to interferons (IFNs). Activation of is achieved by phosphorylation on Y701 that followed nuclear accumulation. For full transcriptional activity and biological function must also be phosphorylated S727. molecular mechanisms underlying the IFN-induced S727 are incompletely understood. Here, we show both translocation required for phosphorylation. We further mutants lacking ability stably associate with chromatin poorly...
ABSTRACT Classical swine fever (CSF) is a severe hemorrhagic disease of caused by the pestivirus CSF virus (CSFV). Amino acid exchanges or deletions introduced site-directed mutagenesis into putative active site RNase residing in glycoprotein E rns CSFV abolished enzymatic activity this protein, as demonstrated with an test suitable for detection crude cell extracts. Incorporation altered sequences infectious clone resulted recovery viable viruses upon RNA transfection, except variant...
Hog cholera, also known as classical or European swine fever, is caused by hog cholera virus, a member of the genus Pestivirus. It shown here that end stage lethal infection in natural host associated with dramatic depletion preferentially B lymphocytes circulatory system well lymphoid tissues. Already at onset disease, viral replication tissues demarcates germinal centers, and genome remains localized to site disease progresses even after morphologic disintegration follicular structure. A...
Aspirin (acetylsalicylic acid) has been used for more than 115 years in medicine. Research exists to show that aspirin antiviral effects vitro, example, by blocking influenza virus propagation via NF-κB inhibition when at high concentrations and short-term incubation steps. The aim of this study was confirm the activity against further elucidate other respiratory viruses.Tests detect were performed using plaque-reduction assays. administered virus-infected cell cultures one hour after...
Abstract Differentiation of porcine T helper cells is still poorly investigated, partly due to a lack monoclonal antibodies (mAbs) specific for molecules involved in this process. Recently, we identified mAb CD27 and showed that expressed by all naïve CD8α - but divides + into subset. In the present study, detailed phenotypical functional analyses these T-helper cell subpopulations were performed. Naïve predominantly resided various lymph nodes, whereas higher proportions found blood, spleen...
Classical swine fever virus (CSFV) -specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their activity was determined against CSFV-infected target cells obtained simian 40 (SV40) large antigen-transfected immortalized kidney a syngeneic miniature swine. Experiments separated effector cell populations revealed that the CSFV-specific mediated by...
Objectives In light of evidence suggesting that hemofiltration favorably influences septic diseases by removing sepsis mediators, the impact different modalities continuous veno-venous (CVVH) on outcome and immunologic derangements in porcine pancreatogenic was evaluated. Design Randomized, controlled intervention trial. Subjects Forty-eight minipigs either sex. Interventions Pancreatitis induced intraductal injection sodium taurocholate (4%, 1 mL/kg body weight [BW]) enterokinase (2 U/kg...
The kinetics of monocyte–macrophage differentiation was analysed using two Swine Workshop Cluster (SWC) CD molecules: SWC1 and SWC9. Myeloid cells were selected by labelling for the common myeloid antigen, SWC3. Confirmation macrophage identification used acid phosphatase phagocytosis activities. During differentiation, gradually lost. SWC9 absent on monocytes but up‐regulated early. Consequently, + − macrophages . An additional, intermediate, cell population identified as Size granularity...
Abstract The species swine provides the only example for CD2 + and − subsets of lg CD4 CD8 lymphocytes with propensity homing to lymphoid tissue (Saalmüller et al., Eur. J. Immunol. 1989. 19: 2011). That are bare marker molecules that typify T raised question whether or not this cell type is descended from lymphocyte differentiation lineage. It documented expression a phylogenetically conserved external epitope receptor γ/δ subdivides porcine into an 86D minor major subset. Expression...
The composition of the T lymphocyte population in swine is special that addition to CD4−CD8+ subpopulations, CD4−CD8− and CD4+CD8− CD4+CD8+ subpopulations are prominent peripheral circulating as well resident pools. Since same phenotypes characteristic thymic populations, it was asked whether unusual distribution may result from an emigration precursor periphery. This explanation refuted, all were found express CD1, albeit with differences antigen density, whereas extrathymic lack CD1....