A5084130326- T-cell and B-cell Immunology
- Veterinary Oncology Research
- Immune Cell Function and Interaction
- Wildlife Ecology and Conservation
- Infectious Diseases and Mycology
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Genetic diversity and population structure
- Immunotherapy and Immune Responses
- Microbial infections and disease research
- Xenotransplantation and immune response
- Animal Virus Infections Studies
- Ecology and biodiversity studies
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Animal Ecology and Behavior Studies
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Identification and Quantification in Food
- Genetic and phenotypic traits in livestock
- Veterinary medicine and infectious diseases
- Influenza Virus Research Studies
- T-cell and Retrovirus Studies
University of Veterinary Medicine Vienna
2015-2024
Institute of Immunology
2009-2018
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2008
Universidad de Oviedo
2008
Office National de la Chasse et de la Faune Sauvage
2008
Czech Academy of Sciences, Institute of Vertebrate Biology
2008
Medical University of Vienna
2004-2006
University of Vienna
2000-2003
This report summarizes the new swine leukocyte antigen (SLA) allele sequences and haplotypes designated by SLA Nomenclature Committee of International Society for Animal Genetics. There have been 74 alleles, comprising 18 SLA-1 11 SLA-2 six SLA-3 two SLA-6 one SLA-DRA allele, 20 SLA-DRB1 three SLA-DQA alleles 13 SLA-DQB1 alleles. Twelve class I four II also designated. is first official update since 2005 reports on nomenclature factors systems. recent updates to Immunopolymorphism...
Pigs are natural hosts for influenza A viruses and play a critical role in epidemiology. However, little is known about their influenza-evoked T-cell response. We performed thorough analysis of both the local systemic response virus-infected pigs, addressing kinetics phenotype as well multifunctionality (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], interleukin-2 [IL-2]) cross-reactivity. total 31 pigs were intratracheally infected with an H1N2 swine virus (FLUAVsw)...
Unlike mice and humans, porcine γδ T cells represent a prominent subset of in blood secondary lymphatic organs. GATA-3, T-bet Eomesodermin (Eomes) are transcription factors with crucial functions T-cell development functional differentiation, but their expression has not been investigated so far. We analyzed the these thymocytes, mature from blood, spleen, lymph nodes lung tissue as well vitro stimulated on protein level by flow cytometry. GATA-3 was present more than 80% all γδ-thymocytes....
Abstract One of the prerequisites for successful organ xenotransplantation is a reasonable size match between porcine and recipient's to be replaced. Therefore, selection suitable genetic background source pigs important. In this study, we investigated body growth, cardiac function, diversity colony Auckland Island established at Center Innovative Medical Models (CiMM), LMU Munich. Male female pig kidney cells (selected free endogenous retrovirus C) were imported from New Zealand, founder...
Immune rejection remains the major obstacle to long-term survival of allogeneic lung transplants. The expression histocompatibility complex molecules and minor antigens triggers immune responses that can lead allograft rejection. Transplant outcomes therefore depend on immunosuppression, which is associated with severe side effects. To address this problem, we investigated effect genetically engineered transplants permanently down-regulated swine leukocyte antigen (SLA) prevent in a porcine...
Abstract The classification of the Pteriomorphia, a major division Bivalvia with Ordovician origins, is controversial both among palaeontologists and neontologists. To elucidate phylogenetic relationships new near-complete 18S rDNA sequences 26 three Protobranchia, Heterodonta, one Anomalodesmata Scaphopoda were obtained, aligned 71 other published molluscan sequences, analysed parsimony, maximum likelihood spectral analysis. Although appear diphyletic due to heterogeneity substitution rates...
The chamois, distributed over most of the medium to high altitude mountain ranges southern Eurasia, provides an excellent model for exploring effects historical and evolutionary events on diversification. Populations have been grouped into two species, Rupicapra pyrenaica from southwestern Europe R. rupicapra eastern Europe. However, a previous study cytochrome b revealed that proposed species were non-monophyletic. reconstruction phylogenetic relationships between animal often depends...
Although swine are natural hosts for influenza A viruses, the porcine T-cell response to virus (FLUAVsw) infection has been poorly characterized so far. We have studied Ki-67 expression and FLUAVsw-specific production of IFN-γ, TNF-α IL-2 in CD4+ CD8β+ T cells isolated from piglets that had intratracheally infected with a H1N2 FLUAVsw isolate. IFN-γ+TNF-α+IL-2+ multifunctional were present blood all animals at one or two weeks after primary their frequency increased four out six homologous...
The CD3-NKp46+ phenotype is frequently used for the identification of NK cells in various mammalian species. Recently, NKp46 expression was analyzed more detail swine. It could be shown that besides lymphocytes, a small but distinct population CD3+NKp46+ exists. In this study we report low frequencies lymphocytes blood, lymph nodes and spleen increased non-lymphatic organs like liver lung. Phenotypic analyses showed majority co-expressed CD8αβ heterodimer, while minor subset expressed TCR-γδ...
Abstract Differentiation between resident mature lymphocyte populations and small cell lymphoma cannot be made by cytological review alone remains challenging in histopathological review. These cases warrant application of complementary tools like PCR ‐based immunoglobulin ( IG ) T‐cell receptor TCR clonality testing for confirmation. In this prospective study, diagnostic sensitivity specificity different primer sets routine diagnosis feline gamma TCRG complete heavy chain IGH gene...
The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate very high degree of homozygosity across genome, including major histocompatibility complex (MHC) region, but confirmation at specific MHC loci was lacking. Using both direct sequencing and PCR‐based sequence‐specific typing, we confirm that pigs are essentially homozygous their formalise haplotype as Hp‐55.6. This enhances utility useful biomedical model for...
A substantial fraction of vertebrate and invertebrate genomes is composed mobile elements their derivatives. One the most intensively studied transposon families, P Drosophila, was thought to exist exclusively in dipteran insects. Based on data provided by human genome project, 2001 our group has identified a element–homologous sequence genome. This gene, named Phsa, 19,533 nucleotides long, comprises six exons five introns, encodes protein still unknown function with length 903 amino acid...
Objectives The purpose of this study was to specify lymphoma subtypes according the World Health Organization (WHO) classification in a group cats and investigate their potential prognostic value. Methods Records from University Veterinary Medicine Vienna suffering were reviewed retrospective study. To diagnose various specified WHO classification, histopathological immunohistochemical examinations, as well clonality assays some cases, performed. Results Of 30 included classified guidelines,...
The porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of one most economically important diseases affecting swine worldwide. Efforts to develop a next-generation vaccine have largely focused on envelope glycoproteins target virus-neutralizing antibody responses. However, these approaches failed demonstrate necessary efficacy progress toward market. T cells are crucial control many viruses through cytolysis cytokine secretion. Since PRRSV infection not dependent...
The chamois provides an excellent model for exploring the effect of historical and evolutionary events on diversification. We investigate cytochrome b (cytb) sequences in 10 recognized subspecies Rupicapra classified within 2 species: pyrenaica, with parva, ornata, rupicapra, cartusiana, tatrica, carpatica, balcanica, asiatica, caucasica. A fragment 349 bp cytb was sequenced 189 individuals. identified 3 lineages: Clade West Iberia Western Alps; Central Apennines Massif Chartreuse; East...
Abstract The protozoan parasite Histomonas meleagridis is the causative agent of re-emerging disease histomonosis chickens and turkeys. Due to parasite’s extracellular occurrence, a type-2 differentiation H. -specific T cells has been hypothesized. In contrast, recent study suggested that IFN-γ mRNA + are involved in protection against histomonosis. However, phenotype cytokine production profile still awaits elucidation. this work, clonal cultures virulent monoxenic strain were used for...