A5083684557- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Microbial infections and disease research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Xenotransplantation and immune response
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Toxoplasma gondii Research Studies
- Mycobacterium research and diagnosis
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Immune responses and vaccinations
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Parasitic Infections and Diagnostics
- Genetic Mapping and Diversity in Plants and Animals
- Parasitic Diseases Research and Treatment
- Viral Infections and Immunology Research
Agricultural Research Service
2016-2025
Beltsville Agricultural Research Center
2016-2025
United States Department of Agriculture
2011-2024
Barco (Belgium)
2023
Iowa State University
2019
University of Alberta
2019
United States Department of State
2011-2014
Brazilian Agricultural Research Corporation
2013
South Dakota State University
2013
VIB-VUB Center for Structural Biology
2012
Abstract Partially inbred miniature swine have been developed in this laboratory as a large animal model for immunologic studies. For many of these studies, source quantities well standardized antibodies to lymphocyte cell surface markers would be extremely helpful. Screening panel murine anti-human monoclonal (MAb) anti-pig cross-reactions was nonproductive. We therefore prepared MAb from mice immunized with pig thymocytes. Twenty-six stable were produced that reacted...
Abstract Two murine monoclonal antibodies reactive with porcine nylon wool-nonadherent T cells were examined by flow microfluorometry analysis for cellular distribution, and functional their effect on MLR CTL assays. The two determinants recognized, called PT4 PT8, shown to be expressed almost mutually exclusive cell populations, only 6 15% of expressing both antigens. Among the that did not express either or 5% surface Ig+. In assays, proliferated PHA, hen egg lysozyme, alloantigens blocked...
To define the infectiousness of VEG strain Toxoplasma gondii, 42 pigs were fed doses estimated at 10, 1, or < 1 mouse infective oocysts. They killed 38-99 days after inoculation and 50 g tissues from their tongue, heart, brain individually homogenized in acidic pepsin solution bioassayed mice. Pools brain, skeletal muscle (total 500 g) cats. gondii was isolated by bioassays mice cats 13 14 10 oocysts, oocyst, 4 "less than" indicating high infectivity T. to pigs. All infected developed...
Porcine reproductive and respiratory syndrome (PRRS) causes decreased performance in breeding animals increased problems growing animals, which result significant economic losses the swine industry. Vaccination has generally not been effective prevention of PRRS, partially because rapid mutation rate evolution virus. The objective current study was to discover genetic basis host resistance or susceptibility PRRS virus through a genome-wide association using data from Host Genetics Consortium...
Evidence is reported to support the following conclusions: (i) The presence of a specific hepatic receptor capable recognizing and binding galactose-terminated glycoproteins responsible for maintaining near zero levels circulating asialoglycoproteins in mammalian serum. (ii) absence this galactose-specific protein avian, presumably reptilian, liver correlated with high serum these species. (iii) evolutionary significance observations reinforced by recognition an avian terminal,...
ABSTRACT Human infectious diseases have been studied in pigs because the two species common microbial, parasitic, and zoonotic organisms, but there has no systematic evaluation of cytokine gene expression response to agents porcine species. In this study, were inoculated with clinically economically important parasites, Toxoplasma gondii Ascaris suum , 11 different tissues for 20 swine Th1/Th2-related cytokines, receptors, markers immune activation evaluated by real-time PCR. A generalized...
ABSTRACT A highly sensitive and specific method has been developed to reproducibly detect quantitate Toxoplasma gondii burden in animal tissue samples using T. ITS1-derived primers a fluorogenic probe via real-time PCR. Assay specificity was confirmed against panel of DNA from other common protozoa as well host tissue. This Toxo TaqMan assay able little 0.1 pg genomic DNA, which is equivalent 1 bradyzoite, dynamic range detection 100 ng fg DNA. Tissues experimentally infected mice pigs...
ABSTRACT The recent emergence of a unique group North American type 1 porcine reproductive and respiratory syndrome virus (PRRSV) in the United States presents new disease control problems for swine industry that has already been impacted seriously by 2 PRRSV. In this study, full-length cDNA infectious clone was generated from low-virulence PRRSV isolate, SD01-08. vitro studies demonstrated cloned maintained growth properties similar to those parental virus. Virological, pathological,...
Offspring of heterozygous parents derived from three herds miniature swine, each which is homozygous at the major histocompatibility complex (MHC), were screened for recombination within MHC. The swine typed serologically weaning and later by mixed lymphocyte reaction (MLR). Two intra-MHC recombinants discovered, both involved exchange D region specificities without apparent dissociation ABC specificities, confirming localization SLA-D outside SLA-ABC regions. first recombinant was offspring...
REVIEW article Front. Genet., 14 December 2012Sec. Livestock Genomics https://doi.org/10.3389/fgene.2012.00260
The highly polymorphic swine leucocyte antigen (SLA) genes are among the most important determinants of immune responses to disease and vaccines. Accurate effective SLA genotyping methods required understand how gene polymorphisms affect immunity, especially in outbred pigs with diverse genetic backgrounds. In this study, we present a simple rapid molecular-based typing system for characterizing class II alleles DRB1, DQB1 DQA loci. This utilizes set 47 sequence-specific PCR primers...
This report summarizes the new swine leukocyte antigen (SLA) allele sequences and haplotypes designated by SLA Nomenclature Committee of International Society for Animal Genetics. There have been 74 alleles, comprising 18 SLA-1 11 SLA-2 six SLA-3 two SLA-6 one SLA-DRA allele, 20 SLA-DRB1 three SLA-DQA alleles 13 SLA-DQB1 alleles. Twelve class I four II also designated. is first official update since 2005 reports on nomenclature factors systems. recent updates to Immunopolymorphism...
Abstract Background Host genetics has been shown to play a role in porcine reproductive and respiratory syndrome (PRRS), which is the most economically important disease swine industry. A region on Sus scrofa chromosome (SSC) 4 previously reported have strong association with serum viremia weight gain pigs experimentally infected PRRS virus (PRRSV). The objective here was identify haplotypes associated favorable phenotype, investigate additional genomic regions host response PRRSV, determine...
Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type single cell levels. First, eight types isolated from mononuclear (PBMCs) by sorting, representing Myeloid, NK specific populations T B-cells. Transcriptomes for each population generated RNA-seq with 10,974 expressed genes detected. Pairwise comparisons between revealed expression, while...