A5011158089- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Renal Transplantation Outcomes and Treatments
- Mycobacterium research and diagnosis
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Tissue Engineering and Regenerative Medicine
- Cardiac Structural Anomalies and Repair
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Advanced Biosensing Techniques and Applications
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- SARS-CoV-2 and COVID-19 Research
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Multiple Myeloma Research and Treatments
- Animal Genetics and Reproduction
- Blood donation and transfusion practices
- Mechanical Circulatory Support Devices
- Genetic Mapping and Diversity in Plants and Animals
Gift of Hope Organ and Tissue Donor Network
2019-2025
Michigan State University
2022-2025
Gift of Life Michigan
2012-2022
Michigan United
2018
Cedars-Sinai Medical Center
2013
Capricor Therapeutics (United States)
2013
University of Michigan–Ann Arbor
2008-2010
Chinese University of Hong Kong
2004-2010
Prince of Wales Hospital
2010
Baylor University
2009-2010
The IPD-MHC Database project (http://www.ebi.ac.uk/ipd/mhc/) collects and expertly curates sequences of the major histocompatibility complex from non-human species provides infrastructure tools to enable accurate analysis. Since first release database in 2003, has grown currently hosts a number specific sections, with more than 7000 alleles 70 species, including primates, canines, felines, equids, ovids, suids, bovins, salmonids murids. These are curated made publicly available through an...
African swine fever (ASF) is a lethal haemorrhagic disease of domestic pigs for which there no vaccine. Strains the virus with reduced virulence can provide protection against related virulent strains ASFV, but not 100% and are concerns about safety profile such viruses. However, they useful tool understanding immune response to ASFV previous studies using low isolate OUR T88/3 have shown that CD8+ cells crucial protection. In order develop vaccine stimulates an effective anti-ASFV T-cell we...
Magnetic resonance imaging (MRI) in the CArdiosphere-Derived aUtologous stem CElls to reverse ventricUlar dySfunction (CADUCEUS) trial revealed that cardiosphere-derived cells (CDCs) decrease scar size and increase viable myocardium after myocardial infarction (MI), but MRI has not been validated as an index of regeneration cell therapy. We tested validity contrast-enhanced quantifying scarred therapy a porcine model convalescent MI.
Classical approaches to African swine fever virus (ASFV) vaccine development have not been successful; inactivated does provide protection and use of live attenuated viruses generated by passage in tissue culture had a poor safety profile. Current (ASF) research focuses on the modified targeted gene deletion or subunit vaccines. The latter approach would be differentiation vaccinated from infected animals (DIVA)-compliant, but information which viral proteins include is lacking. Our previous...
Decisions to transplant organs from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test-positive (NAT+) donors must balance risk of donor-derived transmission events (DDTE) with the scarcity available organs.Organ Procurement and Transplantation Network (OPTN) data were used compare organ utilization recipient outcomes between SARS-CoV-2 NAT+ NAT- donors. was defined by either a positive upper or lower tract (LRT) sample within 21 days procurement. Potential DDTE...
The highly polymorphic swine leucocyte antigen (SLA) genes are among the most important determinants of immune responses to disease and vaccines. Accurate effective SLA genotyping methods required understand how gene polymorphisms affect immunity, especially in outbred pigs with diverse genetic backgrounds. In this study, we present a simple rapid molecular-based typing system for characterizing class II alleles DRB1, DQB1 DQA loci. This utilizes set 47 sequence-specific PCR primers...
This report summarizes the new swine leukocyte antigen (SLA) allele sequences and haplotypes designated by SLA Nomenclature Committee of International Society for Animal Genetics. There have been 74 alleles, comprising 18 SLA-1 11 SLA-2 six SLA-3 two SLA-6 one SLA-DRA allele, 20 SLA-DRB1 three SLA-DQA alleles 13 SLA-DQB1 alleles. Twelve class I four II also designated. is first official update since 2005 reports on nomenclature factors systems. recent updates to Immunopolymorphism...
Transplantation of gene-corrected autologous hepatocytes can cure metabolic disease in a preclinical pig model hereditary tyrosinemia type 1.
The highly polymorphic swine leucocyte antigen (SLA) genes are one of the most important determinants in immune responses to infectious diseases, vaccines, and transplantation success. Study SLA influence requires accurate effective typing methods. We developed a simple rapid method type alleles at three classical class I loci (SLA-1, SLA-3 SLA-2) using PCR-sequence-specific primer (PCR-SSP) strategy. This system relies on 47 discriminatory PCR pairs designed amplify by groups that have...
Syngeneic iPSC–derived neurons survive and mature without immunosuppression after grafting into the spinal cord of adult pigs.
Abstract Background Characterization of transplant transmitted infections associated with drowned organ donors and the recipients is not well delineated, posing challenges for optimal management prophylaxis strategies. Broader sharing may pose additional considerations due to pathogen variation. Methods We reviewed 22 potential donor disease transmission events (PDDTEs) from reported Organ Procurement Transplantation Network (OPTN) ad hoc Disease Transmission Advisory Committee (DTAC)...
The IPD-MHC Database is the official repository for non-human MHC sequences, overseen and supported by Comparative Nomenclature Committee, providing access to curated data associated analysis tools. To address increasing amount complexity of being submitted, an entirely upgraded version was recently released maintain as central platform comparison data. As a consequence, new level nomenclature standardisation required between different species enable submission allow unambiguous inter-...
Partial heart transplantation is a new approach to deliver growing valve implants. transplants differ from because only the part of containing necessary transplanted. This allows partial grow, similar valves in transplants. However, transplant biology remains unexplored. critical barrier progress field. Without knowledge about specific transplantation, children with are empirically treated like known grow. In order field, an animal model for necessary. Here, we contribute our surgical...
Porcine reproductive and respiratory syndrome virus (PRRSV) infection is difficult to control because the undergoes antigenic variation during also modulates protective host immune response. Although current vaccines do not provide full protection, they have provided insight into mechanisms of protection. Live PRRSV induce partial protection before appearance neutralizing antibody, suggesting cell-mediated immunity or other may be involved. Herein, we demonstrate recovery from associated...
Infusion of allogeneic cardiosphere-derived cells (allo-CDCs) postreperfusion elicits cardioprotective cellular postconditioning in pigs with acute myocardial infarction. However, the long-term effects allo-CDCs have not been assessed. We performed a placebo-controlled pivotal study for evaluation, as well shorter-term mechanistic studies.Minipigs underwent 1.5-hour mid-left anterior descending balloon occlusion followed by reperfusion and were randomized to receive intracoronary or vehicle...
The Babraham pig is a highly inbred breed first developed in the United Kingdom approximately 50 years ago. Previous reports indicate very high degree of homozygosity across genome, including major histocompatibility complex (MHC) region, but confirmation at specific MHC loci was lacking. Using both direct sequencing and PCR‐based sequence‐specific typing, we confirm that pigs are essentially homozygous their formalise haplotype as Hp‐55.6. This enhances utility useful biomedical model for...
Pigs with severe combined immunodeficiency (SCID) are an emerging biomedical animal model. Swine anatomically and physiologically more similar to humans than mice, making them invaluable tool for preclinical regenerative medicine cancer research. One essential step in further developing this model is the immunological humanization of SCID pigs. In work we have generated T
A total of 53 alleles at five highly polymorphic swine leucocyte antigen (SLA) loci (SLA-1, SLA-3, SLA-2, SLA-DRB1, and SLA-DQB1) were identified in eight commercially available porcine cell lines (ESK-4, LLC-PK1, MPK, PK13, PK15, PT-K75, SK-RST, ST). This information is essential for the use these to understand role SLA genes proteins models transplantation, xenotransplantation, immune responses infectious diseases vaccines. The ready availability also makes them a good source reference DNA...