A5086250562- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- Vector-borne infectious diseases
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Respiratory viral infections research
- Viral Infectious Diseases and Gene Expression in Insects
- Parasitic Diseases Research and Treatment
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Insect and Pesticide Research
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Parasites and Host Interactions
- Microbial infections and disease research
- Poxvirus research and outbreaks
The Pirbright Institute
2016-2024
University of Surrey
2015-2022
Animal and Plant Health Agency
2012-2021
International Livestock Research Institute
2006-2016
Guildford Hospital
2016
University of Córdoba
2013
University of Birmingham
2012
Veterinary Medicines Directorate
2008-2011
Liverpool School of Tropical Medicine
1999-2006
University of Liverpool
2000-2006
Abstract There is need for effective and affordable vaccines against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a protein nanoparticle vaccine SARS-CoV-2. The based on display of coronavirus spike glycoprotein receptor-binding domain (RBD) synthetic virus-like particle (VLP) platform, SpyCatcher003-mi3, using SpyTag/SpyCatcher technology. Low doses RBD-SpyVLP in prime-boost regimen induce strong neutralising antibody response mice pigs that superior convalescent...
Abstract Clinical development of the COVID-19 vaccine candidate ChAdOx1 nCoV-19, a replication-deficient simian adenoviral vector expressing full-length SARS-CoV-2 spike (S) protein was initiated in April 2020 following non-human primate studies using single immunisation. Here, we compared immunogenicity one or two doses nCoV-19 both mice and pigs. Whilst dose induced antigen-specific antibody T cells responses, booster immunisation enhanced particularly pigs, with significant increase...
We investigate electropolymerized molecularly imprinted polymers (E-MIPs) for the selective recognition of SARS-CoV-2 whole virus. E-MIPs with pseudoparticles (pps) were electrochemically deposited onto screen printed electrodes by reductive electropolymerization, using water-soluble N-hydroxmethylacrylamide (NHMA) as functional monomer and crosslinked N,N'-methylenebisacrylamide (MBAm). showed selectivity template pps, an imprinting factor 3:1, specificity (significance = 0.06) when...
East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva , is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation unique cancer-like condition that reversible with removal. Schizont-infected cell-directed CD8 + cytotoxic T lymphocytes (CTL) constitute dominant protective bovine immune response after single exposure to infection. However, schizont antigens targeted T. -specific CTL are undefined....
Pre-emptive culling is becoming increasingly questioned as a means of controlling animal diseases, including classical swine fever (CSF). This has prompted discussions on the use emergency vaccination to control future CSF outbreaks in domestic pigs. Despite long history safe endemic areas, there paucity data aspects important strategies, such how rapidly CSFV vaccines would protect against transmission, and if this protection equivalent for all viral genotypes, highly divergent genotype 3...
African swine fever (ASF) is a devastating disease for which there no vaccine available. The ASF virus (ASFV) primarily infects cells of the myeloid lineage and this tropism thought to be crucial pathogenesis. A detailed in vitro characterization interactions virulent Sardinian isolate (22653/14) tissue culture adapted avirulent strain (BA71V) ASFV with porcine monocytes, un-activated (moMΦ), classically (moM1) alternatively (moM2) activated monocyte-derived macrophages was conducted an...
Background Theileria parva causes an acute fatal disease in cattle, but infections are asymptomatic the African buffalo (Syncerus caffer). Cattle can be immunized against parasite by infection and treatment, immunity is partially strain specific. Available data indicate that CD8+ T lymphocyte responses mediate protection and, recently, several antigens recognised cells have been identified. This study set out to determine nature extent of polymorphism two these antigens, Tp1 Tp2, which...
Porcine reproductive and respiratory syndrome (PRRS) continues to be the most economically important disease of swine worldwide. The appearance highly pathogenic PRRS virus (PRRSV) strains in Europe Asia has raised concerns about this initiated increased efforts understand pathogenesis. In study, we have compared pathology distribution tissues pigs experimentally inoculated with three different genotype 1 PRRSV isolates. Sixty 5-week-old were intranasally a) Lelystad (LV), b) a field strain...
Monocyte-derived macrophages (MoMØ) and monocyte-derived dendritic cells (MoDC) are two model systems well established in human rodent that can be used to study the interaction of pathogens with host cells. Porcine reproductive respiratory syndrome virus (PRRSV) is known infect myeloid cells, such as (MØ) (DC). Therefore, this aimed establish for differentiation characterization MoMØ MoDC subsequent infection PRRSV-1. M-CSF differentiated were stimulated activators classical (M1) or...
Despite the widespread use of live attenuated PPRV vaccines, this is first systematic analysis immune response elicited in small ruminants. These data will help establishment immunological determinants protection, an important step development new especially DIVA vaccines using alternative vaccination vectors. This study also controlled test ability two major used against virulent strains from all genetic lineages virus, showing conclusively complete cross-protective these vaccines.
African swine fever (ASF) is a devastating disease for which there no vaccine. The ASF virus (ASFV) can infect dendritic cell (DC), but despite the critical role these cells play in induction of adaptive immunity, few studies investigated their response to ASFV infection. We characterized vitro interactions porcine monocyte-derived DCs (moDC) with virulent (22653/14), low (NH/P68) and an avirulent (BA71V) strain. At high multiplicity infection (MOI = 1), all three strains infected immature...
African swine fever (ASF) poses a severe threat to the global pig industry for which currently there is no available vaccine. The aetiological ASF virus (ASFV) has predilection cells of myeloid lineage, however little known about its interaction with polarised macrophages. This study focused on in vitro interactions porcine monocyte-derived un-activated (moMΦ), classically (moM1), alternatively (moM2), and IFN-a-activated macrophages two genotype I ASFV strains: virulent 22653/14 attenuated...
The pig is an important agricultural species and powerful biomedical model. We have established the pig, a large natural host animal for influenza with many physiological similarities to humans, as robust model testing therapeutic potential of monoclonal antibodies. Antibodies provide protection through neutralization recruitment innate effector functions Fc domain. However very little known about Fc-mediated porcine IgG subclasses. generated 8 subclasses two anti hemagglutinin characterized...
Cattle possess three IgG subclasses. However, the key immune functions, including complement and NK cell activation, enhancement of phagocytosis, are not fully described for bovine IgG1, 2 3. We produced chimeric monoclonal antibodies (mAbs) consisting a defined variable region linked to constant regions 3, expressed His-tagged soluble recombinant Fc gamma receptors (FcγRs) IA (CD64), IIA (CD32A), III (CD16) Fcγ2R. Functional assays using bovinized mAbs were developed. IgG1 IgG3, but IgG2,...
ABSTRACT Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted cells from T. -immune cattle of different major histocompatibility complex (MHC) genotypes have identified, raising prospect developing a subunit vaccine. To facilitate further dissection specificity protective T-cell responses and assist in assessment vaccination, we set out identify epitopes recognized these their MHC restriction...
Abstract Although immunodominance of CD8 + T‐cell responses is a well‐recognised feature viral infections, its role in to more antigenically complex pathogens less clear. In previous studies we have observed that Theileria parva exhibit different patterns parasite strain specificity cattle MHC genotypes. the current study, demonstrated animals homozygous for A10 and A18 haplotypes detectable only one 5 T. antigens. Over 60% responding T cells from recognised defined epitopes Tp1 Tp2...
ABSTRACT Vaccination with live attenuated classical swine fever virus (CSFV) induces solid protection after only 5 days, which has been associated virus-specific T cell gamma interferon (IFN-γ) responses. In this study, we employed flow cytometry to characterize responses following vaccination and subsequent challenge infections virulent CSFV. The CD3 + CD4 − CD8 hi population was the first major source of CSFV-specific IFN-γ. A proportion these cells showed evidence for cytotoxicity, as...
ABSTRACT There is dire need for an effective and affordable vaccine against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a modular virus-like particle candidate displaying spike glycoprotein receptor-binding domain (RBD) using SpyTag/SpyCatcher technology (RBD-SpyVLP). Low doses of RBD-SpyVLP in prime-boost regimen induced strong neutralising antibody response mice pigs that was superior convalescent human sera. We evaluated quality ACE2 blocking neutralisation cell...
Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs humans. A high fatality rate broad host tropism makes NiV a serious public animal health concern. There therefore urgent need for vaccines to protect animals In this study we investigated the immunogenicity bovine herpesvirus (BoHV-4) vectors expressing either attachment (G) or fusion (F) glycoproteins, BoHV-4-A-CMV-NiV-GΔTK BoHV-4-A-CMV-NiV-FΔTK, respectively pigs. The were...