A5028515943- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Supramolecular Self-Assembly in Materials
- Silk-based biomaterials and applications
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Dental materials and restorations
- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- biodegradable polymer synthesis and properties
- Nanoparticle-Based Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Polydiacetylene-based materials and applications
- Additive Manufacturing and 3D Printing Technologies
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Advanced Cellulose Research Studies
- Spine and Intervertebral Disc Pathology
- Lipid Membrane Structure and Behavior
- Facial Trauma and Fracture Management
- Osteoarthritis Treatment and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Model-Driven Software Engineering Techniques
University of Manchester
2016-2025
Henry Royce Institute
2020-2025
Animal and Plant Health Agency
2011-2024
Veterinary Medicines Directorate
2005-2007
University Ucinf
2006
Imperial College London
2002-2004
University of Nottingham
2001-2004
Chelsea and Westminster Hospital
2003
Nottinghamshire Healthcare NHS Foundation Trust
2002
Queen's Medical Centre
2001-2002
A number of short peptide amphiphiles consisting dipeptides linked to fluorenylmethoxycarbonyl spontaneously form fibrous hydrogels under physiological conditions (see figure). The structural and physical properties these gels are dictated by the amino acid sequence building blocks, support three-dimensional cell culture chondrocytes.
We highlight recent developments in hydrogel materials with biological responsiveness built in. These ‘smart’ biomaterials change properties response to selective recognition events. When exposed a target (nutrient, growth factor, receptor, antibody, enzyme, or whole cell), molecular events trigger changes interactions that translate into macroscopic responses, such as swelling/collapse solution-to-gel transitions. The transitions may be used directly optical readouts for biosensing, linked...
Abstract Collagen has been investigated as a potential natural biomaterial, because of its occurrence in the extracellular matrix. requires crosslinking this context, by reagents that are often cytotoxic. Glutaraldehyde is one such agent potentially The aim study was to determine cause poor cell attachment and growth on collagen/poly(vinyl alcohol) bioartificial composite films, when crosslinked with glutaraldehyde. Dehydrothermal used comparison. Human osteoblasts were observed undergo...
Abstract Highly porous poly( DL ‐lactic acid) (PDLLA) foams and Bioglass®‐filled PDLLA composite were characterized evaluated in vitro as bone tissue engineering scaffolds. The hypothesis was that the combination of with Bioglass® a structure would result bioresorbable bioactive composite, capable supporting osteoblast adhesion, spreading viability. Composite unfilled incubated simulated body fluid (SBF) at 37°C to study degradation polymer detect hydroxyapatite (HA) formation, which is...
Abstract Human primary osteoblast responses to smooth and roughened bioactive glass of 45S5 (Bioglass™) composition (46.1% SiO 2 , 26.9% CaO, 2.6% P O 5 24.4% Na O) were analysed in vitro . The rough surfaces had R a values peak valley distances 0.04, 4.397, 2.027, 21.328 μm, respectively. Cell attachment morphology was observed using phalloidin staining the actin cytoskeleton revealed significant differences between surfaces. Cells that spiky appearance on compared surface formed an...
Radially oriented submonolayer surfaces of 10-15 nm diameter cellulose nanowhiskers (CNWs) were prepared by spin-coating. The response myoblasts (muscle cells) to the was assessed using atomic force microscopy (AFM), immunocytochemistry, and image analysis. Despite small size CNWs, along CNW surfaces. Upon differentiation, produced striking radial patterns myotubes, following pattern CNWs. This facile method nanopatterning may be applied where directed growth tissue is required shows for...
Cell-based therapies for regeneration of intervertebral discs are regarded to hold promise degenerative disc disease treatment, a condition that is strongly linked lower back pain. A de novo self-assembling peptide hydrogel (SAPH), chosen its biocompatibility, tailorable properties and nanofibrous architecture, was investigated as cell carrier scaffold nucleus pulposus (NP) tissue engineering. Oscillatory rheology determined the system would likely be deliverable via minimally invasive...
Nano-sized hydroxyapatite (HA) powders were produced by a hydrothermal method and precipitation method. Spark plasma sintering (SPS) was used to fabricate nanostructured HA (NHA) using nano-sized as precursor. Conventional employed produce microstructured (MHA). Characteristics of bulk ceramics after investigated XRD, FTIR, SEM, TEM, particle size distribution, AFM. Dense compacts consisting equiaxed grains with an average grain approximately 100 nm obtained SPS. Human osteoblasts cultured...
A thermoreversible fibrillar hydrogel has been formed from an aqueous lysozyme solution in the presence of dithiothreitol (DTT). Its physical properties and potential as a tissue engineering scaffold have explored. Hydrogels were prepared by dissolving 3 mM protein 20 DTT/water mixture, heating to 85 °C cooling at room temperature. No gel was observed for equivalent sample without DTT. The elastic nature confirmed rheology, storage modulus our found be same order magnitude other cross-linked...
The design of self-assembled peptide-based structures for three-dimensional cell culture and tissue repair has been a key objective in biomaterials science decades. In search the simplest possible peptide system that can self-assemble, we discovered combinations di-peptides are modified with aromatic stacking ligands could form nanometre-sized fibres when exposed to physiological conditions. For example, demonstrated number Fmoc (fluoren-9-ylmethyloxycarbonyl) di- tri-peptides highly ordered...
An attractive strategy for the regeneration of tissues has been use extracellular matrix analogous biomaterials. Peptide-based fibrillar hydrogels have shown to mimic structure offering cells a niche undertake their physiological functions. In this study, capability an ionic-complementary peptide FEFEFKFK (F, E, and K are phenylalanine, glutamic acid, lysine, respectively) hydrogel host human mesenchymal stem in three dimensions induce osteogenic differentiation is demonstrated. Assays...