A5042931696- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Cervical Cancer and HPV Research
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- T-cell and Retrovirus Studies
- Infectious Diseases and Mycology
- Toxoplasma gondii Research Studies
- Virus-based gene therapy research
- Milk Quality and Mastitis in Dairy Cows
- Bacteriophages and microbial interactions
- Rabies epidemiology and control
- Poxvirus research and outbreaks
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Cancer-related Molecular Pathways
- Blood groups and transfusion
- Molecular Biology Techniques and Applications
- Mycobacterium research and diagnosis
- Myxozoan Parasites in Aquatic Species
University of Sassari
2015-2024
Sheep retroviruses can be used to map the selective preferences of early farmers and trace livestock movements across Europe.
African swine fever, a devastating disease for domestic pigs and wild boar, is currently spreading in Europe, Russia, China, becoming global threat with huge economic ecological consequences. One interesting aspect of ASFV biology the molecular mechanism leading to high virulence some strains compared more attenuated strains, which produce subclinical infections. In this work, we show that presently circulating virulent Armenia/07 virus blocks synthesis IFN-β, key mediator between innate...
The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, was investigated in Sardinia using molecular approach. Phylogenetic analysis revealed that Sardinian strains are genetically distinct from the two lineages previously described Europe and closely related to isolated different areas United States.
Few data are available on the prevalence and molecular typing of species belonging to genus Anaplasma in Mediterranean ruminants. In this study, PCR analysis sequencing both 16S rRNA groEL genes were combined investigate presence, prevalence, traits spp. ruminants sampled Island Sardinia, chosen as a subtropical representative area. The results demonstrate high ruminants, with animals infected by at least four six (Anaplasma marginale, A. bovis, ovis, phagocytophilum). Moreover, host number...
African swine fever (ASF) is a devastating disease for which there no vaccine available. The ASF virus (ASFV) primarily infects cells of the myeloid lineage and this tropism thought to be crucial pathogenesis. A detailed in vitro characterization interactions virulent Sardinian isolate (22653/14) tissue culture adapted avirulent strain (BA71V) ASFV with porcine monocytes, un-activated (moMΦ), classically (moM1) alternatively (moM2) activated monocyte-derived macrophages was conducted an...
African swine fever (ASF) is a notifiable infectious disease, caused by the ASF virus (ASFV), which DNA belonging to family Asfarviridae, genus Asfivirus. This disease has gained importance in last decade after its spread several countries Eastern and Central Europe, more recently, China. Despite efforts made eradicate it, still present on Mediterranean island of Sardinia (Italy) been since 1978. risk factors have analysed previous studies; role free-ranging pigs persistence suggested, but...
ABSTRACT Milk fat globules (MFGs) are vesicles released in milk as droplets surrounded by the endoplasmic reticulum and apical cell membranes. During formation apocrine secretion lactocytes, various amounts of cytoplasmic crescents remain trapped within vesicle, making MFGs a natural sampling mechanism lactating contents. With aim investigating events occurring mammary epithelium during bacterial infection, MFG proteome was characterized two-dimensional difference gel electrophoresis (2-D...
Cervical cancer ranks as the first most frequent among women in Benin. The major cause of cervical now recognized is persistent infection Human Papillomavirus (HPV). In Benin there a lack screening programs for prevention and little information exists regarding HPV genotype distribution.Cervical cells from 725 were examined presence viral DNA by means polymerase chain reaction (PCR) multiplex-based assay with amplification fragment L1 region E6/E7 genome, abnormal cytology Papanicolaou...
Neutrophil granulocytes are paramount to innate responses as major effectors of acute inflammation. Among the various strategies enacted by neutrophils eliminate microbes NETosis is a novel distinct antimicrobial activity in which an interlacement chromatin fibres rich granule-derived peptides and enzymes extruded (NETs, extracellular traps ). NETs contribute pathogenesis chronic inflammatory disorders. The interactions mycoplasmas immune cells, particular neutrophil granulocytes, poorly...
Abstract Mycoplasma lipoproteins play a relevant role in pathogenicity and directly interact with the host immune system. Among human mycoplasmas, hominis is described as commensal bacterium that can be associated number of genital extragenital conditions. Mechanisms M. are still largely obscure, only limited proteins have been virulence. The current study focused on investigating MHO_0730 virulence factor demonstrated surface lipoprotein, potentially expressed vivo during natural infection,...
An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves a managed herd. No Moraxella spp. or bacteria other than Mycoplasma were obtained from conjunctival nasal swabs. A strategy designed for characterization bovine mycoplasmas at species strain level on the basis combination molecular tools immunoblotting method. The made it possible to rapidly assign bacterium responsible this outbreak one phylogenetic clusters...
Bats may be natural reservoirs for a large variety of emerging viruses, including mammalian coronaviruses (CoV). The recent emergence severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and Middle East syndrome (MERS-CoV) in humans, with evidence that these viruses have their ancestry bats, highlights the importance virus surveillance bat populations. Here, we report identification molecular characterization β-Coronavirus, detected during viral survey carried out on different...
Gastrointestinal disorders caused by enteric viruses are frequently reported in dogs worldwide, with significant mortality rates unvaccinated individuals. This study reports the identification and molecular characterization of Canine parvovirus (CPV-2), coronavirus (CcoV), astrovirus (AstV), calicivirus (CcaV) a panel showing severe clinical signs sampled typical Mediterranean environment (Sardinia, Italy). At least one these viral species was detected 92.3% samples. CPV-2 most virus...
Free-living and captive chelonians might suffer from upper respiratory tract disease (URTD), a pathology primarily caused by Mycoplasma agassizii. Wild tortoises can also be an important reservoir of Salmonella spp., which are commensal in the host reptile but potential zoonotic agents. Between July 2009 June 2010, we screened free-living European (spur-thighed Testudo graeca, Hermann's hermanni, marginated marginata) temporarily housed wildlife center Italy. We molecularly characterized 13...
ABSTRACT A major surface antigenic lipoprotein of Mycoplasma agalactiae , promptly recognized by the host's immune system, was characterized. The mature product, P48, showed significant similarity and shared conserved amino acid motifs with lipoproteins or predicted from fermentans hyorhinis relapsing fever Borrelia spp., Bacillus subtilis Treponema pallidum .
Abstract Background Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with loss many biosynthetic pathways and cell wall. In this work, liposoluble protein complement Mycoplasma agalactiae , a minimal bacterial pathogen causing mastitis, polyarthritis, keratoconjunctivitis, abortion in small ruminants, was subjected to systematic characterization order gain insights into its membrane proteome composition. Results...
In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to staphylococcal SNase was characterized and its antigenicity during natural infections established. A UGA corrected version lacking region encoding signal peptide, expressed in Escherichia coli as GST fusion protein. Recombinant GST-MAG_5040 exhibits similar typical sugar-nonspecific endo- exonucleases, with DNA preferred substrate optimal presence 20 mM MgCl2 at temperatures...