Abstract With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective editors. A key factor facilitating efficient CRISPR delivery into cells, which known to be associated with size of system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among Un1Cas12f1 2.6 times smaller than SpCas9, providing advantages therapy research. Despite extensive engineering efforts improve Un1Cas12f1, efficiency still...

ABSTRACT: Effect of gamma‐irradiation on color, pungency, and volatiles Korean red pepper powder ( Capsicum annuum L.) was investigated. Red powder, vacuum‐packaged in a polyethylene/polypropylene bag, gamma‐irradiated up to 7 kGy. An irradiation dose kGy reduced the population mesophilic bacteria fungi effectively without affecting major quality factors. Pungency irradiated not changed based amount capsanoids by high‐performance liquid chromatography (HPLC) Scoville sensory score. The color...

The anti-atherogenic effects of spirulina (Spirulina platensis) were investigated in the New Zealand White (NZW) rabbit model. animal had hypercholesterolemia induced by being fed a high cholesterol diet (HCD) containing 0.5% for 4 wk, and then HCD supplemented with 1 or 5% (SP1 SP5) an additional 8 wk. Spirulina supplementation lowered intimal surface aorta 32.2 to 48.3%, compared HCD. Serum triglyceride (TG) total (TC) significantly reduced SP groups. After serum low density lipoprotein...

Abstract Lipase‐catalyzed interesterified solid fat was produced with fully hydrogenated soybean oil (FHSBO), and rapeseed (RSO) palm stearin (PS) in a weight ratio of 15:20:65, 15:40:45 15:50:35. The fats contained palmitic (27.8–44.6%), stearic (15.6–16.2%), oleic (27.5–36.5%) linoleic acids (8.0–13.5%). After interesterification the blends, physical properties products changed showed lower melting points contents, different crystallization behaviors as well formation more stable crystals....

The highly polymorphic swine leucocyte antigen (SLA) genes are one of the most important determinants in immune responses to infectious diseases, vaccines, and transplantation success. Study SLA influence requires accurate effective typing methods. We developed a simple rapid method type alleles at three classical class I loci (SLA-1, SLA-3 SLA-2) using PCR-sequence-specific primer (PCR-SSP) strategy. This system relies on 47 discriminatory PCR pairs designed amplify by groups that have...

Abstract BACKGROUND: Trans ‐free interesterified fat was produced for possible usage as a spreadable margarine stock. Rice bran oil, palm stearin and coconut oil were used substrates lipase‐catalyzed reaction. RESULTS: After interesterification, 137–150 g kg −1 medium‐chain fatty acid incorporated into the triacylglycerol (TAG) of fats. Solid contents at 25 °C 15.5–34.2%, slip melting point ranged from 27.5 to 34.3 °C. POP PPP (β‐tending TAG) in decreased after interesterification. X‐ray...

The hepatoprotective effect of aged black garlic (ABG) against ethanol-induced oxidative liver damage was investigated in adult male Sprague–Dawley rats for 4 weeks. Rats were divided into three groups: a saline (WT) group, an ethanol (ET) group (15 mL/kg body weight 20% [wt/vol] ethanol), and + ABG (ET+ABG) (ethanol 100 mg/kg ABG). administration led to decreased epididymal total fat pad (P<.05) weights, ameliorated prominent fatty changes around the portal triad, reduced accumulation...

The physicochemical characteristics and textural properties of yellow croaker treated by hot air drying (HAD), low temperature vacuum (LVD), freeze (FD) methods were studied. dried fish LVD had the lowest moisture content highest protein. volatile basic nitrogen values HAD, LVD, FD 66.27, 34.38, 33.03 mg/100 g sample, respectively. predominant amino acids lysine, taurine, alanine, glutamic acid, ones HAD remaining analyzed in this study, except acid. By using color parameters, L* b* showed...

The effect of diet supplemented with red beet (Beta vulgaris L.) leaf on antioxidant status plasma and tissue was investigated in C57BL/6J mice. mice were randomly divided into two groups after one-week acclimation, fed a high fat (20%) cholesterol (1%) without (control group) or 8% freeze-dried (RBL for 4 weeks. In RBL mice, lipid peroxidation determined as 2-thiobarbituric acid-reactive substances (TBARS value) significantly reduced the selected organs (liver, heart, kidney). Levels...

Background Hair greying is an obvious sign of ageing in humans. White (nonpigmented) hair thicker than black (pigmented) hair. The growth rate white also significantly higher that However, the mechanism underlying this largely unknown. Objectives To examine association between and patterns by evaluating expression genes or proteins related to hairs. Methods Morphological characteristics were observed eyebrow scalp differential was analysed hairs from human a microarray analysis. Reverse...

A ridge waveguide quantum dot (QD) laser with a stripe width of 15 μm was fabricated by using the seven-stacked InAs QD layers based on InAlGaAs-InAlAs material system InP [001] substrate. Room-temperature lasing operation observed at 1.501 μm, which is first observation from QDs structure. The characteristic temperature calculated dependence threshold current density 135 K in range 200 to room temperature.

Structured lipid (SL) was synthesized from extravirgin olive oil (EVOO) and conjugated linoleic acid (CLA) via a lipase-catalyzed reaction. CLA provides variety of health benefits, but it is not consumed in free fatty form. The SL contained 42.5 mol % isomers, the major isomers were cis-9,trans-11-CLA (16.9 %) trans-10,cis-12-CLA (24.2 %). antioxidant activity determined by radical scavenging capacity with 2,2-diphenyl-1-picrylhydrazyl lower than EVOO. oxidative stability also since had...

Interesterified plastic fats were produced with trans-free substrates of fully hydrogenated soybean oil, extra virgin olive and palm stearin in a weight ratio 10:20:70, 10:40:50, 10:50:40, respectively, by lipase catalysis. The major fatty acids the products palmitic (32.2−47.4%), stearic (12.0−12.4%), oleic acid (33.6−49.5%). After storage at 5 °C (refrigerator temperature) or 24 (room for 16 h, physical properties evaluated solid fat content, texture, melting, crystallization behavior,...

Rice bran oil (RBO) was fractionated into 2 phases, solid (S-RBO) and liquid (L-RBO), using acetone at -18 degrees C the weight yield of each S-RBO L-RBO 45.5% 54.5%, respectively. Then, trans-free hard fat synthesized from substrate fully hydrogenated soybean (FHSBO) different molar ratios (S-RBO : FHSBO; 1 1, 1.5, 2, 3) with Lipozyme TL IM lipase (10% total substrate). Conjugated linoleic acid (CLA, 20% substrate) used as functional fatty acids for production fat. After analysis, CLA...

The beneficial effect of Spirulina (Spirulina platensis) on tissue lipid peroxidation and oxidative DNA damage was tested in the hypercholesterolemic New Zealand White rabbit model. After hypercholesterolemia induced by feeding a high cholesterol (0.5%) diet (HCD) for 4 weeks, then HCD supplemented with 1% or 5% (SP1 SP5, respectively) provided an additional 8 weeks. supplementation significantly reduced increased level HCD-fed rabbits, levels recovered to control values. Oxidative stress...

Conjugated linoleic acids (CLA) were enzymatically acidolyzed with olive oil to produce structured lipids (SL), and their antiatherosclerotic properties investigated in C57BL/6J mice. Twenty-eight mice divided into four groups fed control diet or atherogenic diets supplemented high cholesterol fat (HCHF) containing 5% of lard, oil, SL based on for 4 weeks. The supplementation (0.6% CLA) significantly reduced the levels serum total triglyceride increased high-density lipoprotein level as...

ABSTRACT The equilibrium moisture content of raw and blanched almonds California grown “Carmel” variety was determined by using static gravimetric method at 25, 35 45C over a range water activities ( A w ) from 0.11 to 0.97. sorption data were fitted the Guggenheim–Anderson–de Boer (GAB) model. goodness fit model evaluated standard error estimate, mean relative percentage deviation modulus randomness residual, which showed good GAB equation experimental data. adsorption isotherms exhibited...