A5021628951- CRISPR and Genetic Engineering
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Circadian rhythm and melatonin
- Dietary Effects on Health
- Genetics and Neurodevelopmental Disorders
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Psychological and Temporal Perspectives Research
Korea University
2020-2025
Seoul National University
2025
Abstract With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective editors. A key factor facilitating efficient CRISPR delivery into cells, which known to be associated with size of system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among Un1Cas12f1 2.6 times smaller than SpCas9, providing advantages therapy research. Despite extensive engineering efforts improve Un1Cas12f1, efficiency still...
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize improve the efficiency prime-editing. Herein, we introduce two strategies editing using proximal dead sgRNA chromatin-modulating peptides. We used enhanced prime-editing generate Igf2 mutant mice with frequencies up 47% observed germline transmission, no off-target effects, dwarf phenotype....
The CRISPR-Cas system stands out as a promising genome editing tool due to its cost-effectiveness and time efficiency compared other methods. This has tremendous potential for treating various diseases, including genetic disorders cancer, promotes therapeutic research wide range of diseases. Additionally, the simplifies generation animal models, offering more accessible alternative traditional CRISPR-Cas9 can be used cleave target DNA strands that need corrected, causing double-strand breaks...
Abstract Genetically engineered mouse models (GEMMs) are crucial for investigating disease mechanisms, developing therapeutic strategies, and advancing fundamental biological research. While CRISPR gene editing has greatly facilitated the creation of these models, existing techniques still present technical challenges efficiency limitations. Here, we establish a CRISPR-VLP-induced targeted mutagenesis (CRISPR-VIM) strategy, enabling precise genome by co-culturing zygotes with virus-like...
Mucin 1 (MUC1) is a transmembrane glycoprotein involved in tumorigenesis of diverse cancers. However, the role MUC1 glioblastoma (GBM) has not yet been fully explored. In this study, anticancer mechanism suppression GBM was investigated. The expression level analyzed human glioma and paired normal brain tissues. overexpressed negatively associated with overall survival. Moreover, we silenced to investigate its effect cell lines found that knockdown inhibited proliferation resulted cycle...
Base editors are powerful tools for making precise single-nucleotide changes in the genome. However, they can lead to unintended insertions and deletions at target sites, which is a significant limitation clinical applications. In this study, we aimed eliminate unwanted indels sites caused by various evolved base editors. Accordingly, applied dead Cas9 instead of nickase induce accurate substitutions without indels. Additionally, tested use chromatin-modulating peptides improve nucleotide...
Glioblastomas (GBM) exhibit intratumoral heterogeneity of various oncogenic evolutional processes. We have successfully isolated and established two distinct cancer cell lines with different morphological biological characteristics that were derived from the same tissue sample a GBM. When we compared their genomic transcriptomic characteristics, each line harbored mutation clusters while sharing core driver mutations. Transcriptomic analysis revealed one was undergoing mesenchymal transition...