A5057807816- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Regulation of Appetite and Obesity
- Neurogenesis and neuroplasticity mechanisms
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Autophagy in Disease and Therapy
- Diet and metabolism studies
- Neonatal Respiratory Health Research
- Peroxisome Proliferator-Activated Receptors
- Hepatitis B Virus Studies
- Microtubule and mitosis dynamics
- COVID-19 Clinical Research Studies
- Cancer-related gene regulation
- Photosynthetic Processes and Mechanisms
- Cancer Genomics and Diagnostics
- Biochemical effects in animals
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- Endoplasmic Reticulum Stress and Disease
Seoul National University
2016-2025
Korea Disease Control and Prevention Agency
2025
Korea National Institute of Health
2025
Gyeongsang National University
2023
New Generation University College
2005-2022
Yonsei University
2005-2021
Creative Research
2018
In-Q-Tel
2002-2018
Seoul Institute
2018
Centre Oscar Lambret
2018
Rationale: Monocyte infiltration into the subintimal space and its intracellular lipid accumulation are most prominent features of atherosclerosis. To understand pathophysiology atherosclerotic disease, we need to characteristics lipid-laden foamy macrophages in during Objective: We sought examine transcriptomic profiles nonfoamy isolated from intima. Methods Results: Single-cell RNA sequencing analysis CD45 + leukocytes murine aorta revealed that there macrophage subpopulations with...
Firmicutes and Bacteroidetes, 2 major phyla of gut microbiota, are involved in lipid bile acid metabolism to maintain systemic energy homeostasis host. Recently, accumulating evidence has suggested that dietary changes promptly induce the alteration abundance both Bacteroidetes obesity its related metabolic diseases. Nevertheless, roles on such disease states remain unclear. The aim this study was determine effects antibiotic-induced depletion dysregulation obesity. Treatment C57BL/6J mice...
Single-cell transcriptomic profiles analysis has proposed new insights for understanding the behavior of human gastric cancer (GC). GC offers a unique model intratumoral heterogeneity. However, specific classes cells involved in carcinogenetic passage, and tumor microenvironment stromal was poorly understood. We characterized heterogeneous cell population precancerous lesions at single-cell resolution by RNA sequencing. identified 10 subtypes showed intestinal diffuse-type were different...
Abstract Recent evidence suggests that obese animals exhibit increased endoplasmic reticulum (ER) stress in the liver and adipose tissue. Although ER is closely associated with lipid homeostasis, it largely unknown how contributes to hepatic steatosis. In this study, we demonstrate induction of stimulates steatosis through expression very low-density lipoprotein receptor (VLDLR). Among unfolded protein response sensors, kinase RNA-like kinase–activating transcription factor 4 signaling...
Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in lipid accumulation. We found that the C3H mouse strain does not express PPARγ liver and, when subject to a high-fat diet, is resistant steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 injection into B6 and mice caused microarray analysis demonstrated expression associated genes involved fatty acid transport triglyceride synthesis pathway. In particular, significantly increased of...
Abstract Although hepatitis B virus X protein (HBx) has been implicated in abnormal lipid metabolism (HBV)–associated hepatic steatosis, its underlying molecular mechanism remains unclear. Liver receptor (LXR) plays an important role regulating the expression of genes involved lipogenesis. Here we demonstrate that LXRα and LXRβ mediate HBV-associated steatosis. We have found HBx induces LXR lipogenic target genes, such as sterol regulatory element binding protein-1c (SREBP-1c), fatty acid...
Abstract The retinoic acid receptor-related orphan receptor-α (RORα) is an important regulator of various biological processes, including cerebellum development, circadian rhythm and cancer. Here, we show that hepatic RORα controls lipid homeostasis by negatively regulating transcriptional activity peroxisome proliferators-activated receptor-γ (PPARγ) mediates metabolism. Liver-specific Rorα -deficient mice develop steatosis, obesity insulin resistance when challenged with a high-fat diet...
Patient-derived tumor xenograft (PDX) mouse models have emerged as an important oncology research platform to study evolution, mechanisms of drug response and resistance, tailoring chemotherapeutic approaches for individual patients. The lack robust standards reporting on PDX has hampered the ability researchers find relevant associated data. Here we present minimal information standard (PDX-MI) generation, quality assurance, use models. PDX-MI defines describing clinical attributes a...
We provide detailed mechanisms of Ahnak-mediated potentiation transforming growth factor β (TGFβ) signaling, which leads to a negative regulation cell growth. show that Smad3 interacts with Ahnak through MH2 domain and stimulates localization into nucleus leading potentiating TGFβ-induced transcriptional activity R-Smad. Moreover, overexpression resulted in retardation cycle arrest downregulation c-Myc cyclin D1/D2. describe results from analyses Ahnak−/− mouse model expressing middle T...
Abstract The International Mouse Phenotyping Consortium (IMPC) is building a catalogue of mammalian gene function by producing and phenotyping knockout mouse line for every protein-coding gene. To date, the IMPC has generated characterised 5186 mutant lines. One-third lines have been found to be non-viable over 300 new models human disease identified thus far. While current bioinformatics efforts are focused on translating results better understand processes, data also aids understanding...
Protein arginine methyltransferases (PRMTs) have emerged as important regulators of skeletal muscle metabolism and regeneration. However, the direct roles various PRMTs during remodeling remain unclear. Using muscle-specific prmt1 knockout mice, we examined function downstream targets PRMT1 in homeostasis. We found that deficiency led to atrophy. PRMT1-deficient muscles exhibited enhanced expression a macroautophagic/autophagic marker LC3-II, FOXO3 ubiquitin ligases, TRIM63/MURF-1 FBXO32,...
The higher prevalence of obesity-related metabolic disease in males suggests that female sex hormones provide protective mechanisms against the pathogenesis syndrome. Because browning white adipose tissue (WAT) is disease, we examined differences β3-adrenergic remodeling WAT mice.Effects receptor agonist CL316,243 (CL) on were investigated male and C57BL mice. role ovarian female-specific was studied control mice with failure induced by 4-vinylcyclohexene diepoxide treatment for 15 days.We...
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize improve the efficiency prime-editing. Herein, we introduce two strategies editing using proximal dead sgRNA chromatin-modulating peptides. We used enhanced prime-editing generate Igf2 mutant mice with frequencies up 47% observed germline transmission, no off-target effects, dwarf phenotype....
Abstract Background Aging is a natural process that an organism gradually loses its physical fitness and functionality. Great efforts have been made to understand intervene in this deteriorating process. The gut microbiota affects host physiology, dysbiosis of the microbial community often underlies pathogenesis disorders. commensal also changes with aging; however, interplay between aging remains largely unexplored. Here, we systematically examined ameliorating effects derived from young on...
Hepatitis B virus X protein (HBx) of the hepatitis was strongly implicated in angiogenesis and metastasis during hepatocarcinogenesis. Here, we explored possibility cross-talk between HBx hypoxia-inducible factor-1alpha (HIF-1alpha), a potent transcriptional inducer angiogenic factors. First, showed that stability HIF-1alpha increased by HBx-inducible Chang liver cells as well transient expression system non-hepatic cells. Immunofluorescence studies revealed HBx-induced partially...
beta-Catenin regulates important biological processes, including embryonic development and tumorigenesis. We have investigated the role of beta-catenin in regulation chondrocyte phenotype. Expression was high prechondrogenic mesenchymal cells, but significantly decreased differentiated chondrocytes both vivo vitro. Accumulation by inhibition glycogen synthase kinase-3beta with LiCl inhibited chondrogenesis stabilizing cell-cell adhesion. Conversely, low level articular increased...
Tamoxifen is commonly used to treat patients with ESR/ER-positive breast cancer, but its therapeutic benefit limited by the development of resistance. Recently, alterations in macroautophagy/autophagy function were demonstrated be a potential mechanism for tamoxifen Although MTA1 (metastasis-associated 1) has been implicated tumorigenesis and metastasis, role endocrine resistance not studied. Here, we report that level expression was upregulated resistant cancer cell lines MCF7/TAMR T47D/TR,...
The majority of immune cells in the brain are comprised microglia, which undergo morphological changes when activated to remove damaged neurons and infectious agents from tissue. In this study, we investigated effects type 2 diabetes on microglial activation subsequent secretion pro-inflammatory cytokines, such as interferon-gamma (IFN-gamma) interleukin-1beta (IL-1beta), hippocampus using Zucker diabetic fatty (ZDF) rats lean control (ZLC) at various stages.Zucker were sacrificed 12 (early...
Metastasis-associated protein 1 (MTA1) is a component of the nucleosome remodeling and histone deacetylase (HDAC) complex, which plays an important role in progression breast cancer. Although MTA1 known as repressor transactivation function estrogen receptor α (ERα), its involvement epigenetic control transcription ERα gene ESR1 has not been studied. Here, we show that silencing reduced level expression ERα-positive cells but increased it ERα-negative cells. In both MCF7 MDA-MB-231, was...
AMP-activated protein kinase (AMPK) plays a key role in controlling energy metabolism response to physiological and nutritional status. Although AMPK activation has been proposed as promising molecular target for treating obesity its related comorbidities, the use of pharmacological activators met with contradictory therapeutic challenges. Here we show regulatory mechanism through ubiquitination degradation by E3 ubiquitin ligase makorin ring finger 1 (MKRN1). MKRN1 depletion promotes...