A5019900739- Peroxisome Proliferator-Activated Receptors
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Advanced Manufacturing and Logistics Optimization
- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Cholesterol and Lipid Metabolism
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- Cancer-related Molecular Pathways
- Kruppel-like factors research
- Adipokines, Inflammation, and Metabolic Diseases
- Liver Disease Diagnosis and Treatment
- Cardiovascular Function and Risk Factors
- Scheduling and Optimization Algorithms
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- FOXO transcription factor regulation
- Assembly Line Balancing Optimization
- Sirtuins and Resveratrol in Medicine
- Drug Transport and Resistance Mechanisms
- Cardiac Valve Diseases and Treatments
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
Yonsei University
2015-2025
Gangnam Severance Hospital
2019
Daegu Haany University
2019
Catholic University of Daegu
2019
Sri Venkateswara Institute of Medical Sciences
2018
Korea Research Institute of Bioscience and Biotechnology
2017
Severance Hospital
2016
Korea Advanced Institute of Science and Technology
2016
Hankuk University of Foreign Studies
2015
Seoul National University Bundang Hospital
2015
Since the use of magnetic nanocrystals as probes for biomedical system is attractive, it important to develop optimal synthetic protocols high-quality and have systematic understanding their nanoscale properties. Here we present development a synthetically controlled nanocrystal model that correlates tunabilities in terms size, magnetism, induced nuclear spin relaxation processes. This further led high-performance nanocrystal−antibody probe systems diagnosis breast cancer cells via resonance imaging.
The unique properties of magnetic nanocrystals provide them with high potential as key probes and vectors in the next generation biomedical applications. Although superparamagnetic iron oxide have been extensively studied excellent resonance imaging (MRI) for various cell trafficking, gene expression, cancer diagnosis, further development vivo MRI applications has very limited. Here, we describe diagnosis cancer, utilizing a well-defined nanocrystal probe system multiple capabilities, such...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a member of the subtilases that promotes internalization and degradation LDL receptor in liver thereby controls level cholesterol plasma. Here, we show expression PCSK9 HepG2 cells completely dependent on absence or presence sterols. The minimal promoter region gene contains sterol-regulatory element (SRE), which makes transcription Expression nuclear forms binding protein-1 (SREBP-1) SREBP-2 dramatically increased activity PCSK9. In...
Leptin, the product of ob gene, controls food-intake and weight loss in mouse. Although target(s) circulating leptin is presumed to be brain which then orchestrates loss, how functions process unknown. In this report, we present evidence that gene expression cultured cells suppresses acetyl-CoA carboxylase lipid synthesis are induced by hormone treatment. This first example has been found suppress defined biochemical reactions contribute accumulation without participation brain.
Expression of the HER2 oncogene is increased in approximately 30% human breast carcinomas and closely correlated with expression fatty acid synthase (FASN). In present study, we determined mechanism by which FASN acetyl-CoA carboxylase alpha (ACCalpha) could be induced overexpression. SK-BR-3 BT-474 cells, cancer cells that overexpress HER2, expressed higher levels ACCalpha compared MCF-7 MDA-MB-231 low. The induction BT474 were not mediated activation SREBP-1. Exogenous ACCalpha,...
Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in lipid accumulation. We found that the C3H mouse strain does not express PPARγ liver and, when subject to a high-fat diet, is resistant steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 injection into B6 and mice caused microarray analysis demonstrated expression associated genes involved fatty acid transport triglyceride synthesis pathway. In particular, significantly increased of...
Lipin1 expression was induced at a late stage of differentiation 3T3-L1 preadipocytes and maintained high levels in mature adipocytes. Knockdown lipin1 by small interfering RNA almost completely inhibited into adipocytes, whereas overexpression accelerated adipocyte differentiation, demonstrating that is required for differentiation. In transfection lipin1-small decreased the functional genes, indicating involvement maintenance function. increases transcription-activating function peroxisome...
GLUT2 is mainly expressed in the liver, β-cells of pancreas, and basolateral membrane kidney proximal tubules plays an important role glucose homeostasis living organisms. The transcription gene known to be upregulated liver during postprandial hyperglycemic states or type 2 diabetes. However, a molecular mechanism by which activates expression not known. In this study, we report evidence that sterol response element–binding protein (SREBP)-1c key glucose-stimulated expression. promoter...
Up-regulation of lipogenesis by androgen is one the most characteristic metabolic features LNCaP prostate cancer cells. The present study revealed that increases glucose utilization for de novo in cells through activation HK2 (hexokinase 2) and cardiac isoform PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase). Activation PKA (cAMP-dependent protein kinase) increased phosphorylation CREB [CRE (cAMP-response element)-binding protein], which turn bound to CRE on promoter gene...
A retrospective review of 75 burst fractures the spine was performed to define radiographic features found on high-resolution CT, polytomography, and plain radiography thereby allow full characterization this uncommon spinal injury. Characteristic components injury include: centripetally oriented disruption vertebral body, unilateral or bilateral laminar that abut spinous process, marked anterior wedging, vertically fracture emanating from basivertebral foramen, increased interpediculate...
Krüppel-like factor 4 (KLF4) is a transcription that plays an important role in cell differentiation, proliferation, and survival, especially the context of cancers. This study revealed KLF4 activates glycolytic metabolism breast cancer cells by up-regulating platelet isoform phosphofructokinase (PFKP). activated PFKP gene directly binding to promoter. Whereas glucose uptake lactate production were inhibited knockdown KLF4, they overexpression KLF4. Unlike PFKP, expressions other isoforms...
We analyzed the pattern of <sup>11</sup>C-acetate and <sup>18</sup>F-FDG uptake on PET/CT in patients with hepatocellular carcinoma (HCC). also assessed expression important regulatory enzymes related to glycolysis lipid synthesis relation human HCC cell lines. The significance regulation was further evaluated regard viability. <b>Methods:</b><sup>18</sup>F-FDG patterns 11 5 lines were assessed. gene metabolic a line highest another uptake. They included hexokinase II, adenosine triphosphate...
Deprivation of tumor bioenergetics by inhibition multiple energy pathways has been suggested as an effective therapeutic approach for various human tumors. However, this idea not evaluated in glioblastoma (GBM). We hypothesized that dual glycolysis and oxidative phosphorylation could effectively suppress GBM tumorspheres (TS). Effects 2-deoxyglucose (2DG) metformin, alone combination, on GBM-TS were evaluated. Viability, cellular metabolism status, stemness, invasive properties,...
We identified the peroxisomal proliferator response element (PPRE) in +68/+89 region of rat GLUT2 gene. To identify whether putative PPRE gene (GLUT2-PPRE) is functional, promoter-luciferase reporter constructs were transfected into CV-1 cells. Promoter activities increased by coexpression proliferator-activated receptor (PPAR)-gamma, retinoid X (RXR)-alpha, and treatment their ligands; troglitazone 9-cis retinoic acid potentiated transactivational effects. Introduction mutations GLUT2-PPRE...
Adiponectin is one of the adipokines secreted by adipocytes and regulates energy homeostasis associated with insulin sensitivity, suggesting a possibility nutritional regulation adiponectin gene expression. In this study, we showed that transcription was induced 4–6 h after refeeding mice. Also, differentiated 3T3-L1 were treated high glucose expressed significantly increased mRNA. Promoter analysis using nuclear extracts from white adipose tissue revealed CCAAT/enhancer binding protein...
The regulation of hepatic glucose metabolism is important in homeostasis, and liver glucokinase (LGK) plays a central role this process. Hepatic expression known to be regulated by insulin. Recently it has been suggested that sterol regulatory element binding protein-1c (SREBP-1c) mediates the action insulin on LGK transcription; however, precise mechanism not, date, well known. In present study, we identified two functional SREBP-1c response elements, SREa SREb, rat promoter. could bind...
Liver glucokinase (LGK) plays an essential role in controlling blood glucose levels and maintaining cellular metabolic functions. Expression of LGK is induced mainly regulated by insulin through sterol regulatory element-binding protein-1c (SREBP-1c) as a mediator. Since expression known to be decreased the liver X receptor (LXR) knockout mice, we have investigated whether might directly activated LXRα. Furthermore, studied interrelationship between transcription factors that control gene...
FBI-1 (Pokemon/ZBTB7A) is a proto-oncogenic transcription factor of the BTB/POZ (bric-à-brac, tramtrack, and broad complex pox virus zinc finger) domain family. Recent evidence suggested that might be involved in adipogenic gene expression. Coincidentally, expression fatty-acid synthase (FASN) genes are often increased cancer immortalized cells. Both FASN important cell proliferation. SREBP-1 major regulator many genes, (sterol-responsive element (SRE)-binding protein 1) interact with each...
Abstract Background Most cancer cells employ the Warburg effect to support anabolic growth and tumorigenesis. Here, we discovered a key link between aberrantly activated Wnt/β-catenin signalling, especially by pathologically significant APC loss, in CRC. Methods Proteomic analyses were performed evaluate global effects of KYA1797K, signalling inhibitor, on cellular proteins The -loss or Wnt ligand identified enzymes, PKM2 LDHA, as well investigated. A linkage activation metabolism was...