A5047885323- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Toxic Organic Pollutants Impact
- Metabolism and Genetic Disorders
- Effects and risks of endocrine disrupting chemicals
- Peroxisome Proliferator-Activated Receptors
- Neuroinflammation and Neurodegeneration Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Birth, Development, and Health
- Pancreatic function and diabetes
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Coronary Interventions and Diagnostics
- Nuclear Receptors and Signaling
- RNA modifications and cancer
Kyung Hee University
2015-2024
Kyung Hee University Medical Center
2021
University of Ulsan
2003-2007
Ulsan College
2004-2007
Ulsan University Hospital
2005
Asan Medical Center
2005
Seoul National University
2005
Chonnam National University
2005
Kangwon National University
2003
Korea National Institute of Health
1999-2002
Lipopolysaccharide (LPS) increases the production of interleukin-12 (IL-12) from mouse macrophages via a κB site within IL-12 p40 promoter. In this study, we found that oxidized low density lipoprotein (oxLDL) inhibited LPS-stimulated in dose-dependent manner while native LDL did not. OxLDL promoter activation monocytic RAW264.7 cells transiently transfected with promoter/reporter constructs, and repressive effect mapped to region containing binding for nuclear factor-κB (NF-κB) (p40-κB)....
There is an apparent overlap between areas in the USA where herbicide, atrazine (ATZ), heavily used and obesity-prevalence maps of people with a BMI over 30. Given that herbicides act on photosystem II thylakoid membrane chloroplasts, which have functional structure similar to mitochondria, we investigated whether chronic exposure low concentrations ATZ might cause obesity or insulin resistance by damaging mitochondrial function. Sprague-Dawley rats (n = 48) were treated for 5 months (30 300...
Ghrelin, a stomach-derived hormone, induces adiposity when administered to rodents. Because ghrelin receptor is abundantly expressed in adipose tissue, we investigated the role of adipocyte biology. We observed expression 3T3-L1 preadipocytes and adipocytes. Treatment with induced cellular proliferation differentiation mature adipocytes, as well basal insulin-stimulated glucose transport, but it inhibited apoptosis by serum deprivation. Exposure cells caused rapid activation MAPKs,...
OBJECTIVE—To investigate whether the peripheral blood mtDNA (pb-mtDNA) content is decreased and linked to insulin resistance in offspring of type 2 diabetic patients. RESEARCH DESIGN AND METHODS—A total 82 patients 52 age-, sex-, BMI-matched normal subjects from Mokdong, Korea, population were selected for this study by stratified, randomized sampling. Of patients, had glucose tolerance (NGT), 21 impaired (IGT), 9 newly diagnosed diabetes. The pb-mtDNA was measured real-time polymerase chain...
Depletion of mtDNA in myocytes causes insulin resistance and alters nuclear gene expression that may be involved rescuing processes against cellular stress. Here we show the C1q tumor necrosis factor alpha-related protein isoform 5 (C1QTNF5) is drastically increased following depletion myocytes. C1QTNF5 homologous to adiponectin respect domain structure, its secretion from correlated negatively with content. Similar adiponectin, induced phosphorylation AMP-activated kinase (AMPK), leading...
Human umbilical cord mesenchymal stromal cells (hUC-MSCs) of Wharton's jelly origin undergo adipogenic, osteogenic, and chondrogenic differentiation in vitro. Recent studies have consistently shown their therapeutic potential various human disease models. However, the biological effects major pregnancy complications on cellular properties hUC-MSCs remain to be studied. In this study, we compared basic obtained from gestational diabetes mellitus (GDM) patients (GDM-UC-MSCs) normal pregnant...
An excess of reactive oxygen species (ROS) relative to the antioxidant capacity causes oxidative stress, which plays a role in development Parkinson's disease (PD). Because mitochondria are both sites ROS generation and targets damage, delivery antioxidants might prevent or alleviate PD. To transduce protein human metallothionein 1A (hMT1A) into mitochondria, we computationally designed cell-penetrating artificial mitochondria-targeting peptide (CAMP). The recombinant CAMP-conjugated hMT1A...
Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about link between and exposure to pollutants, including MPs. In this study, we conducted comprehensive investigation utilizing silico, vitro, vivo approaches explore brain distribution physiological effects of Molecular docking simulations were performed assess binding affinity...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT2D NMR and structural model for a mitochondrial signal peptide bound to micelleChristine Karslake, Martial E. Piotto, Youngmi K. Pak, Henry Weiner, David G. GorensteinCite this: Biochemistry 1990, 29, 42, 9872–9878Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://pubs.acs.org/doi/10.1021/bi00494a017https://doi.org/10.1021/bi00494a017research-articleACS PublicationsRequest...
This study examined whether ethyl pyruvate (EP) promotes the survival of nigrostriatal dopaminergic (DA) neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model Parkinson's disease. MPTP induced degeneration DA and glial activation as visualized by tyrosine hydroxylase, macrophage Ag complex-1, and/or fibrillary acidic protein immunoreactivity. Western blotting immunohistochemistry showed microglial NADPH oxidase astroglial myeloperoxidase (MPO) subsequent reactive oxygen...
Abstract Inflammation and oxidative stress play major roles in the pathogenesis after spinal cord injury (SCI). Here, we examined neuroprotective effects of Angelica dahuricae radix (ADR) extract SCI. ADR significantly decreased levels proinflammatory factors such as tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), inducible nitric oxide synthase (iNOS), cyclooxygenase‐2 (COX‐2) a lipopolysaccharide (LPS)‐activated microglial cell line, BV2 cells. also...
Transcription and replication of mitochondrial DNA (mtDNA) are regulated by nuclear DNA-encoded proteins that targeted into mitochondria. A decrease in mtDNA copy number results dysfunction, which may lead to insulin resistance metabolic syndromes. We analyzed physically bind human D-loop using a shot-gun proteomics approach following protein enrichment DNA-linked affinity chromatography. total 152 binding were identified peptide sequencing ultra high pressure capillary reverse-phase liquid...
Several environmental contaminants have been linked to the development of diabetes and increased diabetes‑associated mortality. Perfluorooctanoic acid (PFOA) is a widely used perfluoroalkane found in surfactants lubricants, processing aids production polymers. Furthermore, PFOA has detected humans, wildlife environment. The present study investigated toxic effects on rat pancreatic β‑cell‑derived RIN‑m5F cells. Cell viability, apoptosis, reactive oxygen nitrogen species, cytokine release...
Background— The migration of circulating monocytes to the arterial wall during atherogenesis is largely modulated by activation CC chemokine receptor 2 (CCR2), a dominant monocyte chemotaxis receptor. present study investigated whether 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibition affects CCR2 gene expression and CCR2-dependent recruitment. Methods Results— Competitive reverse transcription-polymerase chain reaction analysis flow cytometry showed that simvastatin, an...
In modern society, growing numbers of people are engaged in various forms shift works or trans-meridian travels. Such circadian misalignment is known to disturb endogenous diurnal rhythms, which may lead harmful physiological consequences including metabolic syndrome, obesity, cancer, cardiovascular disorders, and gastric disorders as well other physical mental disorders. However, the precise mechanism(s) underlying these changes yet unclear. The present work, therefore examined effects 6 h...
Serum concentrations of environmental pollutants have been positively correlated with diabetes and metabolic syndrome in epidemiologic studies. In turn, abnormal mitochondrial function has associated the diseases. The relationships between these variables, however, not studied. We developed novel cell‐based aryl hydrocarbon receptor (AhR) agonist bioassay system without solvent extraction process analyzed whether low‐dose circulating AhR ligands human serum are parameters function. ligand...