A5101751472- Microwave Engineering and Waveguides
- Electromagnetic Compatibility and Noise Suppression
- 3D IC and TSV technologies
- Radio Frequency Integrated Circuit Design
- Millimeter-Wave Propagation and Modeling
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- RNA Research and Splicing
- Semiconductor Lasers and Optical Devices
- Genomics and Chromatin Dynamics
- Wireless Power Transfer Systems
- Energy Harvesting in Wireless Networks
- Advanced Antenna and Metasurface Technologies
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Electrical and Thermal Properties of Materials
- Power Line Communications and Noise
- Ferroelectric and Piezoelectric Materials
- Microwave and Dielectric Measurement Techniques
- Nuclear Receptors and Signaling
- Photonic and Optical Devices
- Acoustic Wave Resonator Technologies
- Engineering Applied Research
- Antenna Design and Analysis
- Ubiquitin and proteasome pathways
Mokpo National Maritime University
2014-2024
Chonnam National University
2007-2021
Samsung (South Korea)
1998-2018
Korea Advanced Institute of Science and Technology
1990-2015
Seoul National University Hospital
2011-2012
Korea Food Research Institute
2003-2011
Mokpo National University
2010-2011
Kyungnam University
2002-2010
Telekom Malaysia Berhad (Malaysia)
2010
Diabetes Care & Hormone Clinic
2002
Many transcription coactivators interact with nuclear receptors in a ligand- and C-terminal transactivation function (AF2)-dependent manner. These include activating signal cointegrator 2 (ASC-2), recently isolated transcriptional coactivator molecule, which is amplified human cancers stimulates by numerous other factors. In this report, we show that ASC-2 belongs to steady-state complex of approximately MDa (ASC-2 [ASCOM]) HeLa nuclei. ASCOM contains retinoblastoma-binding protein RBQ-3,...
Many transcription coactivators interact with nuclear receptors in a ligand- and C-terminal transactivation function (AF2)-dependent manner. We isolated factor (designated ASC-2) such properties by using the ligand-binding domain of retinoid X receptor as bait yeast two-hybrid screening. ASC-2 also interacted other receptors, including retinoic acid receptor, thyroid hormone estrogen α, glucocorticoid basal factors TFIIA TBP, integrators CBP/p300 SRC-1. In transient cotransfections, ASC-2,...
The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription RNA polymerase II (PolII) composed two stable subcomplexes (Srb4 Rgr1 subcomplexes). To decipher the function each subcomplex to delineate functional relationship between subcomplexes, we characterized compositions biochemical activities PolII-Mediator complexes (holoenzymes) prepared from several mutant strains S. cerevisiae. We found that holoenzymes devoid a Gal11 module were...
The Mediator complex of Saccharomyces cerevisiae is required for diverse aspects transcription by RNA polymerase II (pol II). composed two functionally distinct subcomplexes, Rgr1 and Srb4. To identify the structures functions each subcomplex, we expressed recombinant proteins subunit assayed their interactions with other basal proteins. subcomplex Gal11 module, which binds activators, Med9/10 module. module both transcriptional activation repression, these activities appear to be carried...
Silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) is known to interact with Sin3 recruit the histone deacetylases (HDACs) that lead hypoacetylation histones transrepression target transcription factors. Herein, we found coexpression SMRT significantly repressed transactivations by activating protein-1 (AP-1), nuclear factor-κB (NFκB), serum response factor (SRF) in a dose-dependent manner, but not presence trichostatin A, specific inhibitor HDAC. Similarly, HDAC1...
There has been no unequivocal demonstration that the activator binding targets identified in vitro play a key role transcriptional activation vivo. To examine whether activator-Mediator interactions are required for gene transcription under physiological conditions, we performed functional analyses with Mediator components interact specifically natural yeast activators. Different activators via distinct targets. Deletion of region completely compromised vivo by some, but not all, These...
Human activating signal cointegrator 1 (hASC-1) was originally isolated as a transcriptional coactivator of nuclear receptors. Here we report that ASC-1 exists steady-state complex associated with three polypeptides, P200, P100, and P50, in HeLa nuclei; stimulates transactivation by serum response factor (SRF), protein (AP-1), κB (NF-κB) through direct binding to SRF, c-Jun, p50, p65; relieves the previously described transrepression between receptors either AP-1 or NF-κB. Interestingly,...
Regulated transcription of class II genes the yeastSaccharomyces cerevisiae requires diverse functions mediator complex. In particular, MED6 is essential for activated from many promoters, suggesting that it as a key player in relay activator signals to basal machinery. To identify functional relationship between and other transcriptional regulators, we conducted genetic screen isolate suppressor temperature-sensitive (ts) med6 mutation. We identified an SRB4 allele dominant allele-specific...
The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. composed two subcomplexes, the Rgr1 and Srb4 which appear to function in reception activator signals subsequent modulation Pol activity, respectively. In order determine precise composition explore specific role each protein, our goal was identify all components. To this end, we cloned three previously unidentified subunits, Med9/Cse2, Med10/Nut2, Med11, isolated...
In this paper, we present a wireless sensor system (WSS) that integrated an inductive power transfer (I-WPT) module for battery-free real-time monitoring of the status rotating shaft in ships. Firstly, optimized I-WPT seamless supply was implemented using multiple Tx and Rx coils, its capability 1.75 W with efficiency 75% achieved. Secondly, as result high-power performance module, entire WSS four sensors designed on rotary shaft. Finally, installed small-scale test bench diameter 200 mm; it...
Activating signal cointegrator 1 (ASC-1) harbors an autonomous transactivation domain that contains a putative zinc finger motif which provides binding sites for basal transcription factors TBP and TFIIA, integrators steroid receptor coactivator (SRC-1) CBP-p300, nuclear receptors, as demonstrated by the glutathione S-transferase pull-down assays yeast two-hybrid tests. The ASC-1 involve hinge but not C-terminal AF2 core of receptors. Nonetheless, appears to require AF2-dependent function...
Abstract Nuclear receptor (NR) transactivation involves multiple coactivators, and the molecular basis for how these are functionally integrated needs to be determined fully understand NR action. Activating signal cointegrator-2 (ASC-2), a transcriptional coactivator of many NRs transcription factors, forms steady-state complex, ASCOM (for ASC-2 complex), which contains histone H3-lysine-4 (H3K4) methyltransferase MLL3 or its paralog MLL4. Here, we show that requires functional cross talk...
Ser-5 phosphorylation of the RNA polymerase II (Pol II) C-terminal domain by TFIIH kinase has been implicated in critical steps mRNA synthesis, such as Pol promoter escape and 5'-capping. However, general requirement precise role transcription still remain elusive. Here we use a chemical genetics approach to show that, for majority budding-yeast genes, specific inhibition yeast results dramatic reduction both level phosphorylation. Surprisingly, activity only partially affected density Ser-2...
ASC-2 was recently discovered as a cancer-amplified transcription coactivator molecule of nuclear receptors, which interacts with multifunctional integrators steroid receptor coactivator-1 (SRC-1) and CREB-binding protein (CBP)/p300. Herein, we report the identification three mitogenic factors novel target molecules ASC-2. First, C-terminal transactivation domain serum response factor (SRF) identified among series ASC-2-interacting proteins from yeast two-hybrid screening. Second,...
We demonstrate a 36 times 12 0.9mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> sized compact monolithic LTCC SiP transmitter (Tx) for 60GHz-band wireless communication terminal applications. Five GaAs MMICs including mixer, driver amplifier, power amplifier and two of frequency doublers have been integrated onto multilayer circuit which embeds stripline BPF microstrip patch antenna. A novel CPW-to-stripline transition has devised...
Abstract In this article, a novel design for the modified bow‐tie slot antenna with rectangular tuning stub 2.4/5.2/5.8 GHz triple‐band applications is presented. The prototype consists of slot, strip line in and feeding. simulation results showed that impedance matching proposed was greatly affected by feed gap slot. experiment indicate bandwidth (return loss: −10 dB definition) about 1.405 2.4 band 1.305 5 band. radiation patterns gains are also presented discussed. © 2010 Wiley...
Modification of chromatin and related transcription factors by histone deacetylases (HDACs) is one the major strategies for controlling gene expression in eukaryotes. The HDAC domains class IIa HDACs repress respective target genes interacting with C-terminal region silencing mediator retinoid thyroid receptor (SMRT) repression domain 3 (SRD3c). However, latent catalytic activity suggests that their roles as regulation are unclear. Here, we found two conserved GSI-containing motifs SRD3c...
To repress the expression of target genes, unliganded nuclear receptor generally recruits silencing mediator retinoid and thyroid hormone (SMRT)/nuclear corepressor via its direct association with conserved motif within bipartite receptor-interaction domains (IDs) corepressor. Here, we investigated involvement SMRT in transcriptional repression by vitamin D (VDR). Using small interference RNA against human embryonic kidney 293 cells, demonstrated that is involved VDR-target osteocalcin D(3)...
In this work, CPW-to-stripline (SL) vertical via transitions using gradually stepped vias and embedded air cavities are presented for V-band LTCC System-on-Package (SoP) applications. order to reduce radiation loss due abrupt discontinuities, gradual proposed investigated. addition, in increased parasitic shunt capacitance structures, below the transition vias. Using a 3-D EM simulation tool, designed analyzed, compared conventional transition. Three-segment transmission lines (CPW-SL-CPW)...
The fate of stem cells is intricately regulated by numerous extrinsic and intrinsic factors that promote maintenance or differentiation. RNA-binding translational repressor Pumilio (Pum) in conjunction with Nanos (Nos) required for self-renewal, whereas Bam (bag-of-marbles) Bgcn (benign gonial cell neoplasm) differentiation germ line the Drosophila ovary. Genetic analysis suggests antagonize Pum/Nos function to differentiation; however, molecular basis this epistatic relationship currently...
In this paper, a novel LTCC stripline (SL) 60-GHz band-pass filter (BPF) composed of transitions to coplanar waveguide (CPW) pads for monolithic microwave integrated circuit integration is presented. For low-loss interconnection with active devices, CPW-to-SL vertical via transition integrating air cavities and CPW-to-CPW planar using internal ground planes are proposed implemented. The fabricated shows an insertion loss 1.6 dB reflection below -20 at the passband filter. implemented SL BPF...