A5000172274- Ubiquitin and proteasome pathways
- Molecular Sensors and Ion Detection
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Electrochemical Analysis and Applications
- RNA modifications and cancer
- Luminescence and Fluorescent Materials
- Cancer-related Molecular Pathways
- Heat shock proteins research
- Microfluidic and Capillary Electrophoresis Applications
- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Microbial Metabolic Engineering and Bioproduction
- Signaling Pathways in Disease
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Autophagy in Disease and Therapy
- Renal Transplantation Outcomes and Treatments
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Analytical Chemistry and Sensors
- Ion channel regulation and function
Northwestern University
1991-2024
Ewha Womans University
2002-2023
Ewha Womans University Medical Center
2020
Korea Institute of Science and Technology
2010-2019
Korea University of Science and Technology
2011-2018
Bio-Medical Science (South Korea)
2018
Hyoja Geriatric Hospital
2018
Seoul National University of Science and Technology
2012-2015
Seoul National University
2015
Kyungpook National University
2005-2012
A simple and low cost chemosensor is described. This sensor could simultaneously detect three biologically important metal ions through fluorogenic (Zn2+) chromogenic (Fe2+, Cu2+, Zn2+) methods in aqueous solution. The function as a "turn-on" fluorescence receptor only to Zn2+ ions. In addition, the be successfully applied detection of intracellular Zn2+. Meanwhile, displayed an obvious red color upon selective binding with Fe2+. Therefore, serve useful tool for discrimination Fe2+ from Fe3+...
The basic helix-loop-helix (bHLH)–Per-Arnt-Sim (PAS) domain transcription factor BMAL1 is an essential component of the mammalian circadian pacemaker. Bmal1 –/– mice lose rhythmicity but also display tendon calcification and decreased activity, body weight, longevity. To investigate whether these diverse functions are tissue-specific, we produced transgenic that constitutively express in brain or muscle examined effects rescued gene expression mice. Circadian rhythms wheel-running activity...
Many transcription coactivators interact with nuclear receptors in a ligand- and C-terminal transactivation function (AF2)-dependent manner. These include activating signal cointegrator 2 (ASC-2), recently isolated transcriptional coactivator molecule, which is amplified human cancers stimulates by numerous other factors. In this report, we show that ASC-2 belongs to steady-state complex of approximately MDa (ASC-2 [ASCOM]) HeLa nuclei. ASCOM contains retinoblastoma-binding protein RBQ-3,...
The spliceosome, a dynamic assembly of proteins and RNAs, catalyzes the excision intron sequences from nascent mRNAs. Recent work has suggested that activity composition spliceosome are regulated by ubiquitination, but underlying mechanisms have not been elucidated. Here, we report spliceosomal Prp19 complex modifies Prp3, component U4 snRNP, with nonproteolytic K63-linked ubiquitin chains. chains increase affinity Prp3 for U5 snRNP Prp8, thereby allowing stabilization U4/U6.U5 snRNP. is...
Knowledge of the cellular targets ROS (reactive oxygen species) and their regulation is an essential prerequisite for understanding ROS-mediated signalling. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) known as a major target protein in oxidative stresses becomes thiolated its active site. However, molecular functional changes oxidized GAPDH, inactive form, have not yet been characterized. To examine modifications under stress, we separated oxidation products by two-dimensional gel...
A new metal ion sensor that contains quinoline and pyridylaminophenol has been synthesized. In acetonitrile in the presence of Zn2+, fluoresces. contrast, aqueous solutions fluoresces Cd2+. The fluorescence molecule is selective for Zn2+ or Cd2+ shows no with other ions. crystal structure complex coordination through amide phenol oxygens, as well amine nitrogen sensor. also acts a colorimetric Co2+ by changing color from colorless to yellow. change not observed unique combination pyridine...
Diabetic foot ulcers represent one of the major and rising health issues, as number diabetic patients is increasing. MicroRNAs (miRNAs) are among various bioactive molecules under investigation for wound healing. The prolonged pro-inflammatory phase in wounds partly attributes to its non-healing nature. Therefore, we hypothesized that miRNA-497, known regulation inflammatory responses, would enhance We screened miRNA candidates, including miRNA-497 wounded skin streptozotocin-induced type 1...
The receptor <bold>1</bold> provides a novel approach for the simultaneous colorimetric recognition of two metal ions Fe<sup>2+</sup> and Cu<sup>2+</sup>.
Neurotensin (NT) is distributed throughout the brain and gastrointestinal tract. Although relationship between NT matrix metalloproteinase-9 (MMP-9) activity in gastric cancer has not been reported, elevation of MMP-9 reported breast, lung, prostate, cancer. The aim our study to investigate underlying signaling mechanism cell lines. Commercial ELISA kits were used for estimation expression, fluorescence resonance energy transfer (FRET) assay was measurement activity. Cell migration invasion...
SMURF2 is a member of the HECT family E3 ubiquitin ligases that have important roles as negative regulator transforming growth factor-β (TGF-β) signaling through ubiquitin-mediated degradation TGF-β receptor I. However, regulatory mechanism largely unknown. In this study, we identified micro(mi)R-195 and miR-497 putatively target using several prediction databases. Both miR-195 bind to 3'-UTR mRNA inhibit expression. Furthermore, regulate SMURF2-dependent TβRI ubiquitination cause activation...
The formation of hyperphosphorylated intracellular Tau tangles in the brain is a hallmark Alzheimer's disease (AD). hyperphosphorylation destabilizes microtubules, promoting neurodegeneration AD patients. To identify suppressors tau-mediated AD, we perform screen using microRNA (miR) library Drosophila and miR-9 family as human tau overexpression phenotypes. CG11070, miR-9a target gene, its mammalian orthologue UBE4B, an E3/E4 ubiquitin ligase, alleviate eye neurodegeneration, synaptic...
Human Fas-associated factor 1 (hFAF1) is a novel protein having multiubiquitin-related domains. We investigated the cellular functions of hFAF1 and found that valosin-containing (VCP), multiubiquitin chain-targeting in degradation ubiquitin-proteasome pathway, binding partner hFAF1. associated with ubiquitinated proteins via newly identified N-terminal UBA domain VCP C-terminal UBX domain. The overexpression truncated inhibited increased cell death. These results suggest to involved pathway....
The transforming growth factor‐β (TGF‐β) signaling pathway is associated with carcinogenesis and various biological processes. SMAD2 SMAD4 , which are putative tumor suppressors, have an important role in TGF‐β signaling. aberrant expression of these genes implicated some cancers. However, the mechanisms dysregulation poorly understood. In this study, we observed that miR‐27a was upregulated lung cancer cell lines patients. addition, were identified as targets by several target prediction...