A5021088098- 3D Printing in Biomedical Research
- Nanofabrication and Lithography Techniques
- Advanced Materials and Mechanics
- Microfluidic and Capillary Electrophoresis Applications
- Surface Modification and Superhydrophobicity
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Adhesion, Friction, and Surface Interactions
- Force Microscopy Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Advanced Sensor and Energy Harvesting Materials
- Electrospun Nanofibers in Biomedical Applications
- Electrowetting and Microfluidic Technologies
- Cancer-related Molecular Pathways
- Innovative Microfluidic and Catalytic Techniques Innovation
- Tissue Engineering and Regenerative Medicine
- Advanced Glycation End Products research
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Glioma Diagnosis and Treatment
- Fluid Dynamics and Thin Films
- Structural Analysis and Optimization
- Metabolism, Diabetes, and Cancer
- Cell Adhesion Molecules Research
Korea Advanced Institute of Science and Technology
2016-2025
Korea Institute of Brain Science
2014-2025
Kootenay Association for Science & Technology
2023
Hanyang University
2020
Seoul National University
2004-2016
Yonsei University
2016
Severance Hospital
2016
Princeton University
2010-2012
Korea Institute of Science and Technology
2006-2012
Korea University
2006
A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the difference between normal epithelial cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 perpendicular, parallel lines) were constructed on bottom polydimethylsiloxane (PDMS) channels in fashion UV-assisted capillary moulding technique. The (MCF10A) (MCF7) each channel was independently measured based detachment...
Abstract This Feature Article aims to provide an in‐depth overview of the recently developed molding technologies termed capillary force lithography (CFL) that can be used control cellular microenvironment towards cell and tissue engineering. Patterned polymer films a fertile ground for controlling various aspects such as cell–substrate cell–cell interactions at micro‐ nanoscale. Patterning thin by typically involves several physical forces capillary, hydrostatic, dispersion forces. If these...
Glioblastoma multiforme (GBM) is the most common brain tumor with very aggressive and infiltrative. Extracellular matrix (ECM) plays pivotal roles in infiltrative characteristics of GBM. To understand invasive characteristic GBM, it necessary to study cell-ECM interaction physiologically relevant biomimetic model that recapitulates GBM-specific ECM microenvironment. Here, we propose microenvironment for studying mode dynamics glioblastoma cell invasion. Using tissue decellularization...
A simple, yet innovative, method is presented for fabricating high-aspect-ratio polymer nanohairs (aspect ratio >20) on a solid substrate by sequential application of molding and drawing thin film. The film was prepared spin coating rigid or flexible substrate, the temperature raised above polymer's glass transition while in conformal contact with poly(urethane acrylate) mold having nanocavities. Consequently, capillary forces induced deformation melt into void spaces filled nanostructure...
We introduce well-defined nanopillar arrays of a poly(ethylene glycol) (PEG) hydrogel as cell culture platform to guide 3D construct primary rat cardiomyocytes in vitro for potential tissue engineering applications. Ultraviolet (UV)-assisted capillary lithography was used fabricate highly uniform approximately 150 nm PEG pillars with 400 height. It found that adhesion significantly enhanced on nanopillars (132 +/- 29 cells/mm2) compared the bare control (39 17 (p < 0.05) but substantially...
We demonstrate a recycled ion-flux through heterogeneous nanoporous junctions, which induce stable ion concentration polarization with an electric field. The junctions are based on integration of ionic hydrogels whose surfaces negatively or positively charged for cationic anionic selectivity, respectively. Such can be matched up in way to achieve continuous operation gradient conductance. Furthermore, the combined used accumulate ions specific region device.
Abstract The high mortality in glioblastoma multiforme (GBM) patients is primarily caused by extensive infiltration into adjacent tissue and subsequent rapid recurrence. There are no clear therapeutic strategies that target the infiltrative subpopulation of GBM mass. Using mesenchymal mode invasion, known to widely infiltrate interacting with various unique components within brain microenvironment such as hyaluronic acid (HA)-rich matrix white matter tracts. However, it unclear how these...
Brain metastasis in breast cancer is particularly deadly, but effective treatments remain out of reach due to insufficient information about the mechanisms underlying brain and potential vulnerabilities brain-metastatic cells. Here, human cells their derivatives (BrMs) were used investigate synthetic lethal interactions BrMs. First, it was demonstrated that c-MYC activity increased BrMs required for ability a mouse xenograft model. Specifically, enhanced by facilitating following processes...
Deprivation of tumor bioenergetics by inhibition multiple energy pathways has been suggested as an effective therapeutic approach for various human tumors. However, this idea not evaluated in glioblastoma (GBM). We hypothesized that dual glycolysis and oxidative phosphorylation could effectively suppress GBM tumorspheres (TS). Effects 2-deoxyglucose (2DG) metformin, alone combination, on GBM-TS were evaluated. Viability, cellular metabolism status, stemness, invasive properties,...
Abstract Gastric cancer (GC) is a common aggressive malignant tumor with high incidence and mortality worldwide. GC classified into intestinal diffuse types according to the histo-morphological features. Because of distinctly different clinico-pathological features, new therapy strategies in vitro preclinical models for two pathological variants necessary. Since extracellular matrix (ECM) influence biological behavior cells, we hypothesized that might be more similarly modeled 3D rather than...
We present a simple and widely applicable method to fabricate micro- nanochannels comprised entirely of crosslinked polyethylene glycol (PEG) by using UV-assisted irreversible sealing bond partially PEG surfaces. The developed here can be used form channels as small ∼50 nm in diameter without sophisticated experimental setup. manufactured channel is homogeneous conduit made completely from non-biofouling PEG, exhibits robust with minimal swelling additional surface modification chemistries,...
Mesenchymal stemlike cells (MSLCs) have been detected in many types of cancer including brain tumors and received attention as stromal the tumor microenvironment. However, cellular mechanisms underlying their participation progression remain largely unexplored. The aim this study was to determine whether MSLCs a tumorigenic role tumors.To figure out molecular glioma invasion, we cultured with co-culture system.Here, show that human glioblastoma (GBM) secrete complement component C5a, which...
Abstract Background Understanding the proteomic-level heterogeneity of tumor microenvironment (TME) in colorectal cancer (CRC) is crucial due to its well-known heterogeneity. While heterogenous CRC has been extensively characterized at molecular subtype level, research into functional fibroblasts, particularly their relationship with extracellular matrix (ECM) alterations, remains limited. Addressing this gap essential for a comprehensive understanding progression and development targeted...
We study the dynamic fracture of thin layers suspensions non-Brownian rigid particles. The impact a projectile triggers liquid-to-solid transition and hole opens in layer. show that occurrence spatial features cracks depend mostly on thickness layer particle volume fraction. In contrast, properties fractured material seem independent Finally, we measure velocity crack tip, from which estimate an effective value shear modulus material.
Highly aligned metal nanowire arrays with feature sizes approaching 10 nm are fabricated. This is made possible by the self-assembly of block copolymers (BCPs) on graphene-wrinkle arrays. Thickness-modulated BCP films confined wrinkled reduced graphene oxide (rGO) surface promote strict alignment self-assembled lamellae in direction film thickness gradient. As a service to our authors and readers, this journal provides supporting information supplied authors. Such materials peer reviewed may...
Abstract Background Human mesenchymal stem cells (hMSCs) are, due to their pluripotency, useful sources of for cell therapy and tissue regeneration. The phenotypes hMSCs are strongly influenced by microenvironment, in particular the extracellular matrix (ECM), composition structure which important regulating fate. In reciprocal manner, properties ECM remodeled hMSCs, but mechanism involved remodeling under topographical stimulus is unclear. this study, we therefore examined effect...
// Junjeong Choi 1, 2, * , Ji-Hyun Lee 3, Ilkyoo Koh 4, Jin-Kyoung Shim 3 Junseong Park Jeong Yong Jeon 5 Mijin Yun Se Hoon Kim 6 Jong In Yook 7 Eui Hyun Hee Chang Sun Ho Min Huh 8 Su Jae 9 Michael Pollak 10 Pilnam 4 Seok-Gu Kang Jae-Ho Cheong 11 1 Department of Pharmacy, College Yonsei Institute Pharmaceutical Sciences, University, Incheon, Republic Korea 2 Brain 21 Plus Project for Medical Science, Seoul, Departments Neurosurgery, Tumor Center, Severance Hospital, University Medicine, Bio...
Ionic hydrogel-based ion concentration polarization devices have been demonstrated as platforms to study nanoscale transport and develop engineering applications, such protein preconcentration ionic diodes/transistors. Using a microfluidic system composed of perm-selective hydrogel, we micro/nanofluidic device for the biological samples using new class mechanism called “capillarity polarization” (CICP). Instead an external electrical voltage source, capillary force hydrogel spontaneously...
We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary moulding. The patterned PEG surfaces have shown 97 +/- 0.5% reduction in adsorption two dimensional 95 1.2% channels comparison to control. Atomic force microscopy measurements indicated that the deposition vesicles...