A5062007761- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Electrospun Nanofibers in Biomedical Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Tissue Engineering and Regenerative Medicine
- Spectroscopy Techniques in Biomedical and Chemical Research
- CAR-T cell therapy research
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Cardiac and Coronary Surgery Techniques
- Nanofabrication and Lithography Techniques
- Cancer Genomics and Diagnostics
- Digital Holography and Microscopy
- Mesenchymal stem cell research
- Additive Manufacturing and 3D Printing Technologies
- Graphene and Nanomaterials Applications
Korea Advanced Institute of Science and Technology
2016-2025
Kootenay Association for Science & Technology
2019-2024
Government of the Republic of Korea
2019-2021
Tohoku University
2020
Massachusetts Institute of Technology
2010-2015
Significance The cancer biology seed-and-soil paradigm recognizes the existence of organ-specific patterns metastasization that drive spread selected primary tumors toward specific secondary loci. However, despite efforts to model organotypic microenvironment, organ specificity metastases needs be elucidated. relevance this study lies in generation a human vascularized which can used investigate and tune extravasation process metastatic tumor cells. Furthermore, beyond mimicking pro- or...
Journal Article Mechanisms of tumor cell extravasation in an vitro microvascular network platform Get access Michelle B. Chen, Chen Department Mechanical Engineering, Massachusetts Institute Technology, Cambridge, MA, USA Search for other works by this author on: Oxford Academic Google Scholar Jordan A. Whisler, Whisler Jessie S. Jeon, Jeon Roger D. Kamm USADepartment Biological E-mail: rdkamm@mit.edu Integrative Biology, Volume 5, Issue 10, October 2013, Pages 1262–1271,...
Tumor cells that disseminate from the primary tumor and survive vascular system can eventually extravasate across endothelium to metastasize at a secondary site. In this study, we developed microfluidic mimic cell extravasation where cancer transmigrate an endothelial monolayer into hydrogel models extracellular space. The experimental protocol is optimized ensure formation of intact prior introduction also observe by having suitable seeding density. Extravasation observed for 38.8% in...
Journal Article Generation of 3D functional microvascular networks with human mesenchymal stem cells in microfluidic systems Get access Jessie S. Jeon, Jeon Department Mechanical Engineering, Massachusetts Institute Technology, 77 Avenue, Cambridge, MA, USA 02139 Search for other works by this author on: Oxford Academic Google Scholar Simone Bersini, Bersini Information, Electronics and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 ItalyCell Tissue Engineering...
Sprouting angiogenesis requires a coordinated guidance from variety of angiogenic factors. Here, we have developed unique hydrogel incorporating microfluidic platform which mimics the physiological microenvironment in 3D under precisely orchestrated gradient soluble factors, VEGF and ANG-1. The system enables quantified investigation chemotactic response endothelial cells during collective sprouting process. While presence alone was sufficient inducing greater number tip cells, addition...
Anti-angiogenic therapy, which suppresses tumor growth by disrupting oxygen and nutrient supply from blood to the tumor, is now widely accepted as a treatment for cancer. To investigate mechanisms of action these anti-angiogenesis drugs, new three dimensional (3D) cell culture-based drug screening models are increasingly employed. However, there no in vitro high-throughput (HTS) angiogenesis assay that can provide uniform culture conditions quantitative assessment physiological responses...
Spatiotemporal analysis of the inflammatory response has been limited by difficulties in vivo imaging and reconstitution inflammation vitro. Here, we present a novel method for establishing vivo-like models microfluidic device quantitatively measuring three-dimensional transmigration neutrophils during process. This enabled us to concurrently characterize transendothelial migration behaviors under influence various stimuli.
Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these...
Tumor microenvironment recapitulation focusing on perfusable multicellular tumor spheroid model construction via anastomosis of endothelial cells through the increases overall growth and penetration anti-cancer drug.
We propose a microfluidic cell-based drug screening platform to investigate combinatory antibiotic effects by automatically generating concentration combinations.
Cancer metastasis, which is prevalent in malignant tumors, present a variety of cases depending on the primary tumor and metastatic site. The cancer metastasis affected by various factors that surround constitute microenvironment. One several factors, oxygen tension, can affect cells induce changes many ways, including motility, directionality, viability. In particular, tension gradient formed within cluster lower toward center from perivascular area. simple efficient designing...
The cytotoxic response of natural killer (NK) cells in a microreactor to surface acoustic waves (SAWs) is investigated, where the SAWs produce an streaming flow. Rayleigh-type form by interdigital transducer propagated along piezoelectric substrate order allow dynamic stimulation functional immune noncontact and rotor-free manner. developed acoustofluidic enables cell culture be set up miniaturized system while maintaining performance agitating media. present SAW creates flow cylindrical...
The construction of well-controllable in vitro models physiological and pathological vascular endothelium remains a fundamental challenge tissue engineering drug development. Here, we present an approach for forming synthetic endothelial extracellular matrix (ECM) that closely resembles the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only region adjacent to cell (EC) monolayer. Culturing EC monolayer on this ECM remarkably enhanced its...