A5031339661- Atherosclerosis and Cardiovascular Diseases
- Coronary Interventions and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- Phagocytosis and Immune Regulation
- Cardiac Imaging and Diagnostics
- Immune cells in cancer
- Optical Coherence Tomography Applications
- Aortic aneurysm repair treatments
- Microfluidic and Bio-sensing Technologies
- Nutrition and Health in Aging
- Systemic Lupus Erythematosus Research
- Peripheral Artery Disease Management
- 3D Printing in Biomedical Research
- Adipokines, Inflammation, and Metabolic Diseases
- Electron Spin Resonance Studies
- Advanced Biosensing Techniques and Applications
- Protease and Inhibitor Mechanisms
- Advanced Glycation End Products research
- Cell Image Analysis Techniques
- Extracellular vesicles in disease
- Diabetic Foot Ulcer Assessment and Management
- Microfluidic and Capillary Electrophoresis Applications
Korea Advanced Institute of Science and Technology
2020-2025
Hanyang University
2019
Anyang University
2019
Dongguk University Ilsan Hospital
2017
Inflammation plays a pivotal role in the pathogenesis of acute coronary syndrome. Detecting plaques with high inflammatory activity and specifically treating those lesions can be crucial to prevent life-threatening cardiovascular events.
In multispectral fluorescence lifetime imaging (FLIm), achieving consistent quality across all spectral channels is crucial for accurately identifying a wide range of fluorophores. However, these essential measurements are frequently compromised by saturation artifacts due to the inherently limited dynamic detection systems. To address this issue, we present SatCompFLImNet, deep learning-based network specifically designed correct in FLIm, facilitating high applications. Leveraging...
With the growing interest in biochips, numerous efforts have been made to recapitulate a more reliable and physiologically relevant environment within chips, resulting significant advances biochip technologies. Although there substantial improvements fabrication technologies, less effort has devoted improving imaging or microscopic methods specialized for observing in-chip structures. As structures become increasingly sophisticated complexity of scaffolds mixed with cells extracellular...
Autofluorescence lifetime (AFL) imaging, a robust technique that enables label-free molecular investigation of biological tissues, is being introduced into the field cardiovascular diagnostics. However, detailed AFL characteristics coronary arteries remain elusive and there lack methodology enabling such characterization.We developed multispectral fluorescence imaging microscopy (FLIM) based on analog-mean-delay. Freshly sectioned atheromas, harvested from 5 swine models, were imaged using...
Photoactivation targeting macrophages has emerged as a therapeutic strategy for atherosclerosis, but limited targetable ability of photosensitizers to the lesions hinders its applications. Moreover, molecular mechanistic insight phototherapeutic effects on atheroma is still lacking. Herein, we developed macrophage near-infrared fluorescence (NIRF) emitting phototheranostic agent by conjugating dextran sulfate (DS) chlorin e6 (Ce6) and estimated feasibility in murine atheroma. Also,...
Abstract Micro-optical coherence tomography (µOCT) is a novel imaging approach enabling visualization of the microstructures biological tissues at cellular or sub-cellular level. However, it has been challenging to develop miniaturized flexible endoscopic µOCT probe allowing helical luminal scanning. In this study, we built with an outer diameter 1.2 mm, which acquires three-dimensional images arterial via scanning axial and lateral resolutions 1.83 µm 3.38 in air, respectively. Furthermore,...
Coronary plaque destabilization involves alterations in microstructure and biochemical composition; however, no imaging approach allows such comprehensive characterization. Herein, the authors demonstrated a simultaneous microstructural assessment of high-risk plaques coronary arteries beating heart using fully integrated optical coherence tomography fluorescence lifetime (FLIm). It was found that components as lipids, macrophages, lipids+macrophages, fibrotic tissues had unique signatures...
Introduction: Fluorescence lifetime imaging (FLIm) is an emerging technique that can visualize detailed molecular properties of atherosclerosis. We conducted in vivo serial characterization human coronary plaques using a fully-integrated, dual-modal, intravascular optical coherence tomography-FLIm (OCT-FLIm) system to explore the natural history biochemical plaque composition. Methods: prospectively enrolled patients with significant artery disease those who underwent 6-month OCT-FLIm...
Multimodal optical coherence tomography (OCT) techniques are promising diagnostic tools to accurately assess high-risk atherosclerotic plaques. For rapid translation into clinical practice, the should be performed through an intravascular imaging catheter without exogenous contrast agents under same procedures as conventional imaging. In this study, we developed a label-free, multispectral, and catheter-based system simultaneously visualize morphological compositional information of coronary...
In this study, we developed a high resolution fluorescence lifetime imaging microscopy to assess atherosclerotic plaque. Various tissue components can be classified using multispectral lifetimes and intensity ratio based on histological study.
Introduction: Autophagy plays a protective role in atherosclerosis and can promote efferocytosis of apoptotic cells. Photoactivation is promising tool for treating cardiovascular disease, induces autophagy macrophages-derived foam cells, however, regulation by photoactivation atherosclerotic plaque remains unknown. Objective: The present study aims to investigate whether macrophage targeted light activation enhances inflamed plaques Methods Results: Targeted photoactivatable agent showed...
Introduction: Fluorescence lifetime imaging (FLIm) allows label-free biochemical characterization of atheroma, however, multispectral FLIm produces massive data throughput making image interpretation problematic. We investigated whether machine learning algorithm can be applied to intravascular OCT-FLIm for automated coronary plaques. Methods and Results: built a fully-integrated, high-speed system low-profile, dual-modal catheter that provide high-resolution OCT images correctly...
We developed a label-free fluorescence lifetime imaging microscopy (FLIM) to quantify the metabolic state of tumor spheroids. obtained 3D FLIM images from different focal plane cancer (MCF7) spheroids for 4 spectral bands which was segregated by custom-made resolving unit. To obtain optically sectioned images, we used an optical fiber instead spatial filter. Statistical analyses showed that signals acquired FLIM—fluorescence intensity and lifetime—reflected well cells constituting These...
Introduction and Hypothesis: Endothelial shear stress (ESS) is the tangential force produced by luminal blood flow on arterial endothelium. Both high ESS low are known to have atherogenic effects, however, it remains poorly understood how these different forces influence coronary atherosclerosis. We evaluated impact of changes biochemical phenotypic difference atheroma, as assessed a novel dual-modal optical coherence tomography-fluorescence lifetime imaging (OCT-FLIm) in vivo beating human...
Photoactivation is a promising theranostic tool to image and stabilize the atherosclerotic plaque by apoptosis induction in macrophages or other vascular cells; however, lack of effective drugs mechanistic understanding hinder its clinical application for cardiovascular disease. Here, we developed macrophage targeted photosensitizer delivery strategy demonstrated that imaging assisted light activation reduced inflammation burden plaques. Mechanistically, photoactivation induced autophagy...
Intravascular optical coherence tomography-fluorescence lifetime imaging (OCT-FLIm) provides co-registered structural and biochemical information of atherosclerotic plaques in a label-free manner. For intuitive image interpretation OCT-FLIm, herein, we present machine learning classifier where key components (lipids, lipids+macrophages, macrophages, fibrotic, normal) related to plaque destabilization are characterized based on the combination multispectral FLIm parameters convolutional OCT...