Hydrogels that mimic biological extracellular matrix (ECM) can provide cells with mechanical support and signaling cues to regulate their behavior. However, despite the ability of hydrogels generate artificial ECM modulate cellular behavior, they often lack strength needed for many tissue constructs. Here, we present reinforced CNT–gelatin methacrylate (GelMA) hybrid as a biocompatible, cell-responsive hydrogel platform creating cell-laden three-dimensional (3D) The addition carbon nanotubes...

Magnetic nanoparticles have gained significant attention as a therapeutic agent for cancer treatment. Herein, we developed chitosan oligosaccharide-stabilized ferrimagnetic iron oxide nanocubes (Chito-FIONs) an effective heat nanomediator hyperthermia. Dynamic light scattering and transmission electron microscopic analyses revealed that Chito-FIONs were composed of multiple 30-nm-sized FIONs encapsulated by polymer shell. Multiple in interior increased the total magnetic moments, which leads...

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. Thus far, however, GelMA mostly obtained from mammalian sources, which are associated with risk of transmission diseases, such as mad cow disease, well certain religious restrictions. In this study, we synthesized using fish-derived gelatin by conventional synthesis method, and evaluated its physical properties cell responses. The lower melting point fish compared to porcine allowed...

Microvesicles (MVs) released by cells are involved in a multitude of physiological events as important mediators intercellular communication. MVs derived from mesenchymal stem (MSCs) contain various paracrine factors the that primarily contribute to their therapeutic efficacy observed numerous clinical trials. As nano-sized and bi-lipid layered vesicles retaining potency equivalent MSCs, MSC-derived have been focus ideal medicinal candidates for regenerative medicine, preferred over MSC...

We present a microfabricated hybrid biopolymer microcantilever, in which the contractile force of self-organized cardiomyocytes can be measured and studied, as prototype for development cell-driven actuators. The microcantilever is made flexible, transparent, biocompatible poly(dimethylsiloxane) substrate, using simple microfabrication technique. Seeding culturing on specific cantilever allows us to perform highly sensitive, quantitative, noninvasive measurement cells real time. motions...

We introduce well-defined nanopillar arrays of a poly(ethylene glycol) (PEG) hydrogel as cell culture platform to guide 3D construct primary rat cardiomyocytes in vitro for potential tissue engineering applications. Ultraviolet (UV)-assisted capillary lithography was used fabricate highly uniform approximately 150 nm PEG pillars with 400 height. It found that adhesion significantly enhanced on nanopillars (132 +/- 29 cells/mm2) compared the bare control (39 17 (p < 0.05) but substantially...

A portable and cost-effective real-time cardiotoxicity biosensor was developed using a CMOS imaging module extracted from commercially available webcam. The detection system consists of module, white LED pinhole. Real-time image processing conducted by comparing reference live frame images. To evaluate the engineered system, effects two different drugs, isoprenaline doxorubicin, on beating rate beat-to-beat variations ESC-derived cardiomyocytes were measured. used to conclude that...

Biologically active materials from marine sources have been receiving increasing attention as they are free the transmissible diseases and religious restrictions associated with use of mammalian resources. Among various other biomaterials sources, alginate fish gelatin (f-gelatin), their inherent bioactivity physicochemical tunability, studied extensively applied in biomedical fields such regenerative medicine, tissue engineering, pharmaceutical products. In this study, by using f-gelatin's...

The gelatin extracted from mammals of porcine and bovine has been prominently used in pharmaceutical, medical, cosmetic products. However, there have some concerns for their usage due to religious, social cultural objections, animal-to-human infectious disease. Recently, marine by-products received growing attention as an alternative mammalian gelatin. In this study, we demonstrate the formation nanogels (NGs) using fish methacryloyl (GelMA) application possibility drug delivery system....

Bioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability the bioink depends on physical properties such as viscosity. However, high viscosity puts shear stress cells and low cannot maintain structure firmly after printing. In this work, we applied dual crosslinkable using Kappa-carrageenan (κ-CA) to overcome existing shortcomings. κ-CA has biocompatibility, biodegradability, shear-thinning ionic...

Digital-light-processing (DLP) three-dimensional (3D) bioprinting, which has a rapid printing speed and high precision, requires optimized biomaterial ink to ensure photocrosslinking for successful printing. However, optimization studies on DLP bioprinting have yet sufficiently explore the measurement of light exposure energy absorbance controls improve printability. In this study, we synchronized wavelength projection base printer with absorption ink. paper, provide stepwise explanation...

Abstract A major clinical hurdle to translate MSC-derived extracellular vesicles (EVs) is the lack of a method scale-up production EVs with customized therapeutic properties. In this study, we tested whether EV by scalable 3D-bioprocessing feasible and improves neuroplasticity in animal models stroke using MRI study. MSCs were cultured 3D-spheroid micro-patterned well. The isolated filter tangential flow filtration characterized electron microscopy, nanoparticle tracking analysis, small RNA...

Abstract Statins are potent inhibitors of cholesterol synthesis. Several statins available with different molecular and pharmacokinetic properties. Simvastatin is more lipophilic than pravastatin has a higher affinity to phospholipid membranes atorvastatin, allowing its passive diffusion through the cell membrane. In vitro studies on bone marrow stromal cells, osteoblast-like embryonic stem cells have shown cholesterol-independent anabolic effects metabolism; alas, were supplemented in...

Abstract Recent advances in stem cell research have demonstrated the importance of microenvironmental cues directing fate towards specific lineages. For instance, size embryoid body (EB) was shown to play a role differentiation. Other studies used adhesive RGD peptides direct endothelial cells. In this study, materials and cell‐based approaches are combined by using microwell arrays produce size‐controlled EBs encapsulating resulting aggregates high molecular weight PEG‐4 arm acrylate with...

Bioink is the main component of 3D bioprinting process and crucial for generation sophisticated structures through precise spatial control. Therefore, bioink's core material must have characteristics that support good printability as well biocompatibility. However, there a lack bioinks developed satisfy these at same time. In this work, our aim was to develop bioink satisfies needs both biocompatibility effectively utilizing hydrocolloid materials. To do so, carboxymethyl cellulose (CMC)...

Stem-cell-derived extracellular vesicles (EVs) are emerging as an alternative approach to stem cell therapy. Successful lyophilization of EVs could enable convenient storage and distribution EV medicinal products at room temperature for long periods, thus considerably increasing the accessibility therapeutics patients. In this study, we aimed identify appropriate lyoprotectant composition reconstitution stem-cell-derived EVs. MSC-derived were lyophilized using different lyoprotectants, such...

Embryonic stem cell (ESC) culture is fragmented and laborious involves operator decisions. Most protocols consist of 3 individual steps: maintenance, embryoid body (EB) formation, differentiation. Integration will assist automation, ultimately aiding scale-up to clinically relevant numbers. These problems were addressed by encapsulating undifferentiated murine ESCs (mESCs) in 1.1% (w/v) low-viscosity alginic acid, 0.1% (v/v) porcine gelatin hydrogel beads (d = 2.3 mm). Six hundred containing...