A5039331709- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Bone Tissue Engineering Materials
- Nanofabrication and Lithography Techniques
- Cancer Cells and Metastasis
- Force Microscopy Techniques and Applications
- Additive Manufacturing and 3D Printing Technologies
- Piezoelectric Actuators and Control
- Muscle Physiology and Disorders
- Advanced Surface Polishing Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Spaceflight effects on biology
- Extracellular vesicles in disease
- Congenital heart defects research
- Advanced Sensor and Energy Harvesting Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biomedical and Engineering Education
- Micro and Nano Robotics
- Cardiomyopathy and Myosin Studies
Johns Hopkins University
2010-2025
University of Washington
2015-2024
Johns Hopkins Medicine
2012-2024
NanoBio (United States)
2024
Korea University
2006-2024
California Institute for Regenerative Medicine
2022-2024
Dongguk University
2021
Institute for Stem Cell Biology and Regenerative Medicine
2014-2018
Hawaii Agriculture Research Center
2018
Cardiovascular Research Center
2014-2017
The ability to print and pattern all the components that make up a tissue (cells matrix materials) in three dimensions generate structures similar tissues is an exciting prospect of bioprinting. However, majority materials used so far for bioprinting cannot represent complexity natural extracellular (ECM) thus are unable reconstitute intrinsic cellular morphologies functions. Here, we develop method cell-laden constructs with novel decellularized (dECM) bioink capable providing optimized...
Heart tissue possesses complex structural organization on multiple scales, from macro- to nano-, but nanoscale control of cardiac function has not been extensively analyzed. Inspired by ultrastructural analysis the native tissue, we constructed a scalable, nanotopographically controlled model myocardium mimicking in vivo ventricular organization. Guided mechanical cues provided underlying hydrogel, constructs displayed anisotropic action potential propagation and contractility characteristic...
Mechanical forces play important roles in the regulation of various biological processes at molecular and cellular level, such as gene expression, adhesion, migration, cell fate, which are essential to maintenance tissue homeostasis. In this review, we discuss emerging bioengineered tools enabled by microscale technologies for studying mechanical biology. addition traditional mechanobiology experimental techniques, review recent advances microelectromechanical systems (MEMS)-based approaches...
Abstract Directed migration by contact guidance is a poorly understood yet vital phenomenon, particularly for carcinoma cell invasion on aligned collagen fibres. We demonstrate that single cells, architectures providing cues induce constrained focal adhesion maturation and associated F-actin alignment, consequently orchestrating anisotropic traction stresses drive orientation directional migration. Consistent with this understanding, relaxing spatial constraints to either through reduction...
Understanding the phenotypic development of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is a prerequisite to advancing regenerative cardiac therapy, disease modeling, and drug screening applications. Lack consistent hiPSC-CM in vitro data can be largely attributed inability conventional culture methods mimic structural, biochemical, mechanical aspects myocardial niche accurately. Here, we present nanogrid array comprised nanogrooved topographies, with groove widths...
Microrobots that can be precisely guided to target lesions have been studied for in vivo medical applications. However, existing microrobots challenges such as biocompatibility, biodegradability, actuation module, and intra- postoperative imaging. This study reports visualized with real-time x-ray magnetic resonance imaging (MRI) magnetically tumor feeding vessels transcatheter liver chemoembolization vivo. The microrobots, composed of a hydrogel-enveloped porous structure nanoparticles,...
The development of a wireless undulatory tadpole robot using ionic polymer–metal composite (IPMC) actuators is presented. In order to improve the thrust robot, biomimetic motion fin tail implemented. overall size underwater microrobot prototype, shaped as tadpole, 96 mm in length, 24 width, and 25 thickness. It has one polymer driven by cast IPMC actuator, an internal (wireless) power source, embedded controller. controlled changing frequency duty ratio input voltage. Experimental results...
A simple method was developed to fabricate polyethylene glycol (PEG) nanostructures using capillary lithography mediated by ultraviolet (UV) exposure. Acrylate-containing PEG monomers, such as dimethacrylate (PEG-DMA, MW = 330), were photo-cross-linked under UV exposure generate patterned structures. In comparison unpatterned films, hydrophobicity of nanostructure modified surfaces significantly enhanced. This could be attributed trapped air in the supported water contact angle measurements....
Abstract This work reports the design of and experimentation with a topographically patterned cell culture substrate variable local density anisotropy as facile efficient platform to guide organization migration cells in spatially desirable patterns. Using UV‐assisted capillary force lithography, an optically transparent microstructured layer UV curable poly(urethane acrylate) resin is fabricated employed cell‐culture after coating fibronectin. With pattern present single substrate,...
Nanoscale topography of artificial substrates can greatly influence the fate stem cells including adhesion, proliferation, and differentiation. Thus design manipulation nanoscale cell culture platforms or scaffolds are great importance as a strategy in tissue engineering applications. In this report, we propose that graphene oxide (GO) film is an efficient platform for modulating structure function human adipose‐derived (hASCs). Using self‐assembly method, successfully coated GO on glass...
This work reports the design of and experimentation with a topographically patterned cell culture substrate variable local density anisotropy as facile efficient platform to guide organization migration cells in spatially desirable patterns. Using UV-assisted capillary force lithography, an optically transparent microstructured layer UV curable poly(urethane acrylate) resin is fabricated employed cell-culture after coating fibronectin. With pattern present single substrate, differential...
It is becoming increasingly evident that cell biology research can be considerably advanced through the use of bioengineered tools enabled by nanoscale technologies. Recent advances in nanopatterning techniques pave way for engineering biomaterial surfaces control cellular interactions from nano- to microscale, allowing more precise quantitative experimentation capturing multi-scale aspects complex tissue physiology vitro. The spatially and temporally controlled display extracellular...
Neurons in the central nervous system (CNS) fail to regenerate axons after injuries due diminished intrinsic axon growth capacity of mature neurons and hostile extrinsic environment composed a milieu inhibitory factors. Recent studies revealed that targeting particular group extracellular factors is insufficient trigger long-distance regeneration. Instead antagonizing growing list impediments, tackling common target mediates inhibition offers an alternative strategy promote Neuronal cone,...