A5071480295- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Additive Manufacturing and 3D Printing Technologies
- Neuroscience and Neural Engineering
- Advanced Sensor and Energy Harvesting Materials
- Anesthesia and Pain Management
- Conducting polymers and applications
- Silk-based biomaterials and applications
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Nanoparticle-Based Drug Delivery
- Pluripotent Stem Cells Research
- Hemostasis and retained surgical items
- Pain Mechanisms and Treatments
- Intraocular Surgery and Lenses
- Fuel Cells and Related Materials
- Analytical Chemistry and Sensors
- Surgical Sutures and Adhesives
- Cell Adhesion Molecules Research
- Prosthetics and Rehabilitation Robotics
- Cardiovascular and Diving-Related Complications
- Caveolin-1 and cellular processes
Johns Hopkins University
2020-2024
University of Washington
2013-2022
California Institute for Regenerative Medicine
2022
Boston Children's Hospital
2011-2018
Harvard University
2011-2018
Seattle University
2018
The Royal Free Hospital
2015
University College London
2015
Massachusetts Institute of Technology
2012-2013
Foundation for the National Institutes of Health
2013
Bacterial sepsis is a serious clinical condition that can lead to multiple organ dysfunction and death despite timely treatment with antibiotics fluid resuscitation. We have developed an approach clearing bacteria endotoxin from the bloodstream, using magnetic nanoparticles (MNPs) modified bis-Zn-DPA, synthetic ligand binds both Gram-positive Gram-negative bacteria. Magnetic microfluidic devices were used remove MNPs bound Escherichia coli, bacterium commonly implicated in bacterial sepsis,...
Understanding the phenotypic development of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is a prerequisite to advancing regenerative cardiac therapy, disease modeling, and drug screening applications. Lack consistent hiPSC-CM in vitro data can be largely attributed inability conventional culture methods mimic structural, biochemical, mechanical aspects myocardial niche accurately. Here, we present nanogrid array comprised nanogrooved topographies, with groove widths...
We report a nanoparticulate system capable of targeting the heart after myocardial infarction (MI). Targeting is based on overexpression angiotensin II type 1 (AT1) receptor in infarcted heart. Liposomes 142 nm diameter were conjugated with ligand specific to AT1. The nanoparticles able specifically target cardiac cells vitro, and intravenous injection vivo. This may be useful for delivering therapeutic agents
We report on the development of bioinspired cardiac scaffolds made from electroconductive acid-modified silk fibroin-poly(pyrrole) (AMSF+PPy) substrates patterned with nanoscale ridges and grooves reminiscent native myocardial extracellular matrix (ECM) topography to enhance structural functional properties cultured human pluripotent stem cells (hPSC)-derived cardiomyocytes. Nanopattern fidelity was maintained throughout fabrication functionalization processes, no loss in conductive behavior...
With current plans for manned missions to Mars and beyond, the need better understand, prevent, counteract harmful effects of long-duration spaceflight on body is becoming increasingly important. In this study, an automated heart-on-a-chip platform was flown International Space Station a 1-mo mission during which contractile cardiac function monitored in real-time. Upon return Earth, engineered human heart tissues (EHTs) were further analyzed with ultrastructural imaging RNA sequencing...
Cell-biomaterial interactions can be controlled by modifying the surface chemistry or nanotopography of material, to induce cell proliferation and differentiation if desired. Here we combine both approaches in forming silk nanofibers (SNFs) containing gold nanoparticles (AuNPs) subsequently chemically fibers. Silk fibroin mixed with seed was electrospun form SNFs doped (SNFseed). Following reduction, there a 2-fold increase particle diameter confirmed appearance strong absorption peak at 525...
Electrically conductive materials provide a suitable platform for the in vitro study of excitable cells, such as skeletal muscle due to their inherent conductivity and electroactivity. Here it is demonstrated that bioinspired electroconductive nanopatterned substrates enhance myogenic differentiation maturation. The topographical cues from highly aligned collagen bundles form extracellular matrix tissue are mimicked using nanopatterns created with capillary force lithography. Electron beam...
Biosourced and biodegradable polymers for additive manufacturing could enable the rapid fabrication of parts a broad spectrum applications ranging from healthcare to aerospace. However, limited number these materials are suitable vat photopolymerization processes. Herein, we report two-step process fabricate robust protein-based constructs using commercially available laser-based stereolithography printer. Methacrylated bovine serum albumin (MA-BSA) was synthesized formulated into aqueous...
Current tissue engineering methods lack the ability to properly recreate scaffold-free, cell-dense tissues with physiological structures. Recent studies have shown that use of nanoscale cues allows for precise control over large-area 2D structures without restricting cell growth or density. In this study, we developed a simple and versatile platform combining thermoresponsive nanofabricated substratum (TNFS) incorporating nanotopographical gel casting method fabrication scaffold-free 3D...
Aberrant neuronal activity in injured peripheral nerves is believed to be an important factor the development of neuropathic pain. Pharmacological blockade that has been shown mitigate onset associated molecular events nervous system. However, results preventing pain behaviors by providing prolonged nerve have mixed. Furthermore, experimental techniques used date provide were limited clinical potential they would require surgical implantation. To address these issues, we liposomes (SDLs)...
Endothelial physiology is regulated not only by humoral factors, but also mechanical factors such as fluid shear stress and the underlying cellular matrix microenvironment. The purpose of present study was to examine effects topographical cues on endothelial secretion cytokines/chemokines in vitro. Human cells were cultured nanopatterned polymeric substrates with different ratios ridge groove widths (1:1, 1:2, 1:5) stiffnesses (6.7 MPa 2.5 GPa) presence absence 1.0 ng/mL TNF-α. levels...
Vascular smooth muscle cells (vSMCs) retain the ability to undergo modulation in their phenotypic continuum, ranging from a mature contractile state proliferative, secretory state. vSMC differentiation is modulated by complex array of microenvironmental cues, which include biochemical milieu and architecture stiffness extracellular matrix. In this study, we demonstrate that using UV-assisted capillary force lithography (CFL) engineer polyurethane substratum defined nanotopography stiffness,...
Hypertrophic cardiomyopathy (HCM) affects approximately 600,000 people in the United States. Loss-of-function mutations Myosin Binding Protein C3, MYBPC3, are most common genetic cause of HCM, with majority resulting haploinsufficiency. To restore cardiac we use an adeno-associated virus (AAV9) vector and engineer optimized expression cassette a minimal promoter cis-regulatory elements (TN-201) to enhance packaging efficiency cardiomyocyte expression. Rather than simply preventing...
Intravenous regional anesthesia (IVRA; Bier block) is commonly used to anesthetize an extremity for surgery. Limitations of the procedure include pain from required tourniquet, toxicity that can occur systemic release local anesthetics, and lack postoperative relief. We hypothesized nanoencapsulation anesthetic would prolong enhance safety. Here, we developed ∼15 nm micellar bupivacaine formulation (M-Bup) tested it in a rat tail vein IVRA model, which active agents were restricted by...
<h3>Background and Objectives</h3> Quaternary lidocaine derivatives (QLDs) have recently received much attention because of their potential application in prolonged or sensory-selective local anesthesia. However, associated tissue toxicity is an impeding factor that makes QLDs unfavorable for clinical use. Based on the proposed intracellular site action, we hypothesized nerve blocks obtained from lower concentrations would be enhanced by coapplication extracellularly acting 1 sodium-channel...
Despite possessing substantial regenerative capacity, skeletal muscle can suffer from loss of function due to catastrophic traumatic injury or degenerative disease. In such cases, engineered tissue grafts hold the potential restore and improve patient quality life. Requirements for successful integration with host musculature include cell alignment that mimics architecture directional functionality, as well vascularization ensure survival. Here, we have developed biomimetic nanopatterned...
Ocular local anesthetics currently used in routine clinical practice for corneal anesthesia are short acting and their ability to delay healing makes them unsuitable long-term use. In this study, we examined the effect of site 1 sodium channel blocker tetrodotoxin (TTX) on duration anesthesia, applied with either proparacaine (PPC) or chemical permeation enhancer octyl-trimethyl ammonium bromide (OTAB). The test solutions was also studied.Solutions TTX, PPC, OTAB, singly combination, were...
Engineering mature and functional skeletal muscle tissue requires an ability to promote the structural organization differentiation of cultured myoblasts. On page 137, D.-H. Kim, S.G. Im, co-workers describe a bio-inspired electroconductive culture platform, wherein nanopatterned substrate mimicking extracellular matrix native is covered with thin layer gold. Engineered tissues using this platform feature hypertrophic uniaxially aligned myotubes increased expression levels myogenic markers.