A5013362793- Prostate Cancer Treatment and Research
- Cellular Mechanics and Interactions
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- Cancer Cells and Metastasis
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cancer-related gene regulation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced Chemical Sensor Technologies
- Cancer Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Molecular Biology Techniques and Applications
- Bone and Dental Protein Studies
- Cell Adhesion Molecules Research
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Cardiovascular Health and Disease Prevention
- Cancer Immunotherapy and Biomarkers
Rutgers, The State University of New Jersey
2020-2025
Johnson University
2020-2025
Johns Hopkins University
2012-2024
Imaging Center
2012-2024
Physical Sciences (United States)
2012-2024
Johns Hopkins Medicine
2012-2024
Thomas Jefferson University
2015-2022
Rutgers Sexual and Reproductive Health and Rights
2021
Sidney Kimmel Comprehensive Cancer Center
2013-2019
Jichi Medical University
2019
Our incomplete understanding of osteoarthritis (OA) pathogenesis has significantly hindered the development disease-modifying therapy. The functional relationship between subchondral bone (SB) and articular cartilage (AC) is unclear. Here, we found that changes SB architecture altered distribution mechanical stress on AC. Importantly, latter well aligned with pattern transforming growth factor beta (TGFβ) activity in AC, which essential regulation AC homeostasis. Specifically, TGFβ...
// Takumi Shiraishi 1 , James E. Verdone Jessie Huang 5 Ulf D. Kahlert 6 R. Hernandez Gonzalo Torga Jelani C. Zarif Tamir Epstein 7 Robert Gatenby Annemarie McCartney 8 Jennifer H. Elisseeff Steven M. Mooney S. An 4,5,9 and Kenneth J. Pienta 1,2,3,4 Department of Urology, The Buchanan Brady Urological Institute, Johns Hopkins University, Baltimore, MD, USA 2 Oncology, 3 Pharmacology Molecular Sciences, 4 Chemical Biomolecular Engineering, Environmental Health Bloomberg School Public Health,...
The ion transporter NKCC1 determines brain tumor cell migration by regulating the interplay between adhesion and growth factor signaling, is a potential therapeutic target to treat cancer.
TWIST1 is a transcription factor critical for development that can promote prostate cancer metastasis. During embryonic development, and HOXA9 are coexpressed in mouse then silenced postnatally. Here we report coexpression reactivated human primary tumors further enriched metastases, correlating with survival. formed complex WDR5 the lncRNA Hottip/HOTTIP, members of MLL/COMPASS-like H3K4 methylases, which regulate chromatin Hox/HOX cluster during development. overexpression led to...
Abstract Pathways that control, or can be exploited to alter, the increase in airway smooth muscle (ASM) mass and cellular remodeling occur asthma are not well defined. Here we report expression of odorant receptors (ORs) belonging superfamily G-protein coupled (GPCRs), as canonical olfaction machinery (G olf AC3) human bronchi. In primary cultures isolated ASM, identified mRNA for multiple ORs. Strikingly, OR51E2 was most highly enriched OR transcript mapped olfactome lung-resident cells. a...
Abstract Cardiomyocytes undergo significant structural and functional changes after birth, these fundamental processes are essential for the heart to pump blood growing body. However, due challenges of isolating single postnatal/adult myocytes, how individual newborn cardiomyocytes acquire multiple aspects mature phenotype remains poorly understood. Here we implement large-particle sorting analyze myocytes from neonatal adult hearts. Early exhibit wide-ranging transcriptomic size...
Airway smooth muscle (ASM) cells in culture stiffen when exposed to contractile agonists. Such cell stiffening may reflect activation of the apparatus as well polymerization cytoskeletal biopolymers. Here we have assessed relative contribution these mechanisms cultured ASM stimulated with serotonin (5-hydroxytryptamine; 5-HT) presence or absence drugs that inhibit either myosin-based contraction filamentous (F) actin. Magnetic twisting cytometry was used measure stiffness, and associated...
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONConclusionReferencesCITING ARTICLES
Previous studies have linked a reduction in pH airway, caused by either environmental factors, microaspiration of gastric acid or inflammation, with airway smooth muscle (ASM) contraction and increased resistance. Neural mechanisms been shown to mediate response reductions < 6.5; whether reduced extracellular (pHo) has direct effects on ASM is unknown.Intracellular signalling events stimulated pHo human cultured cells were examined immunoblotting, phosphoinositide hydrolysis calcium...
Transforming growth factor β1 (TGF-β1), a cytokine whose levels are elevated in the airways of patients with asthma, perpetuates airway inflammation and modulates structural cell remodeling. However, role TGF-β1 excessive narrowing or hyperresponsiveness (AHR), remains unclear. In this study, we set out to investigate direct effects on human smooth muscle (HASM) shortening hyperresponsiveness. The dynamics AHR single-cell excitation-contraction coupling were measured precision-cut lung...
Recently, bitter taste receptors (TAS2Rs) were found in the lung and act to relax airway smooth muscle (ASM) via intracellular Ca(2+) concentration signaling generated from restricted phospholipase C activation. As potential therapy, TAS2R agonists could be add-on treatment when patients fail achieve adequate bronchodilation with chronic β-agonists. The β(2)-adrenergic receptor (β(2)AR) of ASM undergoes extensive functional desensitization. It remains unknown whether this desensitization...
Bitter taste receptors (TAS2Rs) have recently been found to be expressed on human airway smooth muscle (HASM), and their activation results in marked relaxation. These agents proposed as a new class of bronchodilators the treatment obstructive lung diseases because they act via different mechanism than β-agonists. The TAS2R signal transduction pathway HASM has multiple elements that are potentially subject regulation by inflammatory, genetic, epigenetic mechanisms associated with asthma. To...
The structural elements of the vascular wall, namely, extracellular matrix and smooth muscle cells (SMCs), contribute to overall stiffness vessel. In this study, we examined crosslinking-dependent crosslinking-independent roles tissue transglutaminase (TG2) in function stiffness.SMCs were isolated from aortae TG2-/- wild-type (WT) mice. Cell adhesion was by using electrical cell-substrate impedance sensing PicoGreen assay. motility a Boyden chamber proliferation EdU incorporation assays....
Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity and mortality. Cigarette smoke (CS) drives development progression. The epithelial barrier damaged by CS with increased monolayer permeability. However, the molecular changes that this disruption interaction between adhesion proteins cytoskeleton are not well defined. We hypothesized alters integrity increasing cell contractility decreasing in epithelia. Normal human airway cells primary COPD were exposed to air or...
Inhaled β-agonists are effective at reversing bronchoconstriction in asthma, but the mechanism by which they exert this effect is unclear and controversial. PKA historically accepted effector, although assumption made on basis of associative not direct evidence. Recent studies have asserted that exchange protein activated cAMP (Epac), PKA, mediates relaxation airway smooth muscle (ASM) observed with β-agonist treatment. This study aims to clarify role prorelaxant effects ASM. Inhibition...
Childhood-onset schizophrenia (COS) is a rare and severe form of the disorder, with more striking abnormalities respect to prepsychotic developmental disorders abnormities in brain development compared later-onset schizophrenia. We previously documented that COS patients, their healthy siblings adult-onset patients (AOS), carry significantly chromosomal copy number variations, spanning large genomic regions (>100 kb) (Ahn et al. 2014). Here, we interrogated contribution common polygenic...
Bitter taste receptors (TAS2Rs) are expressed on human airway smooth muscle (HASM) and evoke marked relaxation. Agonist interaction with TAS2Rs activates phospholipase C increases compartmentalized intracellular Ca2+ ([Ca2+]i) via inositol 1,4,5 triphosphate. In cells, the G protein gustducin couples TAS2R to C; however, we find very low levels of Gαgust mRNA or in HASM. We hypothesized that another HASM transmits function. signaling [Ca2+]i, extracellular signal-regulated kinase (ERK) 1/2,...