A5029063787- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Bladder and Urothelial Cancer Treatments
- PARP inhibition in cancer therapy
- Advanced X-ray Imaging Techniques
- Immunotherapy and Immune Responses
- Image Processing Techniques and Applications
- AI in cancer detection
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Effects of Radiation Exposure
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced Proteomics Techniques and Applications
- Mechanisms of cancer metastasis
- Fibroblast Growth Factor Research
- Colorectal Cancer Surgical Treatments
University of Maryland, Baltimore
2023
Drexel University
2021
Johns Hopkins University
2014-2020
University of Baltimore
2014-2020
University of Pennsylvania
2019
Johns Hopkins Medicine
2014-2018
Recent genomic studies challenge the conventional model that each metastasis must arise from a single tumor cell and instead reveal metastases can be composed of multiple genetically distinct clones. These intriguing observations raise question: How do polyclonal emerge primary tumor? In this study, we used multicolor lineage tracing to demonstrate seeding by clusters is frequent mechanism in common mouse breast cancer, accounting for >90% metastases. We directly observed multicolored across...
// Takumi Shiraishi 1 , James E. Verdone Jessie Huang 5 Ulf D. Kahlert 6 R. Hernandez Gonzalo Torga Jelani C. Zarif Tamir Epstein 7 Robert Gatenby Annemarie McCartney 8 Jennifer H. Elisseeff Steven M. Mooney S. An 4,5,9 and Kenneth J. Pienta 1,2,3,4 Department of Urology, The Buchanan Brady Urological Institute, Johns Hopkins University, Baltimore, MD, USA 2 Oncology, 3 Pharmacology Molecular Sciences, 4 Chemical Biomolecular Engineering, Environmental Health Bloomberg School Public Health,...
There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells (PBMCs) into the M1 or M2 macrophage cell types. Each type is then verified using flow cytometric analysis of cell-surface markers. Human have potential and macrophages, both which demonstrate varying degrees antigen overlap. Using multiple surface markers with current polarization protocols, our data reveal limitations used methods, such as highly...
Mutant KRAS drives glycolytic flux in lung cancer, potentially impacting aberrant protein glycosylation. Recent evidence suggests of glucose into the hexosamine biosynthetic pathway (HBP). HBP is required for various glycosylation processes, such as N- or O-glycosylation and glycolipid synthesis. However, its function during tumorigenesis poorly understood. One contributor proposed target KRAS-driven cancers a developmentally conserved epithelial plasticity program called...
Experimental and clinical evidence suggests that N-myc downregulated gene 1 (NDRG1) functions as a suppressor of prostate cancer metastasis. Elucidating pathways drive survival invasiveness NDRG1-deficient cells can help in designing therapeutics to target metastatic cells. However, the molecular mechanisms lead increased remain largely unknown. In this study, we demonstrate tumors have decreased integrin expression reduced cell adhesion motility. Our data indicate loss NDRG1 differentially...
Abstract Quantitative analysis of morphological changes in a cell nucleus is important for the understanding nuclear architecture and its relationship with pathological conditions such as cancer. However, dimensionality imaging data, together great variability shapes, presents challenges 3D analysis. Thus, there compelling need robust morphometric techniques to carry out population-wide We propose new approach that combines modeling, analysis, interpretation characteristics nuclei nucleoli...
Physicians trained in opioid use disorder (OUD) harm reduction can mitigate overdose deaths by prescribing naloxone and educating patients about its use. Unfortunately, many physicians possess OUD stigma. Training during medical school presents an opportunity to reduce stigma improve reversal knowledge. This study assessed the efficacy of Opioid Overdose Awareness Reversal (OOART) evaluated equivalency online in-person OOART. Methods: Voluntary training was delivered first-year (M1) students...
An epithelial to mesenchymal transition (EMT) has been shown be a necessary precursor prostate cancer metastasis. Additionally, the differential expression and splicing of mRNAs identified as key means distinguish from cells by qPCR, western blotting immunohistochemistry. However, few markers exist differentiate between these flow cytometry. We previously developed two cell lines, PC3-Epi (epithelial) PC3-EMT (mesenchymal). RNAseq was used determine membrane proteins on PC3-Epi/EMT....
Cell deformation is regulated by complex underlying biological mechanisms associated with spatial and temporal morphological changes in the nucleus that are related to cell differentiation, development, proliferation, disease. Thus, quantitative analysis of size shape nuclear structures 3D microscopic images important not only for investigating organization, but also detecting treating pathological conditions such as cancer. While many efforts have been made develop characteristics 2D or...
BACKGROUND Prostate cancer progression is concomitant with quantifiable nuclear structure and texture changes as compared to non-cancer tissue. Malignant associated an epithelial–mesenchymal transition (EMT) program whereby epithelial cells take on a mesenchymal phenotype dissociate from tumor mass, invade, disseminate distant metastatic sites. The objective of this study was determine if prostate have different morphology. METHODS Murine tibia injections PC3 (PC3-Epi) (PC3-EMT) were...
Zinc finger E-box binding homeobox 1 (ZEB1) is a transcription factor that plays central role in the epithelial to mesenchymal transition (EMT) of cancer cell lines. Studies on its regulation have mostly focused negative 3'UTR miR200c. Interestingly, it has been previously reported androgen receptor (AR) regulates ZEB1 expression breast and prostate cancers. In order validate this, various promoter deletions were cloned into luciferase reporter system elucidate contribution two putative...
Abstract Quantitative analysis of morphological changes in a cell nucleus is important for the understanding nuclear architecture and its relationship with pathological conditions such as cancer. However, dimensionality imaging data, together great variability shapes, presents challenges 3D analysis. Thus, there compelling need robust morphometric techniques to carry out population-wide We propose new approach that combines modeling, analysis, interpretation characteristics nuclei nucleoli...
Abstract Cell deformation is regulated by complex underlying biological mechanisms associated with spatial and temporal morphological changes in the nucleus that are related to cell differentiation, development, proliferation, disease. Thus, quantitative analysis of size shape nuclear structures 3D microscopic images important not only for investigating organization, but also detecting treating pathological conditions such as cancer. While many efforts have been made develop characteristics...
Bone metastasis is a lethal and incurable disease. It the result of dissemination cancer cells to bone marrow. Due difficulty in sampling detection, few techniques exist efficiently consistently detect quantify disseminated tumor (DTCs) marrow patients. Because mouse models represent crucial tool with which study metastasis, we developed novel method for simple selection-free detection quantification DTCs mice. We have used this protocol human murine xenograft, syngeneic, genetically...
Abstract Our previous work has demonstrated that exposure of M2 - Tumor Associated Macrophages M2-TAMs) to epithelial cancer cells can induce an Epithelial Mesenchymal Transition (EMT). The resulting mesenchymal exhibit a more aggressive phenotype, which places patients at higher risk relapse and death. Although most are cured by radical prostatectomy or radiation, it been the majority have disseminated tumor in their bone marrow time primary treatment approximately 10-15% these men will...
Abstract Background: Epithelial-mesenchymal transition (EMT) is associated with metastasis in human prostate cancer (PCa). As metastatic tumors are the major cause of death most patients, identifying instances EMT patients may serve as a good prognostic indicator progressive disease. Abnormal morphologic deformation cell nucleus strongly an aggressive phenotype and computer-aided quantitative nuclear morphometry (QNM) has been used to quantify this deformation. In present study, we go...