A5074588897- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Hedgehog Signaling Pathway Studies
- Cell Image Analysis Techniques
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Axon Guidance and Neuronal Signaling
- Cancer Immunotherapy and Biomarkers
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Neuroblastoma Research and Treatments
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Brain Metastases and Treatment
- Cancer Research and Treatments
- Circadian rhythm and melatonin
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
Mayo Clinic in Florida
2017-2024
WinnMed
2017-2024
Jacksonville College
2017-2024
Neurological Surgery
2020-2023
Nemours Children's Clinic
2020
Universidad de Navarra
2010-2019
Johns Hopkins Medicine
2012-2019
Johns Hopkins University
2012-2019
Sidney Kimmel Comprehensive Cancer Center
2016-2017
University of Baltimore
2015-2016
The ion transporter NKCC1 determines brain tumor cell migration by regulating the interplay between adhesion and growth factor signaling, is a potential therapeutic target to treat cancer.
We show that pluripotent human cells can be differentiated within an elastomer chip into a microenvironment mimicking the brain parenchyma.
PTEN is a PIP3 phosphatase that antagonizes oncogenic PI3-kinase signalling. Due to its critical role in suppressing the potent signalling pathway, it one of most mutated tumour suppressors, especially brain tumours. It generally thought deficiencies predominantly result from either loss expression or enzymatic activity. By analysing malignant glioblastoma primary cells derived 16 our patients, we report mutations block localization at plasma membrane and nucleus without affecting lipid...
Abstract A high throughput histology (microTMA) platform was applied for testing drugs against tumors in a novel 3D heterotypic glioblastoma brain sphere (gBS) model consisting of tumor cells, iPSC-derived neurons, glial cells and astrocytes grown spheroid. The differential responses gBS normal neuronal to sustained treatments with anti-cancer temozolomide (TMZ) doxorubicin (DOX) were investigated. exposed TMZ or DOX over 7-day period. Untreated increased size 4-week culture period, however,...
Glioblastoma multiforme (GBM) is a highly lethal human cancer thought to originate from self-renewing and therapeutically-resistant population of glioblastoma stem cells (GSCs). The intrinsic mechanisms enacted by GSCs during 3D tumor formation, however, remain unclear, especially in the stages prior angiogenic/immunological infiltration. In this study, we performed deep characterization genetic, immune, metabolic profiles GBM organoids several patient-derived (GBMO). Despite being devoid...
Glioblastoma multiforme is a heterogeneous and infiltrative cancer with dismal prognosis. Studying the migratory behavior of tumor-derived cell populations can be informative, but it places high premium on precision in vitro methods relevance vivo conditions. In particular, analysis 2D migration may not reflect invasion into 3D extracellular matrices vivo. Here, we describe method that allows time-resolved studies primary single-cell resolution fibrillar surface closely mimics migration. We...
Glioblastoma (GBM) is the most aggressive primary brain tumor in adults. The mechanisms that confer GBM cells their invasive behavior are poorly understood. electroneutral Na+-K+-2Cl- co-transporter 1 (NKCC1) an important cell volume regulator participates migration. We have shown inhibition of NKCC1 leads to decreased migration, vitro and vivo. now report on role cytoskeletal dynamics. show display a significant decrease F-actin content upon knockdown (NKCC1-KD). To determine potential...
Chemical modification of small molecule hydrophobic drugs is a clinically proven strategy to devise prodrugs with enhanced treatment efficacy.While this prodrug improves the parent drug's water solubility and pharmacokinetic profile, it typically compromises potency against cancer cells due retarded drug release rate reduced cellular uptake efficiency.Here we report on supramolecular design self-assembling (SAPD) much improved while maintaining high cells.We found that camptothecin (CPT)...
Abstract Galvanotaxis is a complex process that represents the collective outcome of various contributing mechanisms, including asymmetric ion influxes, preferential activation voltage-gated channels, and electrophoretic redistribution membrane components. While large number studies have focused on up- downstream signaling pathways, little known about how surrounding microenvironment may interact contribute to directional response. Using customized galvanotaxis chip capable carrying out...
Glioblastoma (GBMs) is the most common and aggressive type of primary brain tumor in adults with dismal prognosis despite radical surgical resection coupled chemo- radiotherapy. Recent studies have proposed use small-molecule inhibitors, including verteporfin (VP), to target oncogenic networks cancers. Here we report efficient encapsulation water-insoluble VP poly(lactic-co-glycolic acid) microparticles (PLGA MP) ∼1.5 μm diameter that allows tunable, sustained release. Treatment naked...
Abstract Background Glioblastomas (GBMs) are the main primary brain tumors in adults with almost 100% recurrence rate. Patients lateral ventricle proximal GBMs (LV-GBMs) exhibit worse survival compared to distal locations for unknown reasons. One hypothesis is proximity of these cerebrospinal fluid (CSF) and its chemical cues that can regulate cellular phenotype. We therefore investigated role CSF on GBM gene expression a CSF-induced gene, SERPINA3, malignancy vitro vivo. Methods utilized...
Abstract Cancer is a leading cause of death and disease worldwide. However, while the survival for patients with primary cancers improving, ability to prevent metastatic cancer has not. Once develop metastases, their prognosis dismal. A critical step in metastasis transit cells circulatory system. In this hostile microenvironment, variations pressure flow can change cellular behavior. effects that circulation on process remain unclear. To further understand process, we engineered closed-loop...
Nanomedicine can improve traditional therapies by enhancing the controlled release of drugs at targeted tissues in body. However, there still exists disease- and therapy-specific barriers that limit efficacy such treatments. A major challenge developing effective for one most aggressive brain tumors, glioblastoma (GBM), is affecting cancer cells while avoiding damage to surrounding healthy parenchyma. Here, we developed poly(ethylene glycol) (PEG)-poly(beta-amino ester) (PBAE)...
Glioblastoma (GBM) is the most common primary brain cancer in adults where tumor cell heterogeneity and sex differences influence clinical outcomes. Here, we functionally characterize three male female patient-derived GBM lines, identify protumorigenic BTICs, create novel preclinical models of GBM. Cell lines were evaluated on following features: proliferation, stemness, migration, tumorigenesis, characteristics, sensitivity to radiation, TMZ, rh
It is well known that sonic hedgehog signaling pathway plays a vital role during early embryonic development. also responsible for stem cell renewal and development of several cancers like colorectal breast carcinoma major brain tumors as medulloblastoma glioblastoma. The in the neuroblastoma has not been thoroughly investigated. In this study, we attempted to determine expression Bmi-1 marker Shh downstream target genes glioma-associated oncogene homolog 1 (GLI1), protein patched (PTCH1),...
Glioblastoma (GBM) stem cells (GSCs) represent tumor-propagating with stem-like characteristics (stemness) that contribute disproportionately to GBM drug resistance and tumor recurrence. Understanding the mechanisms supporting GSC stemness is important for developing therapeutic strategies targeting GSC-dependent oncogenic mechanisms. Using GBM-derived neurospheres, we identified cell surface tetraspanin family member CD151 as a novel regulator of glioma stemness, self-renewal capacity,...