A5077770404- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neural Engineering
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- Neurological disorders and treatments
- EEG and Brain-Computer Interfaces
- Tissue Engineering and Regenerative Medicine
- Advanced Memory and Neural Computing
- Molecular Communication and Nanonetworks
- Photoreceptor and optogenetics research
- Graphene and Nanomaterials Applications
- Conducting polymers and applications
- Transcranial Magnetic Stimulation Studies
- Viral Infectious Diseases and Gene Expression in Insects
- ATP Synthase and ATPases Research
- Perovskite Materials and Applications
- MXene and MAX Phase Materials
- Cardiac Ischemia and Reperfusion
- Quantum Dots Synthesis And Properties
- Axon Guidance and Neuronal Signaling
- Chromatin Remodeling and Cancer
- biodegradable polymer synthesis and properties
Technical University of Munich
2021-2024
Johns Hopkins Medicine
2011-2022
Johns Hopkins University
2011-2022
Max Planck Institute for Intelligent Systems
2017-2021
Karlsruhe Institute of Technology
2020-2021
FIZ Karlsruhe – Leibniz Institute for Information Infrastructure
2021
University of Baltimore
2019
Stuttgart Observatory
2017
Biodegradable polymeric nanoparticles have the potential to be safer alternatives viruses for gene delivery; however, their use has been limited by poor efficacy in vivo. In this work, we synthesize and characterize delivery evaluate DNA of herpes simplex virus type I thymidine kinase (HSVtk) combined with prodrug ganciclovir (GCV) a malignant glioma model. We investigated polymer structure two rat cell lines, 9L F98, discover nanoparticle formulations more effective than leading commercial...
Wireless powering of magnetoelectric nanoelectrodes is used for deep brain stimulation in freely moving and transgene-free mice.
siRNA nanomedicines can potentially treat many human diseases, but safe and effective delivery remains a challenge. DNA polymers such as poly(β-amino ester)s (PBAEs) generally cannot effectively deliver require chemical modification to enable encapsulation delivery. An optimal nanomaterial needs be able bind self-assemble with molecules that are shorter stiffer than plasmid in order form stable nanoparticles, promote efficient release upon entry the cytoplasm. To address these concerns, we...
Despite our growing molecular-level understanding of glioblastoma (GBM), treatment modalities remain limited. Recent developments in the mechanisms cell fate regulation and nanomedicine provide new avenues by which to treat manage brain tumors via delivery molecular therapeutics. Here, we have developed bioreducible poly(β-amino ester) nanoparticles that demonstrate high intracellular efficacy, low cytotoxicity, escape from endosomes, promotion cytosol-targeted environmentally triggered...
A new 320-member polymer library of end-modified poly(β-amino ester)s was synthesized. This chosen such that small differences to the structures component backbone, side-chain, and end-group monomers could be systematically simultaneously evaluated. The in vitro transfection efficacy cytotoxicity DNA nanoparticles formed from this were assessed. approach not only enabled us synthesize test a large variety rapidly but also provided with robust data set analyze for effect structural...
Tissue engineering using mesenchymal stem cells (MSCs) holds great promise for regenerating critically sized bone defects. While the marrow-derived MSC is most widely studied stromal/stem cell type this application, its rarity within marrow and painful isolation procedure have motivated investigation of alternative sources. Adipose-derived (ASCs) are more abundant easily procured; furthermore, they also possess robust osteogenic potency. these two types considered very similar, there a...
Improved delivery materials are needed to enable siRNA transport across biological barriers, including the blood-brain barrier (BBB), treat diseases like brain cancer. We engineered bioreducible nanoparticles for systemic patient-derived glioblastoma cells in an orthotopic mouse tumor model. first utilized a newly developed biomimetic vitro model evaluate and optimize performance of at crossing microvascular endothelium. performed transmission electron microscopy imaging which indicated that...
Clinical translation of polymer-based nanocarriers for systemic delivery RNA has been limited due to poor colloidal stability in the blood stream and intracellular cytosol. To address these limitations, this study reports a new strategy incorporating photocrosslinking bioreducible nanoparticles improved extracellularly rapid release intracellularly. In design, polymeric contain ester bonds hydrolytic degradation disulfide environmentally triggered small interfering (siRNA) These...
Described here is the synthesis and characterization of a novel, bioreducible linear poly(β-amino ester) designed to condense siRNA into nanoparticles efficiently release it upon entering cytoplasm. Delivery using this polymer achieved near-complete knockdown fluorescent marker gene in primary human glioblastoma cells with no cytotoxicity.
Protein- and peptide-based manufacturing of self-assembled supramolecular functional materials has been a formidable challenge for biomedical applications, being complex in structure immunogenic nature. In this context, self-assembly short amino acid sequences as simplified building blocks to design metal–biomolecule frameworks (MBioFs) is an emerging field research. Here, we report facile, bioinspired route anisotropic nanostructure synthesis using gold binding peptides (10–15mers) secreted...
Abstract The growing field of nanoscale neural stimulators offers a potential alternative to larger scale electrodes for brain stimulation. Nanoelectrodes made magnetoelectric nanoparticles (MENPs) can provide an invasive stimulation via magnetic‐to‐electric signal transduction. However, the effect is complex phenomenon and challenging probe experimentally. Consequently, quantifying voltage provided by MENPs difficult, hindering precise regulation control limiting their practical...
ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication the EditorNEXTDevelopment of a PEG Derivative Containing Hydrolytically Degradable HemiacetalsBranden Reid, Stephany Tzeng, Andrew Warren, Kristen Kozielski, and Jennifer Elisseeff*View Author Information Johns Hopkins University, Smith Bldg. 5031, 400 N. Broadway, Baltimore, Maryland 21231, United States*Corresponding author. E-mail: [email protected]Cite this: Macromolecules 2010, 43, 23, 9588–9590Publication Date...
A non-viral nanoparticle-based transfection method delivers Sox2 or Olig2 into primary human astrocytes to convert the neurons and oligodendrocyte progenitors, respectively, providing a highly translatable approach for engineering repair injured central nervous tissues.
Xanthine oxidase (XO) activity contributes to both abnormal excitation-contraction (EC) coupling and cardiac remodeling in heart failure (HF). beta-Adrenergic hyporesponsiveness abnormalities Ca(2+) cycling proteins are mechanistically linked features of the HF phenotype. Accordingly, we hypothesized that XO influences beta-adrenergic responsiveness expression genes whose products participate deranged EC coupling. We measured inotropic (dP/dt(max)), lusitropic (tau), vascular (elastance;...