A5036009607- Neonatal and fetal brain pathology
- Neonatal Respiratory Health Research
- Neurogenetic and Muscular Disorders Research
- Epilepsy research and treatment
- Olfactory and Sensory Function Studies
- Congenital Heart Disease Studies
- Receptor Mechanisms and Signaling
- Congenital Anomalies and Fetal Surgery
- Neonatal Health and Biochemistry
- Mitochondrial Function and Pathology
- Biochemical Analysis and Sensing Techniques
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Genetic and Kidney Cyst Diseases
- Dialysis and Renal Disease Management
- Adipose Tissue and Metabolism
- Congenital heart defects research
- Immune cells in cancer
- Birth, Development, and Health
- Diet and metabolism studies
- Infant Development and Preterm Care
- Atherosclerosis and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Sodium Intake and Health
- Gut microbiota and health
University of Washington
2014-2025
University of Pittsburgh Medical Center
2024
Seattle Children's Hospital
2016-2023
UCSF Benioff Children's Hospital
2023
University of California, San Francisco
2023
University of Pittsburgh
2022-2023
Child Neurology Associates
2023
Harvard Stem Cell Institute
2016-2020
Harvard University
2016-2020
Seattle University
2017-2018
Short chain fatty acid (SCFA) metabolites are byproducts of gut microbial metabolism that known to affect host physiology via G protein-coupled receptor (GPCRs). We previously showed an acute SCFA bolus decreases blood pressure (BP) in anesthetized mice, effect mediated primarily Gpr41. In this study, our aims were identify the cellular localization Gpr41 and determine its role BP regulation. localized vascular endothelium using RT-PCR: is detected intact vessels (with endothelium) but...
Abstract Pathways that control, or can be exploited to alter, the increase in airway smooth muscle (ASM) mass and cellular remodeling occur asthma are not well defined. Here we report expression of odorant receptors (ORs) belonging superfamily G-protein coupled (GPCRs), as canonical olfaction machinery (G olf AC3) human bronchi. In primary cultures isolated ASM, identified mRNA for multiple ORs. Strikingly, OR51E2 was most highly enriched OR transcript mapped olfactome lung-resident cells. a...
The EMBRACE study (Clinical Trials No. NCT02462759) evaluated nusinersen in infants/children with infantile- or later-onset spinal muscular atrophy (SMA) who were ineligible for the ENDEAR and CHERISH studies.Participants randomized to intrathecal (12-mg scaled equivalent dose; n = 14) sham procedure (n 7) part 1 (~14 months) subsequently received open-label ~24 months 2 of study.Part was stopped early after demonstration motor function benefit ENDEAR. There no nusinersen-related adverse...
There is a large body of evidence that cellular metabolism governs inflammation, and inflammation contributes to the progression atherosclerosis. However, whether mitochondrial DNA synthesis affects macrophage function atherosclerosis pathology not fully understood. Here we show, by transcriptomic analyzes plaque macrophages, spatial single cell transcriptomics atherosclerotic plaques, functional experiments, (mtDNA) in macrophages are triggered vascular adhesion molecule 1 (VCAM-1) under...
Olfactory receptors (ORs) are G protein-coupled that detect odorants in the olfactory epithelium, and comprise largest gene family genome. Identification of OR ligands typically requires surface expression heterologous cells; however, ORs rarely traffic to cell when exogenously expressed. Therefore, most orphan with no known ligands. To date, studies have utilized non-cleavable rhodopsin (Rho) tags and/or chaperones (i.e. Receptor Transporting Protein, RTP1S, Ric8b Gαolf) improve expression....
C1q/TNF-related proteins (CTRPs) are a family of secreted regulators glucose and lipid metabolism. Here, we describe CTRP11, novel phylogenetically conserved member the C1q family. Our studies revealed that white brown adipose major tissues express its expression is acutely regulated by changes in metabolic state. Within tissue, CTRP11 primarily expressed stromal vascular cells. As multimeric protein, forms disulfide-linked oligomers. Although N-terminal Cys-28 Cys-32 dispensable for...
Defining conserved molecular pathways in animal models of successful cardiac regeneration could yield insight into why adult mammals have inadequate after injury. Insight the transcriptomic landscape early from model organisms will shed light on evolutionarily regeneration.Here we describe a cross-species screen 3 for regeneration: axolotl, neonatal mice, and zebrafish. Apical resection to remove ≈10% 20% ventricular mass was carried out these organisms. RNA-sequencing analysis performed...
Abstract Visceral adipose tissue (VAT) is a metabolic organ known to regulate fat mass, and glucose nutrient homeostasis. VAT an active endocrine gland that synthesizes secretes numerous bioactive mediators called ‘adipocytokines/adipokines’ into systemic circulation. These adipocytokines act on organs of importance like the liver skeletal muscle. Multiple preclinical in vitro studies showed strong evidence roles regulation disorders diabetes, obesity insulin resistance. Adipocytokines, such...
Predicting neurodevelopmental outcome for neonates with hypoxic-ischemic encephalopathy (HIE) is important clinical decision-making, care planning, and parent communication. We examined the relationship between EEG background among children enrolled in a trial of erythropoietin or placebo HIE treated therapeutic hypothermia.Participants had recorded throughout hypothermia. was classified as normal, discontinuous, severely abnormal (defined burst suppression, low voltage suppressed, status...
Cerebral palsy (CP) is the most common motor disability in childhood, with a worldwide prevalence of 1.5-4/1,000 live births. Hypoxic-ischemic encephalopathy (HIE) contributes to burden CP, but long-term neuropathological findings this association remain limited.Thirty-four term Macaca nemestrina macaques were included study: 9 control animals delivered by cesarean section and 25 perinatal asphyxia after 15-18 min umbilical cord occlusion (UCO). UCO randomized saline (n = 11), therapeutic...
Short-chain fatty acids (SCFAs) are fermentation by-products of gut microbes which have been linked to positive effects on host physiology; the most abundant SCFA is acetate. Exogenous administration acetate alters metabolism, immune function, and blood pressure, making it a biologic interest. The attributed activation G-protein–coupled receptors other proteins (i.e., HDACs), often occurring at locations distant from such as pancreas or kidney. However, due technical difficulties costs,...
OBJECTIVES In infants with hypoxic-ischemic encephalopathy (HIE), conflicting information on the association between early glucose homeostasis and outcome exists. We characterized glycemic profiles in first 12 hours after birth their death neurodevelopmental impairment (NDI) neonates moderate or severe HIE undergoing therapeutic hypothermia. METHODS This post hoc analysis of High-dose Erythropoietin for Asphyxia Encephalopathy trial included n = 491 who had blood (BG) values recorded within...
Background Nusinersen is the only approved treatment for all spinal muscular atrophy (SMA) subtypes and delivered intrathecally. Distorted anatomy instrumentation preclude standard approaches intrathecal access, necessitating alternative techniques delivery. The purpose of this study to report technical success adverse events transforaminal delivery nusinersen. Methods 28 patients, mean age 24.1±9.8 years (range 10.0–51.0 years), with intermediate or late onset SMA, underwent a combined 200...