A5036941639- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Microtubule and mitosis dynamics
- Glioma Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Cellular transport and secretion
- Signaling Pathways in Disease
- Adenosine and Purinergic Signaling
- Hereditary Neurological Disorders
- Cellular Mechanics and Interactions
- Neuropeptides and Animal Physiology
- Protein Kinase Regulation and GTPase Signaling
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Ion channel regulation and function
- Developmental Biology and Gene Regulation
- 3D Printing in Biomedical Research
- Retinal Development and Disorders
Seoul National University
2018-2025
Korea University of Science and Technology
2014-2018
Bio-Medical Science (South Korea)
2017-2018
Korea Institute of Brain Science
2012-2017
Korea Institute of Science and Technology
2012-2017
Korean Association Of Science and Technology Studies
2012-2017
Convergence Research Center for Diagnosis Treatment and Care System of Dementia
2016-2017
Johns Hopkins Medicine
2010-2016
Johns Hopkins University
2010-2016
Pohang University of Science and Technology
2000-2007
Neurons in the central nervous system (CNS) fail to regenerate axons after injuries due diminished intrinsic axon growth capacity of mature neurons and hostile extrinsic environment composed a milieu inhibitory factors. Recent studies revealed that targeting particular group extracellular factors is insufficient trigger long-distance regeneration. Instead antagonizing growing list impediments, tackling common target mediates inhibition offers an alternative strategy promote Neuronal cone,...
Abstract In native tissues, cellular and acellular components are anisotropically organized often aligned in specific directions, providing structural mechanical properties for actuating biological functions. Thus, engineering alignment not only allows emulation of tissue structures but might also enable implementation functionalities. However, achieving desired is challenging, especially three-dimensional constructs. By exploiting the elastomeric property polydimethylsiloxane...
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial and sporadic Parkinson's disease (PD). Elevated activity is associated with LRRK2 toxicity, but substrates that mediate neurodegeneration remain poorly defined. Given increasing evidence suggesting a role membrane vesicle trafficking, here we systemically screened Rab GTPases, core regulators vesicular dynamics, as potential investigated functional consequence such phosphorylation cells vivo. In vitro...
Mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) is a dual-specificity phosphatase that involved in the regulation of cell survival, differentiation and apoptosis through inactivating MAPKs by dephosphorylation. Here, we provide evidence for role MKP-1 glutamate-induced death HT22 hippocampal cells primary mouse cortical neurons. We suggest that, during oxidative stress, C (PKC) delta becomes activated induces sustained activation extracellular signal-regulated 1/2 (ERK1/2)...
Glycogen synthase kinase 3 (GSK3) is emerging as a key regulator of several aspects neuronal morphogenesis including polarization, axon growth, and branching. Multiple signaling pathways have been identified that control PI3K, Rho-GTPases, Par3/6, TSC-mTOR, PKA-LKB1. However, how these are coordinated not clear. As GSK3 exhibits crosstalk with each it has the potential to integrate polarity signals in polarization. After neurons establish polarity, acts an important mediator regulation...
Epidermal growth factor receptor variant III (EGFRvIII) has been associated with glioma stemness, but the direct molecular mechanism linking two is largely unknown. Here, we show that EGFRvIII induces expression and secretion of pigment epithelium-derived (PEDF) via activation signal transducer activator transcription 3 (STAT3), thereby promoting self-renewal tumor progression stem cells (GSCs). Mechanistically, PEDF sustained GSC by Notch1 cleavage, generated intracellular domain (NICD)...
Katanin was the first microtubule (MT)-severing enzyme discovered, but how katanin executes MT severing remains poorly understood. Here, we report X-ray crystal structures of apo and ATPγS-bound states catalytic AAA domain human p60 at 3.0 2.9 Å resolution, respectively. Comparison two reveals conformational changes induced by ATP binding such ensure hexamer stability. Moreover, uncover structural details pore loops (PLs) show that Arg283, a residue unique to among MT-severing enzymes,...
Abstract A culture system that can recapitulate myelination in vitro will not only help us better understand the mechanism of and demyelination, but also find out possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple reproducible using mouse motor neurons (MNs) Schwann cells (SCs). Dissociated are plated on feeder layer SCs, which interact with wrap around axons MNs as they differentiate culture. In our MN-SC coculture system, survived over 3...
Abstract Leucine-rich repeat kinase 2 ( LRRK2 ), originally identified as a causative genetic factor in Parkinson’s disease, is now associated with number of pathologies. Here, we show that brain injury induces robust expression endogenous and suggest role after injury. We found various vitro vivo models traumatic (TBI) markedly enhanced neurons also increased the level hypoxia-inducible (HIF)-1α. Luciferase reporter assay chromatin immunoprecipitation revealed direct binding HIF-1α proximal...
We investigated the effects of 17beta-estradiol, an estrogen, on [(3)H]norepinephrine ([(3)H]NE) secretion in PC12 cells. Pretreatment with 17beta-estradiol reduced 70 mM K(+)-induced [(3)H]NE a concentration-dependent manner half-maximal inhibitory concentration (IC(50)) 2 +/- 1 microM. The cytosolic free Ca(2+) ([Ca(2+)](i)) rise was also when cells were treated (IC(50) = 15 microM). Studies voltage-sensitive calcium channel (VSCC) antagonists such as nifedipine and omega-conotoxin GVIA...
The epidermal growth factor (EGF) receptor has an important role in cellular proliferation, and the enzymatic activity of phospholipase C (PLC)-γ1 is regarded to be critical for EGF-induced mitogenesis. In this study, we report first time a complex composed PLC-γ1 D2 (PLD2). co-immunoprecipitated with PLD2 COS-7 cells. results vitro binding analysis co-immunoprecipitation cells show that Src homology (SH) 3 domain binds proline-rich motif within Phox (PX) PLD2. interaction between EGF...
Neurotransmitter release is modulated in an activity-dependent manner. We showed previously that repetitive stimulation of nicotinic acetylcholine receptor (nAChR) induced potentiation (ADP) large dense-core vesicle (LDCV) exocytosis chromaffin cells. Here we report protein kinase C (PKC)-ε critically involved ADP. Stimulation nAChR activation PKC-ε, and inhibition PKC-ε by expression the dominant-negative mutant (DN-PKC-ε) or short interfering (siRNA) against abolished ADP via decreasing...
Glycogen synthase kinases 3 (GSK3) α and β are expressed in the nervous system, disruption of GSK3 signaling has been implicated a wide range neurodevelopmental psychiatric disorders. Although several studies have established role much less is known about isoform-specific functions. Here, we examined GSK3α GSK3β developing neocortex by performing utero electroporation with specific small interfering RNAs targeting each isoform. We found that depletion either or commonly promoted...
Abstract Schwann cells (SCs), the primary glia in peripheral nervous system (PNS), display remarkable plasticity that fully mature SCs undergo dedifferentiation and convert to repair upon nerve injury. Dedifferentiated provide essential support for PNS regeneration by producing signals enhance survival axon regrowth of damaged neurons, but identities neurotrophic factors remain incompletely understood. Here we show express secrete progranulin (PGRN), depending on differentiation status SCs....