A5063560544- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- PI3K/AKT/mTOR signaling in cancer
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Galectins and Cancer Biology
- Sphingolipid Metabolism and Signaling
- Growth Hormone and Insulin-like Growth Factors
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- Peroxisome Proliferator-Activated Receptors
- Animal Genetics and Reproduction
- Acute Myeloid Leukemia Research
- S100 Proteins and Annexins
- Muscle Physiology and Disorders
- Hippo pathway signaling and YAP/TAZ
Korea Brain Research Institute
2019-2025
Ulsan National Institute of Science and Technology
2014-2023
Korea Basic Science Institute
2022
Korea University of Science and Technology
2019
Korea Institute of Brain Science
2019
Pohang University of Science and Technology
2005-2014
Seoul National University
1988-1995
Ewha Womans University
1994
Yonsei University
1994
National Heart Lung and Blood Institute
1987-1991
Epidermal growth factor (EGF) rapidly stimulates the formation of inositol 1,4,5-trisphosphate in a variety cell types. Previously we have found that intact cells stimulation phospholipase C (PLC) activity by EGF is correlated with retention increased amounts PLC phosphotyrosine immunoaffinity matrix, suggesting EGF-receptor tyrosine kinase phosphorylates PLC. We now define parameters mechanism which addition to A-431 immunoisolation and demonstrate increases phosphorylation reversibly...
Dysregulation of O-GlcNAc modification catalyzed by transferase (OGT) and O-GlcNAcase (OGA) contributes to the etiology chronic diseases aging, including cancer, cardiovascular disease, type 2 diabetes, Alzheimer's disease. Here we found that natural aging in wild-type mice was marked a decrease OGA OGT protein levels an increase O-GlcNAcylation various tissues. Genetic disruption resulted constitutively elevated embryos led neonatal lethality with developmental delay. Importantly, observed...
Shank1, Shank2, and Shank3 constitute a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank directly interacts with GKAP Homer, thus potentially bridging <i>N</i>-methyl-d-aspartate receptor-PSD-95-GKAP complex mGluR-Homer synapses (Naisbitt, S., Kim, E., Tu, J. C., Xiao, B., Sala, Valtschanoff, J., Weinberg, R. Worley, P. F., Sheng, M. (1999) <i>Neuron</i> 23, 569–582; Naisbitt, Yuan, P., Petralia, Brakeman, Doan, A., Aakalu, V. K., Lanahan,...
2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) is a widespread environmental pollutant with many toxic effects, including endocrine disruption, reproductive dysfunction, immunotoxicity, liver damage, and cancer. These are mediated by TCDD binding to activating the aryl hydrocarbon receptor (AhR), basic helix-loop-helix transcription factor. In this regard, targeting AhR using novel small molecule inhibitors an attractive strategy for development of potential preventive agents. study,...
Murine hybridoma cell lines secreting antibodies against the three bovine isozymes of phosphoinositide-specific phospholipase C (PLC) were established: 6, 23, and 12 obtained for PLC-I (150 kDa), PLC-II (145 PLC-III (85 respectively. The purified from ascites fluid, their properties studied in detail. All cross-reacted with corresponding PLC enzymes, but not other two isozymes, suggesting that enzymes contain very different antigenic determinants. six elicited by also human rat enzyme,...
that cytosolic fractions of bovine brain contain two phosphoinositidespecific phospholipase C (PLC), PLC-I and PLC-11.In this paper purification procedures properties these forms enzyme are presented.The enzymes exhibit similar substrate specificity.Both PLC-I1 catalyze the hydrolysis phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), phosphatidylinositol-4,5-bisphosphate (PIP2).Yet, they respond differently to activators such as Ca2+ nucleotides inhibitory divalent metal...
We previously reported that cytosolic fractions of bovine brain contain two immunologically distinct forms phospholipase C (PLC), PLC-I and PLC-II. now report the purification another form inositolphospholipid-specific from cytosol, designated PLC-III, comparison catalytic properties three isozymes. Approximately 450 micrograms pure PLC-III was obtained 36 brains, it had a final specific activity 30-40 mumol phosphatidylinositol hydrolyzed per min mg enzyme in presence 0.1% deoxycholate....
Antibodies against an inositol phospholipid-specific phospholipase C purified from bovine brain were used to screen rat lambda gt11 expression cDNA libraries. Complete sequences of three inserts yielded a cumulative sequence 5106 base pairs. The deduced protein had 1289 amino acids with calculated molecular weight 148,431. determination open reading frame was aided by the acid 21 tryptic peptides isolated C. Only 9 residues total 140 determined for enzyme different those cDNA. Two regions...
The mammalian target of rapamycin (mTOR) interacts with raptor to form the protein complex mTORC1 (mTOR 1), which plays a central role in regulation cell growth response environmental cues. Given that glucose is primary fuel source and biosynthetic precursor, how signaling coordinated metabolism has been an important question. Here, we found glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binds Rheb inhibits signaling. Under low-glucose conditions, GAPDH prevents from...
Increased O -GlcNAcylation can confer neuroprotective effects against necroptosis in the context of Alzheimer’s disease.
Blocking phosphorylation of peroxisome proliferator-activated receptor (PPAR)γ at Ser(273) is one the key mechanisms for antidiabetes drugs to target PPARγ. Using high-throughput screening, we here describe that Gleevec blocks cyclin-dependent kinase 5-mediated PPARγ devoid classical agonism as a antagonist ligand. In high fat-fed mice, improved insulin sensitivity without causing severe side effects associated with other PPARγ-targeting drugs. Furthermore, reduces lipogenic and...
Phosphoinositide-specific phospholipase C (PLC) plays a pivotal role in the signal transduction of various cellular responses. However, although it is undeniably important that modulators PLC activity be identified, no direct modulator has been identified until now. In this study, by screening more than 10,000 different compounds human neutrophils, we compound strongly enhances superoxide-generating activity, which well known to PLC-dependent. The active...
Abstract Serum amyloid A (SAA) is a major acute-phase reactant, and has been demonstrated to mediate proinflammatory cellular responses. Although SAA used as an indicator for variety of inflammatory diseases, the role in synovial hyperplasia proliferation endothelial cells, pathological hallmark rheumatoid arthritis (RA), yet be elucidated. In this study, we have that promotes human fibroblast-like synoviocytes (FLS). addition, protects RA FLS against apoptotic death induced by serum...
We previously reported that cytosolic fractions of bovine brain contain two immunologically distinct phosphoinositide-specific phospholipases C (PLCs), PLC-I and PLC-II. In this report the subcellular distribution PLC-II in homogenates was measured using RIA. Significant differences were found forms PLC 100,000 X g supernatants (cytosolic fraction) homogenized hypotonic buffer 2 M KCl extracts washed pellets (particulate fraction). More than 90% fractions, whereas PLC-I-like molecules...