A5009761057- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Escherichia coli research studies
- Evolution and Genetic Dynamics
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Neuroinflammation and Neurodegeneration Mechanisms
- Zoonotic diseases and public health
- Yersinia bacterium, plague, ectoparasites research
- Chemical Reactions and Isotopes
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Alzheimer's disease research and treatments
Tufts University
2021-2024
Sungkyunkwan University
2014-2019
Samsung Medical Center
2017-2019
Samsung (South Korea)
2014-2016
Increased O -GlcNAcylation can confer neuroprotective effects against necroptosis in the context of Alzheimer’s disease.
The widespread use of antibiotics has placed bacterial pathogens under intense pressure to evolve new survival mechanisms. Genomic analysis 51,229 Mycobacterium tuberculosis (Mtb)clinical isolates identified an essential transcriptional regulator, Rv1830, herein called resR for resilience as a frequent target positive (adaptive) selection. mutants do not show canonical drug resistance or tolerance but instead shorten the post-antibiotic effect, meaning that they enable Mtb resume growth...
Abstract The increased reliance on colistin for treating multidrug-resistant Gram-negative bacterial infections has resulted in the emergence of colistin-resistant Pseudomonas aeruginosa . We attempted to identify genetic contributors resistance vitro evolved isogenic and -susceptible strains two P. lineages (P5 P155). Their evolutionary paths acquisition loss were also tracked. Comparative genomic analysis revealed 13 five determinants P5 P155 lineages, respectively. Lipid A mutants was...
Persister cells following antibiotic exposure may cause failure of treatment. The synergistic effects combinations with respect to eliminating persister were investigated based on their characteristics.For Acinetobacter baumannii clinical isolates, assays performed using colistin, amikacin, imipenem and ciprofloxacin in various ways, including antibiotics combination sequentially. phenotypes observed through analysis ATP concentration, membrane potential transmission electron microscopy.Each...
Colistin susceptibility in Pseudomonas aeruginosa is associated with a lipopolysaccharide (LPS) structure that controlled by the modulation of several two-component regulatory systems. In this study, we attempted to elucidate role these systems development colistin resistance P. aeruginosa. pmrA-, phoP-, parR- or cprR-inactivated mutants were constructed from colistin-susceptible P5 strain. Colistin-resistant (P5R, P5ΔpmrA-R, P5ΔphoP-R, P5ΔparR-R and P5ΔcprR-R) developed vitro wild-type...
Persister cells are responsible for antibiotic treatment failure and the emergence of resistance. The synergistic lethal effects combinations on persister were investigated using Pseudomonas aeruginosa isolates.Persister assays performed P. clinical isolates colistin, amikacin, ciprofloxacin cefepime, individually in combination. ATP concentrations measured morphological changes observed transmission electron microscopy (TEM). expression relA, spoT obg genes was evaluated persister-cell...
We recently demonstrated a high rate of colistin dependence in Acinetobacter baumannii isolates exposed to vitro. In the present study, we obtained colistin-resistant (H08-391R) and colistin-dependent mutant (H08-391D) from colistin-susceptible parental strain (H08-391). found that converted into stable (H08-391D-R) vitro after four serial passages without colistin. H08-391D H08-391D-R were both harbor defective lipid A, as indicated by matrix-assisted laser desorption ionization-time flight...
Difficulties in antibiotic treatment of Mycobacterium tuberculosis (Mtb) are partly thought to be due heterogeneity growth. Although the ability bacterial pathogens regulate growth is crucial control homeostasis, virulence and drug responses, single-cell cell cycle behaviours Mtb poorly characterized. Here we use time-lapse, imaging coupled with mathematical modelling observe asymmetric size, interdivision time elongation speed. We find that, contrary smegmatis, initiates not only from old...
The ability of bacterial pathogens to regulate growth is crucial control homeostasis, virulence, and drug response. Yet, we do not understand the cell cycle behaviors Mycobacterium tuberculosis (Mtb), a slow-growing pathogen, at single-cell level. Here, use time-lapse imaging mathematical modeling characterize these fundamental properties Mtb. Whereas most organisms grow exponentially level, find that Mtb exhibits unique linear mode. characteristics are highly variable from cell-to-cell,...
It is known that the arnB (or pmrH) gene encoding uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase plays a critical role in colistin resistance Pseudomonas aeruginosa through addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to lipid A. In this study, we attempted obtain colistin-resistant mutant from an arnB-deleted exposure colistin.We constructed deletion (P5ΔarnB :: nptIII) colistin-susceptible strain (P5) by allelic replacement mutagenesis, and mutants were...
Abstract Antibiotics are a cornerstone of medicine, placing bacterial pathogens under intense pressure to evolve new survival mechanisms. Analysis 51,229 Mycobacterium tuberculosis (Mtb) clinical isolates identified an essential transcriptional regulator, Rv1830 ( here named resR) as frequent target positive (adaptive) selection. resR mutants do not demonstrate canonical drug resistance or tolerance but instead have significantly faster recovery after treatment across all antibiotics and...
We investigated the effect of toxin-antitoxin (TA) systems in blaCTX-M-15-bearing plasmids Klebsiella pneumoniae on persister formation. The formation rate was notably high transconjugants bearing TA system than no systems. Activation relA and spoT expression higher with Thus, may contribute to maintenance host survival via