A5102757077- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Protein Degradation and Inhibitors
- Peroxisome Proliferator-Activated Receptors
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Click Chemistry and Applications
- Additive Manufacturing and 3D Printing Technologies
- Cyclopropane Reaction Mechanisms
- Synthesis and Reactivity of Heterocycles
- Metabolomics and Mass Spectrometry Studies
- Carbon Nanotubes in Composites
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Porphyrin and Phthalocyanine Chemistry
- biodegradable polymer synthesis and properties
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- RNA modifications and cancer
- Molecular Sensors and Ion Detection
- Computational Drug Discovery Methods
- Photopolymerization techniques and applications
Harvard University
2018-2025
Massachusetts General Hospital
2018-2025
Center for Systems Biology
2018-2025
Myongji University
2025
Institute for Basic Science
2023-2024
Seoul National University
2012-2021
Dankook University
2016-2020
CHA University
2018
University of Seoul
2014-2016
Seoul Institute
2016
In obesity, adipocyte hypertrophy and proinflammatory responses are closely associated with the development of insulin resistance in adipose tissue. However, it is largely unknown whether per se might be sufficient to provoke obese Here, we demonstrate that lipid-overloaded hypertrophic adipocytes resistant independent inflammation. Treatment saturated or monounsaturated fatty acids resulted hypertrophy, but were observed only treated acids. Regardless inflammation, large unilocular lipid...
Blocking phosphorylation of peroxisome proliferator-activated receptor (PPAR)γ at Ser(273) is one the key mechanisms for antidiabetes drugs to target PPARγ. Using high-throughput screening, we here describe that Gleevec blocks cyclin-dependent kinase 5-mediated PPARγ devoid classical agonism as a antagonist ligand. In high fat-fed mice, improved insulin sensitivity without causing severe side effects associated with other PPARγ-targeting drugs. Furthermore, reduces lipogenic and...
Extracellular vesicles (EV) are shed by tumor cells but little is known about their individual molecular phenotypes and heterogeneity. While exosomes have received considerable attention, much less larger microvesicles. Here we profile single microvesicles (MV) from glioblastoma (GB) MV the plasma of patients.
Abstract Fluorescence imaging enables the uniquely sensitive observation of functional‐ and molecular‐recognition events in living cells. However, only a limited range biological processes have been subjected to because lack design strategy difficulties synthesis biosensors. Herein, we report facile emission‐tunable predictable Seoul‐Fluors, 9‐aryl‐1,2‐dihydrolopyrrolo[3,4‐ b ]indolizin‐3‐ones, with various R 1 2 substituents by coinage‐metal‐catalyzed intramolecular 1,3‐dipolar...
Purifying extracellular vesicles (EVs) from complex biological fluids is a critical step in analyzing EVs molecularly. Plasma lipoprotein particles (LPPs) are significant confounding factor as they outnumber >104 -fold. Given their overlap size, LPPs cannot be completely removed using standard size-exclusion chromatography. Density-based separation of can applied but impractical for routine use clinical research and practice. Here new approach, known dual-mode chromatography (DMC), capable...
Cancer extracellular vesicles (EVs) are highly heterogeneous, which impedes our understanding of their function as intercellular communication agents and biomarkers. To deconstruct this heterogeneity, we analyzed RNAs (exRNAs) proteins (exPTNs) from size fractionation large, medium, small EVs ribonucleoprotein complexes (RNPs) mouse glioblastoma cells by RNA sequencing quantitative proteomics. mRNA medium-sized most closely reflects the cellular transcriptome, whereas EV exRNA is enriched in...
Detecting early cancer through liquid biopsy is challenging due to the lack of specific biomarkers for lesions and potentially low levels these markers. The current study systematically develops an extracellular-vesicle (EV)-based test detection, specifically focusing on high-grade serous ovarian carcinoma (HGSOC). marker selection based emerging insights into HGSOC pathogenesis, notably that it arises from precursor within fallopian tube. This work thus establishes murine tube (mFT) cells...
We revealed the X-ray structure of PPARγ co-crystallized with SR1664 bound to alternate binding site and confirmed that this blocks phosphorylation Ser273.
Fluorescence microscopy is the method of choice in biology for its molecular specificity and super-resolution capabilities. However, it limited to a narrow z range around one observation plane. Here, we report an imaging approach that recovers full electric field fluorescent light with single-molecule sensitivity. We expand principle digital holography fast detection by eliminating need phase cycling enable three-dimensional (3D) tracking individual nanoparticles in-plane resolution 15 nm...
The early detection of neurodegenerative diseases necessitates the identification specific brain-derived biomolecules in peripheral blood. In this context, our investigation delineates role amyloid precursor-like protein 1 (APLP1)—a predominantly localized oligodendrocytes and neurons—as a previously unidentified biomarker extracellular vesicles (EVs). Through rigorous analysis, APLP1 + EVs from human sera were unequivocally determined to be cerebral origin. This assertion was corroborated...
<title>Abstract</title> In this study, characterization of surface enhanced infrared absorption (SEIRA) spectroscopy under attenuated total reflection (ATR) mode was provided to effectively identify irregularly shaped microplastics (MPs) through mid-IR plasmon and phonon resonance effect. Here, MPs specific binding microneedle array fabricated examined for the detection effects out aggregates or clusters gold nanorods (Au NRs), which were short-range-ordered among Au NRs spaced within...
The molecular charge of fluorescent bioprobes has recently received much attention due to its influence on cellular uptake. Herein, we demonstrate the effect glucose their GLUT-specific We also applied GB2-Cy3 in vivo imaging zebrafish model.
Pyrimidine-containing novel organic fluorophores were discovered and successively applied to monitor the lipid droplets in live cellular systems.
For the immediate detection of gaseous strong acids, it is advantageous to employ colorimetric textile sensors based on halochromic dyes. Thus, a rhodamine dye with superior pH sensitivity and high thermal stability was synthesized incorporated in nylon 6 polyester fabrics fabricate through dyeing printing methods. The spectral properties solubility were examined; acidic gas as well durability reversibility fabricated investigated. Both dyed printed exhibited reaction rate distinctive color...
Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools diagnosis and discovery of novel therapeutic agents via the development phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, cellular uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, found that net charge fluorochromes has considerable effects on uptake probe. Competition assay d/l-glucose well Western blot analysis...
Abstract Small‐molecule glucose uptake enhancers targeted to myotubes and adipocytes were developed through a phenotypic screening linked with target identification rational optimization. The protein of glucose‐uptake was identified as nuclear receptor PPARγ (peroxisome proliferator‐activated gamma). Subsequent optimization initial hits generated lead compounds high potency for transactivation cellular uptake. Finally, we confirmed that the chirality optimized ligands differentiates their...
Abstract The rational improvement of photophysical properties can be highly valuable for the discovery novel organic fluorophores. Using our new design strategy guided by oscillator strength, we developed a series full‐color‐tunable furoindolizine analogs with improved molar absorptivity through fusion furan ring into indolizine‐based Seoul fluorophore. excellent correlation between computable values (oscillator strength and theoretical S 0 –S 1 energy gap) (molar emission wavelength)...
Abstract Fluorescence imaging enables the uniquely sensitive observation of functional‐ and molecular‐recognition events in living cells. However, only a limited range biological processes have been subjected to because lack design strategy difficulties synthesis biosensors. Herein, we report facile emission‐tunable predictable Seoul‐Fluors, 9‐aryl‐1,2‐dihydrolopyrrolo[3,4‐ b ]indolizin‐3‐ones, with various R 1 2 substituents by coinage‐metal‐catalyzed intramolecular 1,3‐dipolar...
Optical sensing is one of the key-enablers modern diagnostics. Especially label-free imaging modalities hold great promise as they eliminate labeling procedures prior to analysis. However, scattering signals nanometric particles scale with their volume-square. This unfavorable scaling makes it extremely difficult quantitatively characterize intrinsically heterogeneous clinical samples, such extracellular vesicles, signal variation easily exceeds dynamic range currently available cameras....
Extracellular vesicles (EVs) are nanosized that have been garnering a lot of attention for their valuable role as potential diagnostic markers and therapeutic vehicles plethora pathologies. While EV from biofluids such plasma, serum, urine, cerebrospinal fluid, in vitro cell culture-based platforms extensively studied, significant knowledge gap remains is the characterization specific organ-derived EVs (ODE). Here, we present standardized protocol isolation purified isolated brain, heart,...