A5060047552- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Tuberculosis Research and Epidemiology
- Helicobacter pylori-related gastroenterology studies
- DNA and Nucleic Acid Chemistry
- Amino Acid Enzymes and Metabolism
- Porphyrin Metabolism and Disorders
- DNA Repair Mechanisms
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolites in Food Biotechnology
- Biochemical Acid Research Studies
- Photosynthetic Processes and Mechanisms
- Biochemical and Structural Characterization
- Protein Interaction Studies and Fluorescence Analysis
- Advanced biosensing and bioanalysis techniques
- Toxin Mechanisms and Immunotoxins
Seoul National University
2012-2021
National Cancer Center
2017
University of Seoul
2016
National Cancer Center
2010-2011
European Science Foundation
2011
Soongsil University
2011
Paul Scherrer Institute
2011
Deutsches Elektronen-Synchrotron DESY
2011
Yonsei University
2011
National Tsing Hua University
2011
Alpha-synuclein (alpha-SN) is a ubiquitous protein that especially abundant in the brain and has been postulated to play central role pathogenesis of Parkinson's disease, Alzheimer's other neurodegenerative disorders. However, little known about neuronal functions alpha-SN molecular cellular mechanisms underlying loss. Here, we show plays dual roles neuroprotection neurotoxicity depending on its concentration or level expression. At nanomolar concentrations, a-SN protected neurons against...
The intracellular pathogen Mycobacterium tuberculosis ( Mtb ) causes tuberculosis. Enhanced survival (Eis) protein, secreted by , enhances of smegmatis Msm in macrophages. Eis was shown to suppress host immune defenses negatively modulating autophagy, inflammation, and cell death through JNK-dependent inhibition reactive oxygen species (ROS) generation. recently demonstrated contribute drug resistance acetylating multiple amines aminoglycosides. However, the mechanism enhanced remains...
The crystal structure of the Fab galactan-binding immunoglobulin J539 (a mouse IgA,kappa) has been determined at a resolution approximately 2.6 A by X-ray diffraction. starting model was that obtained from real space search described previously (Navia, M.A., Segal, D.M., Padlan, E.A., Davies, D.R., Rao, D.N., Rudikoff, S. and Potter, M. "Crystal 4.5 resolution." Proc. Nat. Acad. Sci. USA, 76:4071-4074, 1979). This now refined restrained least-squares procedures to an R-value 19% for 11,690...
Abstract Shoot apical meristem (SAM) development is coordinately regulated by two interdependent signaling events: one maintaining stem cell identity and the other governing initiation of lateral organs from flanks SAM. The networks involved in this process are interconnected multiple molecular mechanisms. Class III homeodomain-leucine zipper (HD-ZIP III) proteins most extensively studied transcription factors regulation. However, how different signals integrated to maintain pattern organ...
The three-dimensional structure of ribulose-1,5-biphosphate carboxylase-oxygenase (RuBisCO), has been determined at 2.6 A resolution. This enzyme initiates photosynthesis by combining carbon dioxide with ribulose bisphosphate to form two molecules 3-phosphoglycerate. In plants, RuBisCO is built from eight large (L) and small (S) polypeptide chains, or subunits. Both S chains the NH2-terminal domain (N) L are antiparallel beta, "open-face-sandwich" domains four-stranded beta sheets flanking...
Abstract The surface‐enhanced Raman (SER) spectra of aromatic amino acids ( L ‐phenylalanine, ‐tyrosine and tryptophan) their glycyl dipeptides (glycyl‐ glycyl‐ ‐tryptophan) adsorbed on Ag colloidal particles have been obtained. ordinary show satisfactory correlations. most prominent features in the SER all compounds are strongly enhanced peaks at about 930 1390 cm −1 due to CCOO − stretching COO symmetric vibrational modes, respectively. This is interpreted as suggesting that above surface...
Human nucleophosmin (NPM; also known as B23, NO38, or numatrin), encoded by the NPM1 gene, is an abundant phosphoprotein in nucleoli.1-3 It primarily localized to nucleolus but has been shown shuttle between cytoplasm and nucleus.4 Two isoforms of NPM, designated B23.1 B23.2,5 consist 294 259 amino acid residues, respectively, with being prevalent form all tissues.5, 6 The C-terminal 35 acids are missing from B23.2 N-terminal 257 residues two splice variants identical. NPM participates...
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the nuclear superfamily. It functions as ligand-activated transcription factor and plays important roles in regulation adipocyte differentiation, insulin resistance, inflammation. Here, we report crystal structures PPARγ complex with lobeglitazone, novel agonist, rosiglitazone for comparison. The thiazolidinedione (TZD) moiety lobeglitazone occupies canonical ligand-binding pocket near activation function-2 (AF-2) helix...
Friedreich ataxia is an autosomal recessive neurodegenerative disease caused by defects in the FRDA gene, which encodes a mitochondrial protein called frataxin. Frataxin evolutionarily conserved, with homologs identified mammals, worms, yeast, and bacteria. The CyaY proteins of γ-purple bacteria are believed to be closely related ancestor In this study, we have determined crystal structure from Escherichia coli at 1.4-Å resolution. It reveals fold consisting six-stranded antiparallel β-sheet...
Abstract Surface‐enhanced Raman scattering (SERS) of adenine, adenosine, 2′‐deoxyadenosine, 5′‐dAMP, 5′‐rAMP, 5′‐rADP and 5′‐rATP adsorbed on aqueous Ag sol has been investigated. A shift the adenine ring breathing mode from 746 to 735 cm −1 with increasing concentration is interpreted as reflecting reorientation surface. Such a not observed for 2′‐deoxyadenosine 5′‐dAMP. SERS spectra can be obtained either at neutral pH in presence pyridine or by lowering sol. The effect discussed terms...
Difficulty in the treatment of tuberculosis and growing drug resistance Mycobacterium (Mtb) are a global health issue. Carbapenems inactivate L,D-transpeptidases; meropenem, when administered with clavulanate, showed vivo activity against extensively drug-resistant Mtb strains. LdtMt2 (Rv2518c), one two functional L,D-transpeptidases Mtb, is predominantly expressed over LdtMt1 (Rv0116c). Here, crystal structure N-terminally truncated (residues Leu131-Ala408) reported both ligand-free...
The N-acetyltransferase arrest defective 1 (ARD1) is an important regulator of cell growth and differentiation that has emerged recently as a critical molecule in cancer progression. However, the regulation enzymatic biological activities human ARD1 (hARD1) presently poorly understood. Here, we report hARD1 undergoes autoacetylation this modification essential for its functional activation. Using liquid chromatography-tandem mass spectrometry site-directed mutational analyses, identified...