A5045468569- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Blood properties and coagulation
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Helicobacter pylori-related gastroenterology studies
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- DNA Repair Mechanisms
- RNA Research and Splicing
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Protein Degradation and Inhibitors
- ATP Synthase and ATPases Research
- Galectins and Cancer Biology
- Protein Tyrosine Phosphatases
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
- Histone Deacetylase Inhibitors Research
- Multiple Myeloma Research and Treatments
- Cholinesterase and Neurodegenerative Diseases
National Cancer Center
2016-2025
Korea Advanced Institute of Science and Technology
2014-2020
Daejeon University
2015-2019
Government of the Republic of Korea
2015-2019
Advanced Institute of Convergence Technology
2014-2015
Dana-Farber Cancer Institute
2015
Korea Photonics Technology Institute
2012
Seoul National University
2000-2007
National Cancer Institute
2001
The proximal domains of mitochondria and the endoplasmic reticulum (ER) are linked by tethering factors on each membrane, allowing efficient transport substances, including lipids calcium, between them. However, little is known about regulation function mitochondria-ER contacts (MERCs) dynamics under mitochondrial damage. In this study, we apply NanoBiT technology to develop MERBiT system, which enables measurement reversible MERCs formation in living cells. Analysis using system suggests...
Early-onset gastric cancer, which develops in patients younger than most cancers, is usually detected at advanced stages, has diffuse histologic features, and occurs more frequently women. We investigated somatic genomic alterations associated with the unique characteristics of sporadic cancers (DGCs) from patients.We conducted whole exome RNA sequence analyses 80 resected DGC samples 45 years old or Korea. Patients pathogenic germline mutations CDH1, TP53, ATM were excluded onset this...
Abstract Glioblastoma is a highly malignant tumor that easily acquires resistance to treatment. The stem-cell-like character (stemness) has been thought be closely associated with the treatment of glioblastoma cells. In this study, we determined farnesyl diphosphate synthase (FDPS), key enzyme in isoprenoid biosynthesis, plays an important role maintaining stemness. A comparison mRNA expression patient-derived sphere cells, which maintain stemness, and their differentiated counterparts, lose...
Graphitic carbon nitride (g-C3N4) has a suppressive capability toward Alzheimer's Aβ aggregation under light-illumination. Photoinduced electrons of g-C3N4 generate reactive oxygen resulting in photooxidation amyloid peptides that blocks aggregation. Fe doping frameworks results enhanced optical properties and even stronger inhibition As service to our authors readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed may be re-organized...
Multifunctional carbon dots are synthesized to chelate Cu(<sc>ii</sc>) ions, suppress Alzheimer's β-amyloid (Aβ) aggregation, and photooxygenate Aβ peptides.
Abstract The VHL-containing cullin-RING E3 ubiquitin ligase (CRL2 VHL ) complex is an commonly used in targeted protein degradation (TPD). Hydroxyproline-based ligands that mimic substrates have been developed as anchor molecules for proteolysis-targeting chimeras (PROTACs) TPD. To expand the chemical space ligands, we conducted fragment screening using VHL–ELOB–ELOC (VBC) proteins. We found certain 7-hydroxycoumarin derivatives (7HCs), rather than VHL, would bind to ELOC component of VBC...
Human nucleophosmin (NPM; also known as B23, NO38, or numatrin), encoded by the NPM1 gene, is an abundant phosphoprotein in nucleoli.1-3 It primarily localized to nucleolus but has been shown shuttle between cytoplasm and nucleus.4 Two isoforms of NPM, designated B23.1 B23.2,5 consist 294 259 amino acid residues, respectively, with being prevalent form all tissues.5, 6 The C-terminal 35 acids are missing from B23.2 N-terminal 257 residues two splice variants identical. NPM participates...
The self‐assembly of amyloidogenic peptides into β‐sheet‐rich aggregates is a general feature many neurodegenerative diseases, including Alzheimer's disease, which signifies the need for effective attenuation amyloid aggregation toward alleviating amyloid‐associated neurotoxicity. This study reports that photoluminescent carbon nanodots (CDs) can effectively suppress β‐amyloid (Aβ) and function as β‐sheet breaker disintegrating preformed Aβ aggregates. synthesizes CDs using ammonium citrate...
Core–shell plasmonic nanohybrids are synthesized through a simple solutionbased process utilizing mussel-inspired polydopamine (PDA). The multi-purpose PDA not only facilitates metal formation, but also serves as scaffold to incorporate photosensitizers around the cores, well an adhesive between and substrate. resulting assembly exhibits highly enhanced light absorption in photo catalytic systems augment artificial photosynthesis. As service our authors readers, this journal provides...
Abnormal aggregation of β-amyloid (Aβ) peptides is a major hallmark Alzheimer's disease (AD). In spite numerous attempts to prevent the β-amyloidosis, no effective drugs for treating AD have been developed date. Among many candidate chemicals, methylene blue (MB) has proved its therapeutic potential in number vitro and vivo studies; but result recent clinical trials performed with MB derivative was negative. Here, aid multiple photochemical analyses, we first report that photoexcited...
The neuregulin 1 (NRG1) fusion is a recently identified novel driver oncogene in invasive mucinous adenocarcinoma of the lung (IMA). After identification case SLC3A2-NRG1 patient with IMA, we verified this gene cohort 59 patients IMA. Targeted cancer panel sequencing and RT-PCR possible coexistence other oncogenes. Among IMAs, found 16 NRG1 fusions (13 3 CD74-NRG1). Of fusions, concurrent KRAS codon 12 mutations were 10 cases. We also NRAS Q61L mutation EML4-ALK additional two cases fusions....
Antibodies to aquaporin-4 (AQP4-Ab), known as NMO-IgG, are a sensitive and specific marker for neuromyelitis optica (NMO).To develop an enzyme-linked immunosorbent assay (ELISA) AQP4-Ab, we expressed M23 isoform of human AQP4 in baculovirus system, used it antigen. We measured AQP4-Ab the sera 300 individuals: 64 with definite NMO, 31 high-risk 105 multiple sclerosis (MS), 57 other neurological diseases (ONDs), 43 healthy controls. also performed longitudinal measurements 787 samples...
Abstract API5 (APoptosis Inhibitor 5) and nuclear FGF2 (Fibroblast Growth Factor 2) are upregulated in various human cancers correlated with poor prognosis. Although their physical interaction has been identified, the function related to resulting complex is unknown. Here, we determined crystal structure of API5–FGF2 identified critical residues driving protein interaction. These findings provided a structural basis for localization isoform lacking canonical signal cryptic sequence FGF2. The...
There has been considerable interest in virulence genes the plasticity region of Helicobacter pylori , but little is known about many these genes. JHP940, one factors encoded by H. strain J99, a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well enhanced translocation NF-κB cultured macrophages. Here we have characterized structure function JHP940 to provide framework for better understanding its role inflammation . Our work demonstrates...