A5044752551- Lipid metabolism and biosynthesis
- Click Chemistry and Applications
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Chemical Synthesis and Analysis
- Adipose Tissue and Metabolism
- 14-3-3 protein interactions
- Metabolomics and Mass Spectrometry Studies
- Nanoplatforms for cancer theranostics
- Computational Drug Discovery Methods
- Synthesis of Indole Derivatives
- Biotin and Related Studies
- Sphingolipid Metabolism and Signaling
- Advanced Fluorescence Microscopy Techniques
- Nerve injury and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Photochromic and Fluorescence Chemistry
- Cell death mechanisms and regulation
Seoul National University
2016-2023
Seoul Institute
2016
A label-free method for proteome-wide target identification was developed using in-gel fluorescence difference caused by thermal stability shift.
Pyrimidine-containing novel organic fluorophores were discovered and successively applied to monitor the lipid droplets in live cellular systems.
Tau protein aggregates are a recognized neuropathological feature in Alzheimer's disease as well many other neurodegenerative disorders, known tauopathies. The development of tau-targeting therapies is therefore extremely important but efficient strategies or targets still unclear. Here, we performed cell-based phenotypic screening under endoplasmic reticulum (ER) stress conditions and identified small molecule, SB1617, capable suppressing abnormal tau aggregation. By applying label-free...
In situ conjugation of fluorescent molecules to biomolecules such as proteins under spatiotemporal control offers a powerful means for studying biological systems. For that purpose, the o-quinone methide chemistry involving sequence trigger–release–conjugation (TRC) process provides versatile method. We have developed new TRC platform bearing quaternary ammonium salt release process, which can be structurally modified and readily synthesized from commonly used aryl alcohol-type organic...
Abstract Lipid droplets (LDs) are involved in various biological events cells along with their primary role as a storage center for neutral lipids. Excessive accumulation of LDs is highly correlated diseases, including metabolic diseases. Therefore, basic understanding the molecular mechanism LD degradation would be beneficial both academic and industrial research. Lipophagy, selective autophagy mechanism/LD process, has gained increased attention research community. Herein, we sought to...
Abstract The highly cytotoxic marine natural product callyspongiolide holds great promise as a warhead of antibody-drug conjugate in cancer therapeutics; however, the mechanism underlying its cytotoxicity remains unclear. To elucidate how kills cells, we employed label-free target identification with thermal stability-shift-based fluorescence difference two-dimensional (2-D) gel electrophoresis (TS-FITGE), which allowed observation unique phenomenon protein-spot separation on 2-D gels upon...
Obesity has become a pandemic that threatens the quality of life and discovering novel therapeutic agents can reverse obesity obesity-related metabolic disorders are necessary. Here, we aimed to identify new anti-obesity using phenotype-based approach. We performed image-based high-content screening with fluorogenic bioprobe (SF44), which visualizes cellular lipid droplets (LDs), initial hit compounds. A structure-activity relationship study led us yield bioactive compound SB1501, reduces...
Abstract Tau protein aggregates are a recognized neuropathological feature in Alzheimer's disease as well many other neurodegenerative disorders, known tauopathies. The development of tau‐targeting therapies is therefore extremely important but efficient strategies or targets still unclear. Here, we performed cell‐based phenotypic screening under endoplasmic reticulum (ER) stress conditions and identified small molecule, SB1617, capable suppressing abnormal tau aggregation. By applying...
Neurodegenerative Erkrankungen sind durch die Ansammlung von fehlgefalteten Proteinen wie dem Tau-Protein gekennzeichnet. In ihrem Forschungsartikel auf S. 1859 führen Seung Bum Park et al. ein zellbasiertes phänotypisches Screening und identifizieren kleines Molekül, SB1617, das in der Lage ist, anomale Aggregation des Tau-Proteins unter ER-Stress zu unterdrücken. Die markierungsfreie Target-Identifizierung Studien zur Wirkungsweise zeigen, dass Behandlung mit SB1617 Wiederherstellung...