A5019275397- Molecular Sensors and Ion Detection
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Photochromic and Fluorescence Chemistry
- Sulfur Compounds in Biology
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Lipid metabolism and biosynthesis
- Synthesis of Indole Derivatives
- Photochemistry and Electron Transfer Studies
- Porphyrin and Phthalocyanine Chemistry
- Advanced Fluorescence Microscopy Techniques
- Metabolomics and Mass Spectrometry Studies
- Adenosine and Purinergic Signaling
- Graphene and Nanomaterials Applications
- Lanthanide and Transition Metal Complexes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Carbon and Quantum Dots Applications
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Cancer, Hypoxia, and Metabolism
- Photoreceptor and optogenetics research
Pohang University of Science and Technology
2017-2024
Korea Post
2020
Government of the Republic of Korea
2017-2020
Cheongam College
2018
Indian Institute of Technology Kharagpur
1989
Indian Association for the Cultivation of Science
1979-1984
Abstract Fluorescence monitoring of ATP in different organelles is now feasible with a few biosensors developed, which, however, show low sensitivity, limited biocompatibility, and accessibility. Small‐molecule probes that alleviate those limitations thus have received much attention recently, leading to target several except for the nucleus. We disclose first small‐molecule probe selectively detects nuclear through reversible binding, 25‐fold fluorescence enhancement at pH 7.4 excellent...
A fluorescent probe enables two-photon ratiometric imaging of endogenous hypochlorous acid, a reactive oxygen species, in cells and tissues.
Hypoxia, a condition of oxygen deficiency in tissues, features various diseases including solid tumor. Under hypoxia, several reductases such as nitroreductases are elevated. Based on this fact, we have investigated an indirect way to assess the hypoxia susceptibility different organ tissues (mouse lung, heart, spleen, kidney, and liver) by detecting nitroreductase present within. Among organs, kidney showed notable which was due renal medulla, not cortex, observed ratiometric fluorescence...
Dynamic tracking of cancer cells with two-photon excitable polyphenolic carbon dots superior tumor targeting properties.
Lipid droplets (LDs) act as an energy reservoir in cancer cells; on the other hand, mitochondria are hyperactive to fulfill demand accelerate cell proliferation. We interested unfolding relationship between cellular and producer through fluorescence labeling. Thus, a dual organelle-targeted fluorescent probe MLD-1 has been rationally developed. It visualized crosstalk mitochondrial dysfunction fluctuation of LDs live cells. Its two-photon ability allowed us acquire deep tissue images. For...
Elastase, a serine protease, plays important roles in our body food digestion and defence against pathogens. Particularly, the elastase present neutrophils is directly associated with inflammatory bowel disease (IBD). Through rational approach, we have developed fluorescent probe that has multiple advantages for biological applications including two-photon ratiometric imaging capability. Using probe, which capable of detecting intracellular activity inflammation, investigated level changes...
γ-Glutamyltransferase (GGT) is involved in maintaining the intracellular glutathione levels and, at its elevated levels, associated with various diseases including cancer and myocardial infarction. To study this enzyme biological systems, fluorescent probes have received significant attention recently. As fluorescence signal sensitive to environmental fluctuations; however, it challenging address fluctuation issue. Disclosed benzocoumarin-based probe that enables ratiometric imaging of GGT...
Abstract Adenosine triphosphate (ATP), as the main energy source in cells, powers most cellular activities, such synthesis and transportation of biomolecules, cell signaling, muscle contraction. Therefore, detection monitoring ATP are essential to understand many biological processes, demanding molecular probes with high selectivity sensitivity, fast response times, along long‐term tissue imaging capability. We overviewed reported small‐molecule fluorescent for by categorizing their...
An 8-hydroxybenzo[<italic>g</italic>]coumarin derivative with two-photon absorptivity and a large Stokes shift has been highlighted as an environment insensitive dye platform.
Human serum albumin exerts multifunctions, such as maintaining the oncotic pressure of plasma, carrying hydrophobic molecules, and acting most important antioxidant in blood. Lower levels are linked to several cardiovascular diseases, dysfunction reabsorption kidney is liver disease, renal disorder, diabetes. Albumin thus a powerful diagnostic prognostic marker; however, its quantification urine by readily affordable tools challenging owing very low concentration. To address this issue, we...
Mitigating the adverse effects of anticancer agents requires innovative prodrug engineering. In this study, we showcase potential our o-quinone methide-based trigger-release-conjugation platform as a versatile tool for constructing advanced systems. Using platform, achieved light-triggered release an drug mechlorethamine, targeting mitochondrial DNA. The entire process was adeptly tracked through emission fluorescence signals, revealing notable across various cancer cell lines compared to...
The benzocoumarins show distinctive emission behaviour from some commonly-used dyes in organic, aqueous buffer, and cellular media, which compels us to recognize the environment as third space for fluorophores.
Abstract Small‐molecule dyes that emit blue fluorescence, particularly those in the S‐blue region, are limited number. Functionalization of benzene with electron‐donating and ‐accepting groups generates single‐benzene fluorophores (SBFs) tunable emission properties. By exploring unprecedented, interactive twin intramolecular H‐bonds, a novel type SBFs exhibits several notable features for bioimaging applications is developed. Specifically, aniline derivatives bearing two carbonyl/carboxyl at...
Lipid droplets (LDs), the essential cytosolic fat storage organelles, have emerged as pivotal regulators of cellular metabolism and are implicated in various diseases. The noninvasive monitoring LDs necessitates fluorescent probes with precise organelle selectivity biocompatibility. Addressing this need, we engineered a probe by strategically modifying structure conventional two-photon-absorbing dipolar dye, acedan. This innovative approach induces nanoaggregate formation aqueous...
NAD(P)H quinone oxidoreductase-1 (NQO1), a protective enzyme against cellular oxidative stress, is expressed abnormally high in solid tumors and thus recognized as cancer biomarker. To develop fluorescent NQO1 probe with practicality, we investigated benzo-rosol fluorophores linked known self-immolative substrate. Four candidates exhibited ratiometric sensing behavior toward the enzyme, satisfying our orbital mismatch stratagem proposed before, under dual-excitation dual-emission conditions...
Near-infrared-emitting hemicyanine dyes are widely used in activatable fluorescent probes for various biological analytes; however, they chemically unstable and show photoinstability, as shown here with naphthalene-based hemicyanines containing a typical moiety, 2-indolium. These issues can be resolved 4-pyridinium derivative, which also has good two-photon imaging capability.
Abstract Water‐soluble, quadrupolar (A‐π‐D‐π‐A) pyrrolo[3,2‐ b ]pyrrole dyes have been prepared, and their photophysical properties, including solid‐state fluorescence, are characterized. Analysis of packing patterns the para analogue in single crystal shows negligible π–π interactions between molecular planes stacked layers, resulting no fluorescence quenching aggregate state. One is used for imaging cells by confocal two‐photon microscopy, showing potential applicability such as bioimaging agents.
In situ conjugation of fluorescent molecules to biomolecules such as proteins under spatiotemporal control offers a powerful means for studying biological systems. For that purpose, the o-quinone methide chemistry involving sequence trigger–release–conjugation (TRC) process provides versatile method. We have developed new TRC platform bearing quaternary ammonium salt release process, which can be structurally modified and readily synthesized from commonly used aryl alcohol-type organic...
Fluorescent probes bearing a reactive moiety of 1,1-dicyanovinyl are known to detect several biological species including bisulfite and hypochlorous acid, which, however, possess selectivity issue among those analytes. Structural modifications the group for optimal steric electron effects based on theoretical calculations led us address issue, offering new moieties that provide complete analyte selectivity, between in cells as well solution.
The results of a comparative study the absorption and emission properties triplet yields isomeric cyanopyridines (CNP) benzonitrile under various environmental conditions are reported. All three CNP isomers found to be nonfluorescent but exhibit fairly strong phosphorescence π, π character in rigid polar media at 77 K. ΦP values sensitively dependent on rigidity (or viscosity) crystalline structure solvent medium. Whereas 3-CNP molecule phosphoresces with moderately intensity hydrocarbon...