A5051273619- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Bacterial Infections and Vaccines
- Gut microbiota and health
- RNA Interference and Gene Delivery
- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Inflammasome and immune disorders
- Acne and Rosacea Treatments and Effects
- Cancer Genomics and Diagnostics
- Mosquito-borne diseases and control
- Analytical Chemistry and Sensors
- Antimicrobial Peptides and Activities
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Molecular Biology Research
- Barrier Structure and Function Studies
- Carbon and Quantum Dots Applications
- Advanced Nanomaterials in Catalysis
- Microbial metabolism and enzyme function
- Biofuel production and bioconversion
- Photochromic and Fluorescence Chemistry
- Plant nutrient uptake and metabolism
Pohang University of Science and Technology
2014-2023
Gwangju Institute of Science and Technology
2020
Government of the Republic of Korea
2018-2020
Korea Post
2020
The gut microbiota has an important role in the barrier, inflammation and metabolic functions. Studies have identified a close association between intestinal barrier diseases, including obesity type 2 diabetes (T2D). Recently, Akkermansia muciniphila been reported as beneficial bacterium that reduces disruption insulin resistance. Here we evaluated of A. muciniphila-derived extracellular vesicles (AmEVs) regulation permeability. We found there are more AmEVs fecal samples healthy controls...
Gut microbiota play an important part in the pathogenesis of mucosal inflammation, such as inflammatory bowel disease (IBD). However, owing to complexity gut microbiota, our understanding roles commensal and pathogenic bacteria maintenance immune homeostasis is evolving only slowly. Here, we evaluated role their secreting extracellular vesicles (EV) development inflammation gut. Experimental IBD model was established by oral application dextran sulfate sodium (DSS) C57BL/6 mice. The...
Abstract Gut microbes might influence host metabolic homeostasis and contribute to the pathogenesis of type 2 diabetes (T2D), which is characterized by insulin resistance. Bacteria-derived extracellular vesicles (EVs) have been suggested be important in diseases once believed non-infectious. Here, we hypothesize that gut microbe-derived EVs are T2D. In vivo administration stool from high fat diet (HFD)-fed mice induced resistance glucose intolerance compared regular (RD)-fed mice....
Staphylococcus aureus is an important pathogenic bacterium that causes various infectious diseases. Extracellular vesicles (EVs) released from S. contain bacterial proteins, nucleic acids, and lipids. These EVs can induce immune responses leading to similar symptoms as during staphylococcal infection condition have the potential vaccination agent. Here, we show active immunization (vaccination) with aureus-derived adaptive immunity of antibody T cell responses. In addition, these vaccine...
Abstract The development of a multiplexed sensing platform is necessary for highly selective, sensitive, and rapid screening specific antibiotics. In this study, we designed novel multiplex aptasensor antibiotics by fluorescence resonance energy transfer (FRET) strategy using DNase I-assisted cyclic enzymatic signal amplification (CESA) method combined with aptamer/graphene oxide complex. aptamers sulfadimethoxine, kanamycin, ampicillin were conjugated Cyanine 3 (Cy3), 6-Carboxyfluorescein...
Extracellular vesicle (EV) has been reported to conduct critical pathophysiological functions as an emerging mode of communication in bacteria. Recently, Propionibacterium acnes, anaerobic Gram-positive human commensal found the skin and gastrointestinal tract, drawn increasing attention underestimated pathogen a variety diseases.For comprehensive understanding P. here we report isolation acnes EVs for first time identification 252 vesicular proteins with high confidence using triplicate...
The benzocoumarins show distinctive emission behaviour from some commonly-used dyes in organic, aqueous buffer, and cellular media, which compels us to recognize the environment as third space for fluorophores.
Propionibacterium acnes is a lipophilic commensal bacterium mainly found on the skin and in gastrointestinal tract. Pathophysiological effects of P. have recently been reported not only acne progression but various diseases. As an emerging mode bacterial communication, extracellular vesicles (EVs) demonstrated to conduct critical pathophysiological functions. To provide information lipid composition for first time, we conducted comparative lipidomic analysis EVs identified 214 lipids with...
Cell-free DNA (cfDNA) analysis, specifically circulating tumor (ctDNA) provides enormous opportunities for noninvasive early assessment of cancers. To date, PCR-based methods have led this field. However, the limited sensitivity/specificity necessitates search new methods. Here, we describe a direct approach to detect KRAS G12D mutated genes in clinical ctDNA samples with utmost LOD and sensitivity/specificity. In study, MutS protein was immobilized on tip an atomic force microscope (AFM),...
Polymyxin B resistance protein D (PmrD) plays a key role in the polymyxin B-resistance pathway, as it is signaling that can act specific connecter between PmrA/PmrB and PhoP/PhoQ. We conducted structural analysis to characterize Escherichia coli (E. coli) PmrD, which exhibits different features compared with PmrD other bacteria. The X-ray crystal structure of E. was determined at 2.00 Å resolution, revealing novel information such unambiguous secondary structures presence disulfide bond....
Abstract The soluble interleukin‐2 receptor α (sIL‐2Rα) is a broad indicator of clinical disease activity in various inflammatory diseases. Here we have developed, for the first time, rapid, washing‐free colorimetric aptasensor based on sIL‐2Rα aptamer ( K d =1.33 n m ). was fabricated with Au nanoparticles (AuNPs) adsorbing aptamers. On addition sIL‐2Rα, aptamers become desorbed from AuNPs, and this turn weakens absorption corresponding to AuNP‐catalyzed oxidation ortho ‐phenylenediamine o...
We demonstrate for the first time a fast aptamer generation method based on screen-printed electrodynamic microfluidic channel device, where specific selectively binds to target protein walls, following recovery and separation. A malaria as model target, Plasmodium vivax lactate dehydrogenase (PvLDH) was covalently bonded conductive polymer layer formed carbon walls react with DNA library in fluid. Then, AC electric field symmetrically applied inducing binding of molecules. In this case,...
As a counterpart to antibody-drug conjugates (ADCs), aptamer-drug (ApDCs) have been considered promising strategy for targeted therapy due the various benefits of aptamers. However, an aptamer merely serves as targeting ligand in ApDCs, whereas antibody enables unexpected therapeutic efficacy ADCs through antibody-dependent cellular cytotoxicity (ADCC). In this study, we developed tumor-specific with effector function and used it confirm feasibility more potent ApDCs. First, designed...
Polymyxin B resistance protein D (PmrD) plays a key role in the polymyxin B-resistance pathway, as it is signaling that can act specific connecter between PmrA/PmrB and PhoP/PhoQ. We conducted structural analysis to characterize Escherichia coli (E. coli) PmrD, which exhibits different features compared with PmrD other bacteria. The X-ray crystal structure of E. was determined at 2.00 Å resolution, revealing novel information such unambiguous secondary structures presence disulfide bond....