Polyphenol functionalized silver nanoparticles (AgNPs) have been developed and demonstrated as colorimetric sensors for the selective detection of gadolinium. The newly obtained AgNP-Gd3+ conjugates exhibit high aqueous dispersibility excitation dependent fluorescence emission. offer multicolor bioimaging potential owing to their excellent luminescence properties.

Vascular endothelial growth factor (VEGF) is considered as one of the vital factors for angiogenesis, which primarily responsible progress and maintenance new vascular network in tumor. Numerous studies report that inhibition VEGF-induced angiogenesis a potent technique cancer suppression. Recently, RNA interference, especially small interfering (siRNA) signified promising approach to suppress gene expression. However, clinical implementation biological macromolecules such siRNA...

RNA interference shows a great strategy for biological studies; however, delivering of small interfering (siRNA) remains challenging. Although several delivery vehicles, including cell-penetrating peptides, have been developed, their implementation is often restricted because endosomal entrapment. Herein, we report the formation self-assembled nanostructures from rationally designed cyclic peptides and explore them efficient functional biomacromolecules such as siRNA into mammalian cells....

Locked nucleic acid (LNA) is a conformationally restricted analogue, which potentially better alternative than DNA for application in the based biosensor technologies, due to its efficient and sequence-specific DNA/RNA detection capability lack of molecule–surface interaction on solid surfaces, compared DNA. We report, first time, straightforward way (based simple immersion method) generating an ordered self-assembled LNA monolayer, bioactive, onto gold(111) surface. This layer capable...

Cell-free DNA (cfDNA) analysis, specifically circulating tumor (ctDNA) provides enormous opportunities for noninvasive early assessment of cancers. To date, PCR-based methods have led this field. However, the limited sensitivity/specificity necessitates search new methods. Here, we describe a direct approach to detect KRAS G12D mutated genes in clinical ctDNA samples with utmost LOD and sensitivity/specificity. In study, MutS protein was immobilized on tip an atomic force microscope (AFM),...

In this work, a strategy for enhancing sensitivity in label-free DNA detection assay, where the basic operational principle involves of net surface stress induced bending motion piezoresistive microcantilever, upon target-binding, has been presented. A microcantilever array that allows experiments using sensor-reference configuration employed, cantilevers have functionalized by inkjet printing technology, short nucleic acid sequences similar length (here, 12-mer), on both sensor and...

In this work, we have explored an approach to finding a correlation between the mechanical response of metalloprotein against range applied force (by curve analysis) and its electrical under pressure stimulation current sensing atomic spectroscopy) at nanoscale. Iron-storage protein ferritin has been chosen as experimental model system because it naturally contains semiconducting iron core. This core consists large number atoms is therefore expected exert clear influence on overall...

Several investigations on DNA-based nucleic acid sensors performed in the past few years point toward requirement of an alternative that can detect target DNA strands more efficiently, i.e., with higher sensitivity and selectivity, be robust compared to sensor probes. Locked (LNA), a conformationally restricted analogue, is potentially better than DNA, since it nuclease-resistant, form stable duplex sequence-specific manner, interacts less substrate surface due presence rigid backbone. In...

Molecular monitoring is indispensable for the clinical management of chronic myeloid leukemia (CML) patients. Real-time quantitative polymerase chain reaction (RT-qPCR) gold standard assessment BCR-ABL transcript levels, which are critical in decision-making. However, frequent recurrence disease after drug discontinuation 60% patients has necessitated more sensitive and specific techniques to detect residual transcripts. Here, we describe a quantification method detection targets at very low...

Reliable quantification of binding affinity is important in biotechnology and pharmacology increasingly coupled with a demand for ultrasensitivity, nanoscale resolution, minute sample amounts. Standard techniques are not able to meet these criteria. This study provides new platform based on atomic force microscopy (AFM)-derived recognition imaging determine by visualizing single molecular bindings nanosize dendrons. Using DNA hybridization as demonstrator, an AFM sensor adorned cognate...

So far, there has been no report on molecularly resolved discrimination of single nucleobase mismatches using surface-confined stranded locked nucleic acid (ssLNA) probes. Herein, it is exemplified a label-independent force-sensing approach that an optimal coverage 12-mer ssLNA sensor probes formed onto gold(111) surface allows recognition ssDNA targets with twice stronger force sensitivity than The distributions are reproducible and the molecule-by-molecule measurements largely in agreement...

There has been a search for alternative nucleic acids that can be more effectively used in acid detection technologies compared to the DNA probes. Peptide (PNA), which contains non-ionic peptidic backbone, offers such possibilities since it is nuclease-resistant, binds with high affinity, and readily self-assembled onto solid substrates, e.g., gold(111), molecular backbone orientation away from substrate. Although application of PNA as sensor probe exemplified, so far there little or no...

Unambiguous identification of single base mismatches in nucleic acid sequences is great importance detection assays. However, ambiguities are often encountered with, and therefore, a strategy for attaining substantially large enhancement mismatch discrimination has been worked upon this study. Short single-stranded peptide (PNA) deoxyribonucleic (DNA) sensor probes that immobilized onto gold nanoparticle (AuNP) modified Au(111) surface have applied target DNA detection. It will be shown...

The recent emergence of on-surface LNA-based assays as potentially better alternatives over DNA-based approaches, due to enhanced sensitivity and target specificity, raises the need for precise identification factors that control performance these assays. In this work, we investigated whether probe density fully modified ssLNA probes on gold(111) surface could influence recognition capacity LNA sensing layer illustrated simple means it, primarily by adjusting salt concentration, nature...

Molecularly resolved, label-free discrimination of different types single nucleobase mismatches by LNA probes.

Challenges in reliable nucleic acid detection are manifold. The major ones related to false positive or negative signals due a lack of target specificity and low sensitivity, especially when plethora background sequences present that can mask the specific recognition signal. Utilizing designed synthetic acids commonly called xeno could offer potential routes meeting such challenges. In this article, we general framework detection, for nanoscale applications, discuss how why be truly an...

Floating hip with dislocation is a very high-energy, devastating, and rare injury whose treatment challenging, the outcome usually poor.

This study presents a mild, one-pot synthetic approach for the synthesis of multicolor, water soluble, photo luminescent CdS and CdSe quantum dots (QDs). To achieve this goal, cyclic peptides containing cysteine residues are rationally designed synthesized. Among tested, those two exhibit superior stabilizing properties, ensuring solubility long-term stability QDs in aqueous solutions several months. The newly synthesized unique excitation-dependent multicolor photoluminescence with yield...