A5020616161- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Nanofabrication and Lithography Techniques
- Dendrimers and Hyperbranched Polymers
- Organometallic Complex Synthesis and Catalysis
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Lanthanide and Transition Metal Complexes
- Monoclonal and Polyclonal Antibodies Research
- Semiconductor materials and devices
- Mechanical and Optical Resonators
- Radioactive element chemistry and processing
- Near-Field Optical Microscopy
- Gene expression and cancer classification
- Supramolecular Self-Assembly in Materials
- Integrated Circuits and Semiconductor Failure Analysis
- Analytical Chemistry and Sensors
- Cellular Mechanics and Interactions
- Graphene research and applications
- Chemical Synthesis and Analysis
- Inorganic and Organometallic Chemistry
- Luminescence and Fluorescent Materials
- Magnetism in coordination complexes
Pohang University of Science and Technology
2015-2024
Yonsei University
2007-2022
Institute for Basic Science
2017
Government of the Republic of Korea
2015-2016
Kyung Hee University
2009-2010
University of Nottingham
2007-2009
Washington State University
2008
Catholic University of Korea
2005
Korea University Medical Center
2005
Hongik University
2003
Vast numbers of studies and developments in the nanotechnology area have been conducted many nanomaterials utilized to detect cancers at early stages. Nanomaterials unique physical, optical electrical properties that proven be very useful sensing. Quantum dots, gold nanoparticles, magnetic carbon nanotubes, nanowires other materials developed over years, alongside discovery a wide range biomarkers lower detection limit cancer biomarkers. Proteins, antibody fragments, DNA RNA fragments are...
The surface of a fused silica and oxidized silicon wafer (SiO2/Si(100)) was treated with (4-aminophenyl)trimethoxysilane (1), (3-aminopropyl)triethoxysilane (2), (3-aminopropyl)diethoxymethylsilane (3), (3-aminopropyl)ethoxydimethylsilane (4) in solution. thickness thus formed aminosilane layers determined ellipsometry. In most cases silane coupling agents produce monolayers 6−10 Å thickness, but reagent 2 gives multilayers variable (6−100 Å) depending upon the dipping time. were allowed to...
Exosomes, small extracellular vesicles of endosomal origin, have been suggested to be involved in both the metabolism and aggregation Alzheimer's disease (AD)-associated amyloid β-protein (Aβ). Despite their ubiquitous presence inclusion components which can potentially interact with Aβ, role exosomes regulating synaptic dysfunction induced by Aβ has not explored. We here provide vivo evidence that derived from N2a cells or human cerebrospinal fluid abrogate synaptic-plasticity-disrupting...
The surface of a fused silica and oxidized silicon wafer (SiO2/Si(100)) was treated with (3-aminopropyl)triethoxysilane (1), (3-aminopropyl)diethoxymethylsilane (2), (3-aminopropyl)ethoxydimethylsilane (3) for 72 h in solution. Thickness the aminosilylated film out 1 increases rapidly, it turns to be around 100 Å h. Rather slow increase thickness is observed 2, which produces layers 8 14 10 h, respectively. reagent 3 monolayers constant 7−8 during whole span reaction. aminosilane layer...
There has been enormous interest in understanding and utilizing plasmon-enhanced fluorescence (PEF) with metal nanostructures, but maximizing the enhancement a reproducible, quantitative manner while reliably controlling distance between dyes particle surface for practical applications is highly challenging. Here, we designed synthesized fluorescence-amplified nanocuboids (FANCs) enhanced controlled PEF signals, fluorescent silica shell-coated FANCs (FS-FANCs) were then formed to fixate dye...
Four kinds of aminosilane layers on glass slides or silicon wafers were prepared. The amine densities the prepared with aminopropyldiethoxymethylsilane (APDES), aminopropylmonoethoxydimethylsilane (APMES), a mixture (aminopropyl)triethoxysilane (APTES) and n-butyltrimethoxysilane (n-BTMS) (v/v = 1:10) are 4.0(±0.8), 1.0(±0.2), 0.30(±0.6) amine/nm2, respectively. A substrate much higher density, that is, 40(±8) amines/nm2 was also by allowing aziridine to polymerize APDES-treated substrate....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure and reactivity of titanium-platinum -palladium heterobinuclear complexes with .mu.-methylene ligandsFumiyuki Ozawa, Joon Won Park, Peter B. Mackenzie, William P. Schaefer, Lawrence M. Henling, Robert H. GrubbsCite this: J. Am. Chem. Soc. 1989, 111, 4, 1319–1327Publication Date (Print):February 1, 1989Publication History Published online1 May 2002Published inissue 1 February...
MicroRNAs (miRNAs) play critical roles in controlling various cellular processes, and the expression levels of individual miRNAs can be considerably altered pathological conditions such as cancer. Accurate quantification miRNA at single-cell level will lead to a better understanding function. Here, we present direct sensitive method for detection using atomic force microscopy (AFM). A hybrid binding domain (HBD)-tethered tip enabled mature miRNAs, but not premature located individually on an...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-hydrogen bond activation through a binuclear carbon-hydrogen complexJoon Won. Park, Peter B. Mackenzie, William P. Schaefer, and Robert H. GrubbsCite this: J. Am. Chem. Soc. 1986, 108, 20, 6402–6404Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://doi.org/10.1021/ja00280a054RIGHTS & PERMISSIONSArticle Views296Altmetric-Citations69LEARN ABOUT THESE METRICSArticle...
To overcome the current limit of tip-enhanced spectroscopy that is based on metallic nano-probes, we developed a new scanning probe with nanostar, nanoparticle sharp spikes. A Au 5 nm was first attached to end tip through DNA-DNA hybridization and mechanical pick-up. The converted nanostar core diameter ∼70 spike lengths between 50 80 reduction Au(3+) ascorbic acid in presence Ag(+). Fabrication yields such tips exceeded 60%, more than 80% showed durability sufficient for use microscopy....
Structure and chemical properties of zirconium(IV) complexes containing a rigid naphthalene diamide ligand ([1,8-C10H6(NSiMe3)2]2-) or flexible propylene ligands ([1,3-C3H6(NR)2]2-, R = SiMe3, Si(i-Pr)3) are described. The reaction 1,8-C10H6(NHSiMe3)2 with Zr(NMe2)4 in refluxing toluene yields [1,8-C10H6(NSiMe3)2]Zr(NMe2)2 (1), the product 1 is converted to [1,8-C10H6(NSiMe3)2]ZrCl2 (2) moderate isolation yield by treatment excess ClSiMe3. X-ray crystallographic analysis establishes that 2...
The reaction of 1,8-diaminonaphthalene with trimethylchlorosilane and tri(isopropyl)chlorosilane gives N,N'-disilylated products (1a,b) in moderate yields. Double deprotonation 1a,b n-BuLi yields the dilithiated (2a,b) 78−85% yield. 2a,b TiCl4 produces diamidotitanium(IV) dichlorides, {1,8-C10H6(NSiMe3)2}TiCl2 (3a) {1,8-C10H6(NSi(i-Pr)3)2}TiCl2 (3b). crystal structure 3a shows that complex has an unusually large Cl−Ti−Cl angle (120.5°). Meanwhile, bulkier substituents on nitrogen atoms 3b...
Single-molecule force measurement methods have attracted increasing interest over recent years for the development of novel approaches biomolecular screening. However, many these developments are currently hindered by available biomolecule surface attachment methods, in that it is still not trivial to create surfaces and devices with highly defined functionality and/or uniformity. Here we offer a new approach address such issues based on formation dendron arrays. Through forces between...
In eukaryotic cells, organellar proteome biogenesis is pivotal for cellular function. Chloroplasts contain a complex proteome, the of which includes post-translational import nuclear-encoded proteins. However, mechanisms determining when and how nascent chloroplast-targeted proteins are sorted in cytosol unknown. Here, we establish timing mode interaction between ankyrin repeat-containing protein 2 (AKR2A), cytosolic targeting factor chloroplast outer membrane (COM) proteins, its interacting...
Significance Although synaptic plasticity is intimately controlled by synthesis of transmitter receptors and regulatory proteins, it remains largely unknown how distribution proteins could be modulated at the specific synapses. microRNAs (miRNAs) such as miR-134 adjust synapse development due to their functions for a distinct set proteins. We developed an imaging method in situ mediated atomic force microscopy (AFM), which revealed that miR-134s are enriched nearby dendritic shafts necks...
A single-molecule ferritin picking-up process was realized with the use of AFM, which enhanced by employing controlled dendron surface chemistry. The approach enabled placement a single protein molecule at very end an AFM tip. When used for magnetic force microscopy (MFM) imaging, tips were able to detect interactions approximately 10 nm sized nanoparticles. tip also showed characteristics "multifunctional" MFM probe that can sense from materials as well biomolecular interaction DNAs on...
The mapping capability of atomic force microscopy (AFM) enabled us to see captured prostate-specific antigens (PSAs) on a spot microarrayed with the corresponding antibody and count number in submicrometer area. To enhance reliability reproducibility approach, third-generation dendron was employed for surface treatment. specific between PSA detection (5A6) measured after cross-linking, mean unbinding 56 ± 2 pN. At 100 fM, there were 12 4.32 × 104 nm2, dependent upon concentration. A larger...