A5021499196- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Hematological disorders and diagnostics
- Lymphoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Immune Cell Function and Interaction
- Plant tissue culture and regeneration
- Blood disorders and treatments
- Hemoglobinopathies and Related Disorders
- Neutropenia and Cancer Infections
- Chronic Lymphocytic Leukemia Research
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- Eosinophilic Disorders and Syndromes
- Complement system in diseases
- Platelet Disorders and Treatments
- Plant Stress Responses and Tolerance
- Clinical Laboratory Practices and Quality Control
- Epigenetics and DNA Methylation
- Sepsis Diagnosis and Treatment
Catholic University of Korea
2016-2025
Seoul St. Mary's Hospital
2016-2025
Rural Development Administration
2023-2024
Korea Institute of Oriental Medicine
2019-2022
Korea Research Institute of Bioscience and Biotechnology
2021-2022
Gyeongsang National University
2011-2021
Korea University of Science and Technology
2021
Uijeongbu St. Mary's Hospital
2015-2020
Silicon Works (South Korea)
2016
Yonsei University
2001-2014
To evaluate diagnostic value of α-fetoprotein (AFP)-L3 and prothrombin induced by vitamin K absence-II (PIVKA-II) in hepatocellular carcinoma (HCC).One hundred sixty-eight patients during routine HCC surveillance were included this study. Of the 168 patients, 90 (53.6%) had including newly developed (n = 82) or recurrent after treatment 8). Sera obtained their first evaluation for development at time diagnosis before commencing treatment. was diagnosed histological examination, appropriate...
Abstract Human perinatal tissue is an abundant source of mesenchymal stromal cells(MSCs) and lacks the ethical concerns. Perinatal MSCs can be obtained from various tissues as like amnion, chorion, umbilical cord. Still, little known distinct nature each MSC type. In this study, we successfully isolated cultured amnion(AMSCs), chorion(CMSCs), cord(UC-MSCs). Proliferation potential was different among them, that AMSCs revealed lowest proliferation rate due to increased Annexin V...
Abstract Background The revised definition of sepsis is life‐threatening organ dysfunction caused by a dysregulated host response to infection (SEPSIS‐3). objective this study was evaluate procalcitonin (PCT) for the diagnosis and prognosis using SEPSIS‐3. Methods We enrolled 248 patients, who were admitted emergency department with suspected bacterial from June 2016 February 2017. Definite defined proven culture results, probable based on diagnostic modalities other than culture. sequential...
Pluripotent stem cell transplantation is a promising regenerative strategy for treating intractable diseases. However, securing human leukocyte antigen (HLA)-matched donor cells extremely difficult. The traditional approach generating such to establish homozygous pluripotent lines. Unfortunately, because of HLA diversity, this too time-consuming be practical use. engineering has been proposed recently as means evade graft-versus-host rejection in allotransplantation. This would advantageous...
Background: Many authors have reported good results with the use of vascularized fibular grafts to treat large osteonecrotic lesions femoral head. To our knowledge, there been no prospective case-controlled studies comparing effectiveness grafting that nonvascularized for prevention progression and collapse lesion. Methods: Nineteen patients (twenty-three hips) a lesion head (Stage IIC in ten hips, Stage IIIC two, IVC eleven, according classification system Steinberg et al.) underwent...
Excessive systemic inflammation following sepsis, trauma or burn could lead to multi-organ damage and death.Bone marrow stromal cells (BMSCs), commonly referred as mesenchymal stem (MSCs), has been studied in several immune-associated diseases human animal by modulating the inflammatory response.Adipose tissue derived (ATSCs), which can be obtained more easily, compared with BMSCs, emerged an attractive alternative MSCs source for cell therapy.We investigated therapeutic effects of ATSCs...
We identified principal genetic alterations in 97.1% (99/102) of patients with T-acute lymphoblastic leukemia (T-ALL) using integrative analyses, including massive parallel sequencing and multiplex ligation-dependent probe amplification (MLPA). A total 133 mutations were the following genes descending order: NOTCH1 (66.7%), FBXW7 (19.6%), PHF6 (15.7%), RUNX1 (12.7%), NRAS (10.8%), DNMT3A (9.8%). Copy number most frequently detected CDKN2B, CDKN2A, on 9p21.3 T-ALL (45.1%). Gene expression...
The object of this study was to evaluate biomarkers for diagnosis sepsis, hematologic parameters, and cytokine profiles use in the evaluation severity sepsis.We enrolled 127 consecutive patients with systemic inflammatory response syndrome (SIRS), 97 whom were diagnosed sepsis. following evaluated: procalcitonin (PCT); C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); white blood cell count, immature granulocyte (IG) count; multiplex cytokines, including interleukin (IL)1-β...
Human mesenchymal stromal cells (MSCs) have served as a major cellular resource for cell-based immunomodulatory and regenerative therapies. However, genomic instability may accumulate during ex vivo expansion of MSCs, thereby increasing the potential malignant transformation. Here, we performed whole genome sequencing two peripheral blood-derived MSC lines (MSC1 MSC2) at various passages (passage 1 [P1] to P9). The majority single-nucleotide variations (SNVs) occurred in later passages;...
To validate the clinical application of chromosomal microarray analysis (CMA) as a first-tier diagnostic test and to determine impact CMA results on patient management, we conducted multicenter prospective study in Korean patients diagnosed having developmental delay/intellectual disability (DD/ID), autism spectrum disorders (ASD), multiple congenital anomalies (MCA).We performed both G-banding cytogenetics tests 617 patients. whether directly influenced treatment recommendations, referring...
DNMT3A mutations occur in approximately 20% of AML cases and are associated with changes DNA methylation. CDKN2B plays an important role the regulation hematopoietic progenitor cells mutation is promoter We analyzed characteristics including their clinical significance influence on methylation expression.A total 142 adults, recently diagnosed de novo AML, were enrolled study. Mutations DNMT3A, CEBPA, NPM1 by bidirectional Sanger sequencing. evaluated expression using pyrosequencing...
Abstract Given limited studies on next-generation sequencing-based measurable residual disease (NGS-MRD) in acute myeloid leukemia (AML) patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT), we longitudinally collected samples before and allo-HSCT from two independent prospective cohorts ( n = 132) investigated the prognostic impact of amplicon-based NGS assessment. Persistent mutations were detected pre-HSCT (43%) 1 month HSCT (post-HSCT-1m, 20%). All persistent at...
Prunus africana is an endangered medicinal plant and hence new propagation methods are urgently required to increase its populations. Unfortunately, through seeds challenging due long flowering cycle recalcitrant seeds. We developed a protocol for micropropagation using nodal segment explants. A woody medium supplemented with vitamins, 15 g L −1 sucrose, 1.0 mg 6-benzylaminopurine (BAP) supported the optimum rate (100%) of axillary shoot initiation. Supplementation sucrose 1.5...
We evaluated the characteristics of bone marrow stromal cells (BMSCs) and hematopoietic (HCs) from patients myelodysplastic syndrome (MDS, n=21) acute myeloid leukemia (AML, n=58), compared results with control BMSCs derived healthy donors (n=8). The patient had lower proliferative activity than that controls due to increased senescence. This retarded proliferation induced failure obtain enough metaphase for karyotyping in (10%). Patient were genetically altered which was demonstrated by...
Filaggrin is essential for the development of skin barrier. Mutations in gene encoding filaggrin have been identified as major predisposing factors atopic disorders. Molecular analysis FLG this study showed nine null and one unclassified mutation 13 81 Korean patients with dermatitis (AD): five novel mutations (i.e. p.S1405*, c.5671_5672delinsTA, p.W1947*, p.G2025* p.E3070*); four reported c.3321delA, p.S1515*, p.S3296* p.K4022*); c.306delAAAGCACAG). These variants are nonsense, premature...
Monitoring minimal residual disease (MRD) provides important information during treatment of hematologic malignancies. Chimerism analysis also key after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent advances in next-generation sequencing (NGS) have enabled identification various mutations and quantification mutant allele burden. In this study, we developed a new analytic algorithm to monitor chimerism applicable NGS multi-gene panel use identify myelodysplastic...
Microbial contamination of platelet concentrates (PC) remains a persistent challenge in transfusion medicine, necessitating robust preventive measures prior to product release. Differentially expressed gene (DEG) analysis microbe inoculated PC offers promising approach identifying potential biomarkers for detection. Within PC, each S. aureus (ATCC 29213) and epidermidis 12228) was 103 CFU/mL concentration. Total RNA extracted from the samples at predetermined time points (0-, 1-, 3-, 6-hours...
Background: Aplastic anemia (AA) is a rare bone marrow failure syndrome characterized by notably short telomere length, which associated with treatment responses. In this study, we measured lengths in Korean pediatric AA patients using flow-fluorescence situ hybridization (Flow-FISH) and explored their shortening relation to disease characteristics, genetic conditions patient outcomes. Methods: We analyzed peripheral blood samples from 75 101 healthy controls. Telomere were Flow-FISH,...