Abstract Human perinatal tissue is an abundant source of mesenchymal stromal cells(MSCs) and lacks the ethical concerns. Perinatal MSCs can be obtained from various tissues as like amnion, chorion, umbilical cord. Still, little known distinct nature each MSC type. In this study, we successfully isolated cultured amnion(AMSCs), chorion(CMSCs), cord(UC-MSCs). Proliferation potential was different among them, that AMSCs revealed lowest proliferation rate due to increased Annexin V...

The casein kinase 2 (CK2) protein is a pro-survival and therapeutic target in treatment of various human cancers. CK2 overexpression has been demonstrated hematological malignancies, including chronic lymphocytic leukemia, myeloid acute lymphoblastic multiple myeloma. CX-4945, also known as Silmitasertib, an orally administered, highly specific, ATP-competitive inhibitor CK2. CX-4945 induces cytotoxicity apoptosis currently being evaluated clinical trials for many cancer types. In the past...

BackgroundThe epithelial-to-mesenchymal transition (EMT) is a major phenotype of cancer metastasis and invasion. As druggable target, the inhibition protein kinase CK2 (formally named to casein 2) has been suggested as promising therapeutic strategy treat EMT-controlled metastasis. This study aimed evaluate effect inhibitor CX-4945 on processes migration invasion during EMT in A549 human lung adenocarcinoma cells. Materials MethodsThe TGF-β1-induced was evaluated cells treated with TGF-β1 (5...

Abstract Background The nematode infraorder Tylenchomorpha (Class Chromadorea) includes plant parasites that are of agricultural and economic importance, as well insect-associates fungal feeding species. Among tylenchomorph parasites, members the superfamily Tylenchoidea, such root-knot nematodes, have great impact on agriculture. Of five superfamilies within Tylenchomorpha, one (Aphelenchoidea) mainly fungal-feeding species, but also some damaging pathogens, including certain...

This study investigated special education teachers’ perceptions of artificial intelligence digital textbooks(AIDT) and analyzed differences based on various background variables. Data were collected through an online survey 199 teachers working in schools. Descriptive statistics one-way ANOVA used for data analysis. The results revealed that, first, showed interest AIDT generally exhibited a more-than-average positive attitude toward its implementation; however, their understanding...

Transforming growth factor (TGF)-β triggers the epithelial-to-mesenchymal transition (EMT) of cancer cells via well-orchestrated crosstalk between Smad and non-Smad signaling pathways, including Wnt/β-catenin. Since EMT-induced motility invasion play a critical role in metastasis, EMT-related molecules are emerging as novel targets anti-cancer therapies. Traf2- Nck-interacting kinase (TNIK) has recently been considered first-in-class target molecule to regulate Wnt pathway, but pharmacologic...

Filaggrin is essential for the development of skin barrier. Mutations in gene encoding filaggrin have been identified as major predisposing factors atopic disorders. Molecular analysis FLG this study showed nine null and one unclassified mutation 13 81 Korean patients with dermatitis (AD): five novel mutations (i.e. p.S1405*, c.5671_5672delinsTA, p.W1947*, p.G2025* p.E3070*); four reported c.3321delA, p.S1515*, p.S3296* p.K4022*); c.306delAAAGCACAG). These variants are nonsense, premature...

Edelweiss (Leontopodium Alpinum) in the family Asteraceae is a wildflower that grows rocky limestone places. Here, we investigated efficacy of edelweiss callus culture extract Alpinum extract; LACCE) using multiple assays from vitro to vivo as well transcriptome profiling. Several assay results showed strong antioxidant activity LACCE response UVB treatment. Moreover, suppressed inflammation and wrinkling; however, moisturizing was increased by LACCE. The clinical test demonstrated constant...

This study focused on the characterization of mesenchymal stromal cells (MSCs) from chorion human full term placenta 15 donors. Chorionic MSCs revealed homologous fibroblast-like morphology and expressed CD73, CD29, CD105, CD90. The hematopoietic stem cell markers including HLA DR, CD11b, CD34, CD79a, CD45 were not expressed. growth kinetics their serial passage was steady at later passages (passage 10). multilineage capability chorionic demonstrated by successful adipogenic, osteogenic...

We report anti-cancer activity of ZnO thin-film-coated chips by sustained release zinc ions. were fabricated precisely tuning thickness using atomic layer deposition, and their potential to ions relative the number deposition cycles was evaluated. exhibited selective cytotoxicity in human B lymphocyte Raji cells while having no effect on peripheral blood mononuclear cells. Of importance, half-maximal inhibitory concentration chip viability 121.5 cycles, which comparable 65.7 nM daunorubicin,...

The undifferentiated spermatogonia of adult mouse testes are composed both true stem cells and committed progenitors. It is unclear what normally prevents these germ from manifesting multipotency. critical elements the spermatogonial cell niche, while poorly understood, thought to be Sertoli with several other somatic types in close proximity. We recently discovered a novel orphan G-protein coupled receptor (GPR125) that restricted within testis. GPR125 expression was maintained when...

Within the nematode class C hromadorea, suborder T ylenchina is an ecologically and morphologically diverse assemblage of nematodes that includes free‐living microbivores, fungivores various types plant parasites. A recent classification system based largely on SSU r DNA phylogenetic trees classified to include four infraorders: Panagrolaimomorpha, ephalobomorpha, ylenchomorpha D rilonematomorpha, relationships among species these infraorders have not always been robustly supported. In this...

Damask roses (Rosa x damascena) are widely used in cosmetics and pharmaceutics. Here, we established an vitro suspension cell culture for calli derived from damask rose petals. We analyzed transcriptomes obtained at two different time points by RNA sequencing to reveal transcriptional changes during culture. Of the 580 coding RNAs (1.3%) highly expressed cells, 68 encoded wall-associated proteins. However, most chloroplasts mitochondria not expressed. Many involved translation, catalyzing...

Abstract A series of flavonoids isolated from Scutellariae radix were evaluated for their effects on cytochrome P-450 (CYP) activities in human liver microsomes. All did not substantially inhibit pentoxyresorufin O -deethylation (CYP2B1), mephenytoin 4-hydroxylation (CYP2C19), dextromethorphan -demethylation (CYP2D6), and chlorzoxazone 6-hydroxylation (CYP2E1) (IC50: >50 µ M ). Baicalein 2',5,6',7-tetrahydroxyflavone inhibited hepatic testosterone 6 g -hydroxylation (CYP3A4) activity with a...

Drug repositioning can identify new therapeutic applications for existing drugs, thus mitigating high R&D costs. The Protein kinase 2 (CK2) inhibitor CX-4945 regulates human cancer cell survival and angiogenesis. Here we found that significantly inhibited the RANKL-induced osteoclast differentiation, but enhanced BMP2-induced osteoblast differentiation in a culture model. activation of TRAP NFATc1 expression accompanied with suppression Akt phosphorylation, contrast, it BMP2-mediated ALP...

Multiple myeloma is a fetal form of plasma cell malignancy characterized by abnormal clonal proliferation cells. Especially, the canonical Wnt signaling pathway mediated β-catenin activated in multiple cells, stimulating their proliferation. Here, we investigated relationship between interleukin-6-induced cells and Traf2- Nck-interacting kinase (TNIK) expression signaling. Interleukin-6 increased TNIK mRNA protein expression. In addition, examined effect on inhibitor KY-05009 receptor...