A5043910009- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Multiple Myeloma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Erythrocyte Function and Pathophysiology
- Hematological disorders and diagnostics
- Blood disorders and treatments
- Eosinophilic Disorders and Syndromes
- Bone and Joint Diseases
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- Congenital heart defects research
- RNA modifications and cancer
- Immunodeficiency and Autoimmune Disorders
- Blood groups and transfusion
- Immune Cell Function and Interaction
- Neutropenia and Cancer Infections
- Kruppel-like factors research
Catholic University of Korea
2016-2025
Seoul St. Mary's Hospital
2016-2025
Korean Red Cross
2025
Ewha Womans University Medical Center
2003-2024
Uijeongbu St. Mary's Hospital
2006-2024
Samsung Medical Center
2016-2023
Soonchunhyang University
2023
Bucheon St. Mary's Hospital
2013-2023
Weatherford College
2021
Soonchunhyang University Hospital
2013-2020
Abstract DEAD box helicase 41 (DDX41) mutations are the most prevalent predisposition to familial myelodysplastic syndrome (MDS). However, precise roles of these variants in pathogenesis MDS have yet be elucidated. Here, we discovered a novel mechanism by which DDX41 contributes R-loop-induced DNA damage responses (DDR) cooperation with m6A-METTL complex (MAC) and YTHDC1 using knockout (KO) knock-in (KI, R525H, Y259C) cell lines as well primary samples from patients. Compared wild type (WT),...
When large engineered tissue structures are used to achieve regeneration, formation of vasculature is an essential process. We report a technique that combines 3D printing with spatial and temporal control dual growth factors prevascularize bone tissue. Human dental pulp stem cells (DPSCs) have both osteogenic vasculogenic potential were printed morphogenetic protein-2 (BMP-2) in the peripheral zone construct, vascular endothelial factor (VEGF) central zone, which hypoxic area forms. The...
Abstract Acute myelogenous leukemia (AML) is a heterogeneous disorder characterized by clonal proliferation of stem cell-like blasts in bone marrow (BM); however, their unique cellular interaction within the BM microenvironment and its functional significance remain unclear. Here, we assessed AML patients demonstrate that cells induce change transcriptional programming normal mesenchymal stromal (MSC). The modified leukemic niche alters expressions cross-talk molecules (i.e., CXCL12 JAG1)...
To evaluate diagnostic value of α-fetoprotein (AFP)-L3 and prothrombin induced by vitamin K absence-II (PIVKA-II) in hepatocellular carcinoma (HCC).One hundred sixty-eight patients during routine HCC surveillance were included this study. Of the 168 patients, 90 (53.6%) had including newly developed (n = 82) or recurrent after treatment 8). Sera obtained their first evaluation for development at time diagnosis before commencing treatment. was diagnosed histological examination, appropriate...
Abstract Human perinatal tissue is an abundant source of mesenchymal stromal cells(MSCs) and lacks the ethical concerns. Perinatal MSCs can be obtained from various tissues as like amnion, chorion, umbilical cord. Still, little known distinct nature each MSC type. In this study, we successfully isolated cultured amnion(AMSCs), chorion(CMSCs), cord(UC-MSCs). Proliferation potential was different among them, that AMSCs revealed lowest proliferation rate due to increased Annexin V...
Abstract Background The revised definition of sepsis is life‐threatening organ dysfunction caused by a dysregulated host response to infection (SEPSIS‐3). objective this study was evaluate procalcitonin (PCT) for the diagnosis and prognosis using SEPSIS‐3. Methods We enrolled 248 patients, who were admitted emergency department with suspected bacterial from June 2016 February 2017. Definite defined proven culture results, probable based on diagnostic modalities other than culture. sequential...
Pluripotent stem cell transplantation is a promising regenerative strategy for treating intractable diseases. However, securing human leukocyte antigen (HLA)-matched donor cells extremely difficult. The traditional approach generating such to establish homozygous pluripotent lines. Unfortunately, because of HLA diversity, this too time-consuming be practical use. engineering has been proposed recently as means evade graft-versus-host rejection in allotransplantation. This would advantageous...
Mesenchymal stromal cells derived from induced pluripotent stem (iMSCs) have been proposed as alternative sources of primary MSCs with various advantages for cell therapeutic trials. However, precise evaluation the differences between iMSCs and is lacking due to individual variations in donor cells, which obscure direct comparisons two. In this study, we generated donor-matched bone marrow-derived directly compared their cell-autonomous paracrine effects. We found that transition accompanied...
The emergence and evolution of SARS-CoV-2 variants, such as Delta Omicron, pose significant challenges to pandemic management. This study evaluated the effectiveness reverse-transcription polymerase chain reaction (RT-PCR) whole-genome sequencing (WGS) in detecting characterizing variants using 624 samples collected South Korea from mid-2021 mid-2022. Two RT-PCR genotyping assays demonstrated a high concordance rate (90.4%) identifying variant during its dominance. In contrast, WGS revealed...
Recently, resistance to extended-spectrum cephalosporins due acquired beta-lactamases has been reported in Pseudomonas aeruginosa. The aim of this study was investigate the prevalence Ambler class A and D their derivatives antimicrobial susceptibilities P. aeruginosa isolated from various parts Korea.A total 252 consecutive, non-duplicate isolates were studied for presence or beta-lactamase. Antibiotic susceptibility tests PCR amplification genes encoding (bla(PSE-1), bla(PER-1), bla(VEB-1),...
The aim of this study was to describe the mutational characteristics in Korean hereditary spherocytosis (HS) patients. Relevant literatures including genetically confirmed cases with well-documented clinical summaries and relevant information were also reviewed investigate gene- or domain-specific laboratory association. Twenty-five HS patients carried one heterozygous mutation ANK1 (n = 13) SPTB 12) but not SPTA1, SLC4A1, EPB42. Deleterious mutations frameshift, nonsense, splice site...
To characterize the incidence, clinical features and genetics of ETV6-ABL1 leukemias, representing targetable kinase-activating lesions, we analyzed 44 new published cases ETV6-ABL1-positive hematologic malignancies [22 acute lymphoblastic leukemia (13 children, 9 adults) 22 myeloid (18 myeloproliferative neoplasms, 4 leukemias)]. The presence fusion was ascertained by cytogenetics, fluorescence in-situ hybridization, reverse transcriptase-polymerase chain reaction RNA sequencing. Genomic...
We identified principal genetic alterations in 97.1% (99/102) of patients with T-acute lymphoblastic leukemia (T-ALL) using integrative analyses, including massive parallel sequencing and multiplex ligation-dependent probe amplification (MLPA). A total 133 mutations were the following genes descending order: NOTCH1 (66.7%), FBXW7 (19.6%), PHF6 (15.7%), RUNX1 (12.7%), NRAS (10.8%), DNMT3A (9.8%). Copy number most frequently detected CDKN2B, CDKN2A, on 9p21.3 T-ALL (45.1%). Gene expression...
The object of this study was to evaluate biomarkers for diagnosis sepsis, hematologic parameters, and cytokine profiles use in the evaluation severity sepsis.We enrolled 127 consecutive patients with systemic inflammatory response syndrome (SIRS), 97 whom were diagnosed sepsis. following evaluated: procalcitonin (PCT); C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); white blood cell count, immature granulocyte (IG) count; multiplex cytokines, including interleukin (IL)1-β...
Human mesenchymal stromal cells (MSCs) have served as a major cellular resource for cell-based immunomodulatory and regenerative therapies. However, genomic instability may accumulate during ex vivo expansion of MSCs, thereby increasing the potential malignant transformation. Here, we performed whole genome sequencing two peripheral blood-derived MSC lines (MSC1 MSC2) at various passages (passage 1 [P1] to P9). The majority single-nucleotide variations (SNVs) occurred in later passages;...
Mutation of the fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD), which is one most frequent genetic alterations, strongly contributes to an increased risk treatment failure and poor prognosis. In this study, we established quantitative fragment analysis FLT3-ITD simultaneously measuring mutant allele burden length, verified analytical performance evaluated clinical significance in adult acute myeloid leukemia (AML) patients. was detected 73 363 AML patients (20.1%) high...
To validate the clinical application of chromosomal microarray analysis (CMA) as a first-tier diagnostic test and to determine impact CMA results on patient management, we conducted multicenter prospective study in Korean patients diagnosed having developmental delay/intellectual disability (DD/ID), autism spectrum disorders (ASD), multiple congenital anomalies (MCA).We performed both G-banding cytogenetics tests 617 patients. whether directly influenced treatment recommendations, referring...
DNMT3A mutations occur in approximately 20% of AML cases and are associated with changes DNA methylation. CDKN2B plays an important role the regulation hematopoietic progenitor cells mutation is promoter We analyzed characteristics including their clinical significance influence on methylation expression.A total 142 adults, recently diagnosed de novo AML, were enrolled study. Mutations DNMT3A, CEBPA, NPM1 by bidirectional Sanger sequencing. evaluated expression using pyrosequencing...
Abstract Given limited studies on next-generation sequencing-based measurable residual disease (NGS-MRD) in acute myeloid leukemia (AML) patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT), we longitudinally collected samples before and allo-HSCT from two independent prospective cohorts ( n = 132) investigated the prognostic impact of amplicon-based NGS assessment. Persistent mutations were detected pre-HSCT (43%) 1 month HSCT (post-HSCT-1m, 20%). All persistent at...
Aquaporins (AQPs) have previously been associated with increased expression in solid tumors. However, its hematologic malignancies including CML has not described yet. Here, we report the of AQP5 cells by RT-PCR and immunohistochemistry. While normal bone marrow biopsy samples (n = 5) showed no AQP5, 32% patient 41) demonstrated expression. In addition, level emergence imatinib mesylate resistance paired (p 0.047). We found that overexpression K562 resulted cell proliferation. small...
Abstract With contrasting observations on the effects of β-catenin hematopoietic stem cells (HSCs), precise role Wnt/β-catenin signals HSC regulation remains unclear. Here, we show a distinct mode signal that can regulate HSCs in stroma-dependent manner. Stabilization bone marrow stromal promoted maintenance and self-renewal contact-dependent manner, whereas direct stabilization caused loss HSCs. Interestingly, canonical Wnt receptors accumulation were predominantly enriched rather than...
Recurrent somatic mutations in splicing machinery components, including SF3B1, U2AF1 and SRSF2 genes have recently been reported myelodysplastic syndromes (MDS). Such a recurrent nature strongly suggests that these play important roles tumor development. To see whether occur other human tumors besides MDS, we analyzed the hotspot mutation regions of 2,345 tissues from various origins (61 616 hematologic tumors, 1,421 epithelial 247 non-epithelial stromal tumors) by single-strand conformation...