A5019156869- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Advanced Glycation End Products research
- Pancreatic function and diabetes
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Adipose Tissue and Metabolism
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Birth, Development, and Health
- PARP inhibition in cancer therapy
- Endometrial and Cervical Cancer Treatments
- Genetics and Neurodevelopmental Disorders
- Effects of Radiation Exposure
- Metabolism, Diabetes, and Cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Angiogenesis and VEGF in Cancer
- Autophagy in Disease and Therapy
- Melanoma and MAPK Pathways
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Kruppel-like factors research
Ulsan National Institute of Science and Technology
2021-2024
Korea University of Science and Technology
2021
First Affiliated Hospital of Zhengzhou University
2021
Pusan National University
2011-2019
Chosun University
2012
Ajou University
2012
Abstract DEAD box helicase 41 (DDX41) mutations are the most prevalent predisposition to familial myelodysplastic syndrome (MDS). However, precise roles of these variants in pathogenesis MDS have yet be elucidated. Here, we discovered a novel mechanism by which DDX41 contributes R-loop-induced DNA damage responses (DDR) cooperation with m6A-METTL complex (MAC) and YTHDC1 using knockout (KO) knock-in (KI, R525H, Y259C) cell lines as well primary samples from patients. Compared wild type (WT),...
Wnt signaling plays a critical role in embryonic development, and its deregulation is closely linked to the occurrence of number malignant tumors, including breast colon cancer. The pathway also induces Snail-dependent epithelial-to-mesenchymal transition (EMT), which responsible for tumor invasion metastasis. In this study, we show that suppresses mitochondrial respiration cytochrome C oxidase (COX) activity by inhibiting expression 3 COX subunits, namely, COXVIc, COXVIIa, COXVIIc. We found...
// Su Yeon Lee 1 , Hyun Min Jeon Kyung Ju Eui Kyong Jeong Cho Hee Kim 1, * Hye Gyeong Park 2 Song Iy Han 3 Ho Sung Kang Department of Molecular Biology, College Natural Sciences, Pusan 609-735, Korea Nanobiotechnology Center, National University, The Division Medical Health Science, Chosun Gwangju 501-759, Present address: DNA Identification Forensic Service, Seoul 158-707, Correspondence to: Kang, e-mail: hspkang@pusan.ac.kr Keywords: Dlx-2, GLS1, Snail, epithelial-mesenchymal transition,...
Reactive oxygen species (ROS) are important cellular second messengers involved in various aspects of cell signaling. ROS elevated multiple types cancer cells, and this elevation is known to be pathological processes cancer. Although high levels exert cytotoxic effects on low stimulate proliferation survival by inducing several pro‑survival signaling pathways. In addition, have been shown induce epithelial‑mesenchymal transition (EMT), which essential for the initiation metastasis. However,...
Epithelial-mesenchymal transition (EMT) and oncogenic metabolism (including glycolytic switch) are important for tumor development progression. Here, we show that Dlx-2, one of distal-less (Dlx) homeobox genes, induces EMT switch by activation Snail. In addition, it was induced TGF-β Wnt regulates TGF-β- Wnt-induced activating We also found TGF-β/Wnt suppressed cytochrome c oxidase (COX), the terminal enzyme mitochondrial respiratory chain, in a Dlx-2/Snail-dependent manner. appeared to...
Replication protein A (RPA), a eukaryotic single-stranded DNA (ssDNA) binding protein, dynamically interacts with ssDNA in different modes and plays essential roles metabolism such as replication, repair, recombination. RPA accumulation on due to replication stress triggers the damage response (DDR) by activating ataxia telangiectasia RAD3-related (ATR) kinase, which phosphorylates itself downstream DDR factors, including RPA. We recently reported that N-methyl-D-aspartate receptor...
Cancer cells frequently fail to respond chemotherapy due acquisition of chemoresistance. Tumour are prone die by necrosis when they metabolically stressed hypoxic and glucose depletion (OGD) insufficient vascularization, a common feature solid tumours. indicates poor prognosis emergence drug resistance in cancer patients; however, its molecular mechanism remains unclear. In this study, we used multicellular tumour spheroids (MTS) as an vitro model investigate the mechanisms underlying...
Abstract Background In contrast to tumor-suppressive apoptosis and autophagic cell death, necrosis promotes tumor progression by releasing the pro-inflammatory tumor-promoting cytokine high mobility group box 1 (HMGB1), its presence in patients is associated with poor prognosis. Thus, has important clinical implications development; however, molecular mechanism remains poorly understood. Results present study, we show that Distal-less 2 (Dlx-2), a homeobox gene of Dlx family involved...
Necrosis, a type of cell death accompanied by the rupture plasma membrane, promotes tumor progression and aggressiveness releasing pro-inflammatory angiogenic cytokine high mobility group box 1. It is commonly found in core region solid tumors due to hypoxia glucose depletion (GD) resulting from insufficient vascularization. Thus, metabolic stress-induced necrosis has important clinical implications for development; however, its regulatory mechanisms have been poorly investigated.Here, we...
Invasion and metastasis of cervical cancer are the main factors affecting prognosis patients with squamous cell carcinoma (CESC). Therefore, it is vital importance to find novel biomarkers that associated CESC invasion metastasis, which will aid in amelioration individualized therapeutic methods for advanced patients.The gene expression profiles 10 metastatic 116 non-metastatic samples were downloaded from The Cancer Genome Atlas (TCGA), where differentially expressed genes (DEGs) defined....
Proper activation of DNA repair pathways in response to replication stress is critical for maintaining genomic integrity. Due the complex nature fork (RF), problems at RF require multiple proteins, some which remain unidentified, resolution. In this study, we identified N-methyl-D-aspartate receptor synaptonuclear signaling and neuronal migration factor (NSMF) as a key that important ataxia telangiectasia Rad3-related protein (ATR) activation. NSMF localizes rapidly stalled RFs acts scaffold...
Epithelial-to-mesenchymal transition (EMT)는 embryogenesis에서 중요한 역할을 하며 tumor metastasis, invasion에도 관여함으로써 progression 및 aggressiveness에 기여한다. EMT는 EMT hallmark인 epithelial E-cadherin의 발현 감소와 mesenchymal-like cell morphology를 획득함으로써 polarity를 잃어버리는 특징을 가지고 있다. <TEX>$O_2{^-}$</TEX>, <TEX>$H_2O_2$</TEX>, <TEX>$OH^-$</TEX>와 같은 활성산소가 EMT를 유도하는 것으로 알려져 Snail이 발현을 억제함으로써 ROS에 의한 EMT에 관여하는 있으나, 그 기작은 완전히 밝혀져 있지 않다. 본 연구에서는, noninvasive breast line인 MCF-7 세포에 Egr-1을 과발현시킨 후 영향을 조사하였다. Egr-1이 과발현되면,...
<div>Abstract<p>Wnt signaling plays a critical role in embryonic development, and its deregulation is closely linked to the occurrence of number malignant tumors, including breast colon cancer. The pathway also induces Snail-dependent epithelial-to-mesenchymal transition (EMT), which responsible for tumor invasion metastasis. In this study, we show that Wnt suppresses mitochondrial respiration cytochrome C oxidase (COX) activity by inhibiting expression 3 COX subunits, namely,...
<p>PDF file - 3.8MB, Supplemental Figures S1-S7 include the Induction of EMT in MCF-7 cells (Figure S1), effects Wnt on mitochondrial mass S2), Ponceau S staining corresponding to Figure 4A S3), ChIP assays using Wnt3a-treated or Snail-transfected S4), repression and glycolytic switch by PC shRNA S5), E-cadherin Snail expression COXVIc, COXVIIa, COXVIIc, S6), a schematic representation showing new function Wnt/Snail S7)</p>
<p>PDF file - 152K, File includes Western blotting, RT-PCR, real-time PCR, and nuclear fractionation</p>
<p>PDF file - 144K</p>
<p>PDF file - 3.8MB, Supplemental Figures S1-S7 include the Induction of EMT in MCF-7 cells (Figure S1), effects Wnt on mitochondrial mass S2), Ponceau S staining corresponding to Figure 4A S3), ChIP assays using Wnt3a-treated or Snail-transfected S4), repression and glycolytic switch by PC shRNA S5), E-cadherin Snail expression COXVIc, COXVIIa, COXVIIc, S6), a schematic representation showing new function Wnt/Snail S7)</p>