A5023618406- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Arsenic contamination and mitigation
- Microbial infections and disease research
- Advanced biosensing and bioanalysis techniques
- Chromium effects and bioremediation
- Identification and Quantification in Food
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Machine Learning in Bioinformatics
- RNA modifications and cancer
- Biosensors and Analytical Detection
- Iron Metabolism and Disorders
- Protist diversity and phylogeny
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
Pusan National University
2015-2025
Hallym University Sacred Heart Hospital
2023
Wooridul Hospital
2014
Samsung Medical Center
2005-2012
Sungkyunkwan University
2005-2012
Samsung (South Korea)
2012
Theragen
2011
National Institute of Animal Science
2008
Arizona State University
2007
Washington University in St. Louis
2002-2007
Skin tissue is susceptible to oxidative stress-induced senescence provoked by ultraviolet (UV) exposure in our daily lives, resulting photoaging. Herein, we explore whether N-benzyl-N-methyldecan-1-amine (BMDA) derived from garlic ameliorates UVB-induced To address this issue, HaCaT keratinocytes were exposed UVB irradiation under BMDA treatment. The presence of substantially reduced ROS levels a dose-dependent manner. administration counteracted the β-galactosidase assay. Treatment with...
ABSTRACT Attenuated Salmonella enterica serovar Typhimurium expressing recombinant antigens from other pathogens elicits primarily a Th1-type dominant immune response to both and antigens. The immunogenicity appropriate subcellular location of the antigen in vaccine strain may contribute augmenting responses by facilitating adequate exposure antigen-presenting cells for processing. To allow secretion gram-negative bacteria overexpression antigen, DNA fragment encoding highly antigenic...
Reactive oxygen species (ROS) are important cellular second messengers involved in various aspects of cell signaling. ROS elevated multiple types cancer cells, and this elevation is known to be pathological processes cancer. Although high levels exert cytotoxic effects on low stimulate proliferation survival by inducing several pro‑survival signaling pathways. In addition, have been shown induce epithelial‑mesenchymal transition (EMT), which essential for the initiation metastasis. However,...
Tetrandrine, isolated from the root of Stephania tetrandra, has been used in Chinese medicine for treatment silicosis and arthritis, it also anti-tumor/growth activities. However, signaling pathways tetrandrine-induced growth arrest apoptosis cancer cells remain unclear. We investigated molecular mechanisms human lung carcinoma cells. Upon with tetrandrine, a time-dependent inhibition cell was observed developed many hallmark features apoptosis. Flow cytometry analysis confirmed that...
The subcellular location of a recombinant antigen in attenuated Salmonella vaccines may influence immunogenicity dependent on exposure the to cells involved systemic immune responses. It has been shown that vaccine secreting Streptococcus pneumoniae PspA (rPspA) specified by pYA3494 induced protective anti-rPspA-specific responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A plasmid pYA3496 specifying His(6)-tagged rPspA (His(6)-rPspA) protein (no apparent signal sequence) caused...
Preclinical and clinical studies have shown that the application of CD105+ mesenchymal stem cells (MSCs) is feasible may lead to recovery after stroke. In addition, circulating microparticles are reportedly functional in various disease conditions. We tested levels patients with acute ischemic The expression CD105 (a surface marker MSCs) CXCR4 CXC chemokine receptor for MSC homing) on was evaluated by flow cytometry samples from 111 50 healthy subjects. percentage apoptotic determined based...
Oligonucleotide-based aptamers, which have a three-dimensional structure with single-stranded fragment, feature various characteristics respect to size, toxicity, and permeability. Accordingly, aptamers are advantageous in terms of diagnosis treatment materials that can be produced through relatively simple experiments. Systematic evolution ligands by exponential enrichment (SELEX) is one the most widely used experimental methods for generating aptamers; however, it highly expensive...
A challenge in strain construction is that unmarked deletion and nucleotide substitution alleles generally do not confer selectable phenotypes. We describe here a rapid efficient strategy for transferring such via generalized transduction. The desired allele first constructed introduced into the chromosome by conventional allelic-exchange methods. suicide vector containing same then integrated mutant chromosome, generating tandem duplication homozygous allele. resulting used as donor...
Overexpression of HIF-1α, a transcription factor responsive to hypoxia, is frequently observed in malignant tumors, which sometimes show resistance chemotherapy and radiation therapy. Consequently, decrease HIF-1α through virotherapy offers logical strategy for the treatment aggressive tumors. In this study, we found that infection with oncolytic H-1 parvovirus decreased protein levels pancreatic cancer cells under CoCl2 or hypoxia. The virus-induced was regulated by proteasome-mediated...
Given the intricate etiology and pathogenesis of atopic dermatitis (AD), complete cure AD remains challenging. This study aimed to investigate if topically applying N-benzyl-N-methyldecan-1-amine (BMDA), derived from garlic, its derivative [decyl-(4-methoxy-benzyl)-methyl-1-amine] (DMMA) could effectively alleviate AD-like skin lesions in 2,4-dinitrochlorobenzene (DNCB)-treated mice. Administering these compounds irritated DNCB-treated mice significantly reduced swelling, rash, excoriation...
ABSTRACT Genetic and biochemical studies have established that Fur iron mediate repression of Bordetella alcaligin siderophore system ( alc ) genes under iron-replete nutritional growth conditions. In this study, transcriptional analyses using chromosomal alc-lacZ operon fusions determined maximal gene activity starvation stress conditions is dependent on the presence siderophore. Mutational analysis genetic complementation confirmed alcaligin-responsive activation AlcR, a Fur-regulated...
ABSTRACT A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The displayed a growth defect iron-restricted medium containing ferric alcaligin as the sole source. In addition to apparent inability acquire from siderophore, failed produce well two known iron-regulated proteins, one of which is AlcC biosynthesis protein. 1.6-kb Kpn I- Pst I pertussis DNA fragment mapping downstream genes alcABC restored both...
There is no doubt that the therapeutic efficacy of mesenchymal stem cells (MSCs) needs improvement. SDF-1 (chemokine for MSC homing) and its receptor CXCR4 play a critical role in migration MSCs ischemia. We investigated effects application transfected to overexpress using an adenoviral construct rat stroke model. Both flow cytometry Western blot analysis indicated level expression was low naive hMSCs but consistently high CXCR4-hMSCs. In vivo test transwell system showed degree increased...
Sodium salicylate is known to induce apoptosis in a variety of cancer cells. However, the molecular mechanism for salicylate-induced yet unclear. Here we show that HCT116 colon carcinoma cells, 10 mM sodium induces caspase-3 activation and degradation its substrates, poly(ADP-ribose) polymerase (PARP), beta-catenin, retinoblastoma (Rb). In contrast, did not exert any significant effects on expression Fas L implicated extrinsic apoptotic pathway levels Bcl-2 family proteins, Bcl-2, Bcl-xsl,...
Animal behavioral responses have been recently established as a suitable tool for detecting contaminants in the environment risk assessment situ. In this study, we observed movement behavior of zebrafish (Danio rerio) before and after infection with Edwardsiella tarda CK41 3 days until death. Infection status was confirmed through PCR colonization assay time progressed lesion development tails also examined. Movement behaviors response to bacterial were patterned by self-organizing map (SOM)...