A5042523814- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood disorders and treatments
- Hematological disorders and diagnostics
- Erythrocyte Function and Pathophysiology
- Hematopoietic Stem Cell Transplantation
- Epigenetics and DNA Methylation
- Platelet Disorders and Treatments
- Genetic factors in colorectal cancer
- Neutropenia and Cancer Infections
- Immune Cell Function and Interaction
- Congenital heart defects research
- SARS-CoV-2 and COVID-19 Research
- Chronic Lymphocytic Leukemia Research
- Cardiac electrophysiology and arrhythmias
- Antibiotic Resistance in Bacteria
- Cardiovascular Function and Risk Factors
- Blood groups and transfusion
- Renal Transplantation Outcomes and Treatments
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 detection and testing
- Cardiomyopathy and Myosin Studies
- Biochemical and biochemical processes
- Enterobacteriaceae and Cronobacter Research
Seoul St. Mary's Hospital
2018-2025
Catholic University of Korea
2018-2025
Korea Advanced Institute of Science and Technology
2012
Abstract Given limited studies on next-generation sequencing-based measurable residual disease (NGS-MRD) in acute myeloid leukemia (AML) patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT), we longitudinally collected samples before and allo-HSCT from two independent prospective cohorts ( n = 132) investigated the prognostic impact of amplicon-based NGS assessment. Persistent mutations were detected pre-HSCT (43%) 1 month HSCT (post-HSCT-1m, 20%). All persistent at...
Abstract The prevention and management of cancer therapy‐related cardiac dysfunction (CTRCD) have become increasingly important. Recent studies revealed the crucial role genetics in determining susceptibility to development CTRCD. We present a case 65‐year‐old woman with breast who developed recurrent CTRCD following low‐dose chemotherapy, despite lacking conventional cardiovascular risk factors. Her medical history included anthracycline‐associated cardiomyopathy, her condition deteriorated...
Microbial contamination of platelet concentrates (PC) remains a persistent challenge in transfusion medicine, necessitating robust preventive measures prior to product release. Differentially expressed gene (DEG) analysis microbe inoculated PC offers promising approach identifying potential biomarkers for detection. Within PC, each S. aureus (ATCC 29213) and epidermidis 12228) was 103 CFU/mL concentration. Total RNA extracted from the samples at predetermined time points (0-, 1-, 3-, 6-hours...
Although natural killer (NK) cell function is a hallmark of hemophagocytic lymphohistiocytosis (HLH), there no standard method or data on its diagnostic value in adults. Thus, we performed single-center retrospective study 119 adult patients with suspected HLH. NK was determined using both flowcytometry-based NK-cytotoxicity test (NK-cytotoxicity) and activity for interferon-gamma (NKA-IFNγ). phenotype serum cytokine levels were also tested. Fifty (42.0%) HLH showed significantly reduced...
Chronic lymphocytic leukemia (CLL) is the most common adult in Western countries. However, CLL relatively rare Asia; its genetic features are rarely studied. Here, we aimed to genetically characterize Korean patients and elucidate clinical associations based on data obtained from 113 at a single institute. We used next-generation sequencing explore multi-gene mutational immunoglobulin heavy chain variable gene clonality with somatic hypermutation (SHM). MYD88 (28.3%), including L265P (11.5%)...
The Luminex-based single antigen bead (SAB) assay is widely used to detect HLA antibody in transplant recipients. However, one limitation of the SAB prozone effect, which occurs mostly as a result complement interference. We investigated efficacy EDTA treatment for overcoming effect and predicting C1q binding antibody. subjected 27 non-treated (naïve) EDTA-treated serum samples from highly sensitized patients IgG-SAB assays, we confirmed 53% 31% class I II tests, respectively, after...
Background: Previous studies have reported that genes highly expressed in leukemic stem cells (LSC) may dictate the survival probability of patients and expression-based cellular deconvolution be informative forecasting prognosis. However, whether prognosis acute myeloid leukemia (AML) can predicted using gene expression deconvoluted abundances is debatable. Methods: Nine different cell-type a training set composed AML samples 422 patients, were used to build model for predicting by least...
Abstract A domino olefin metathesis reaction of 1‐alkyl‐3‐(allyloxy)‐7‐oxabicyclo[2.2.1]hept‐5‐enes 7 produced the fused bis(oxacyclic) structure for dysiherbaine and neodysiherbaine (i.e., 6b ) with complete control relative stereochemistry at quaternary carbon center ring junction core skeleton. formal total synthesis was achieved from .
Pelvic organ prolapse (POP) is a benign gynecological disease in which the pelvic descends into vagina and causes voiding, defecatory dysfunction, mainly occurs older women. This study aimed to investigate vaginal microbiome of POP associated changes after anatomical restorative pessary or reconstructive operation. We analyzed using 16S ribosomal RNA gene sequencing compared results among patient groups with POP, pessary, postoperation. also measured 10 inflammation-related cytokines swab...
Acute myeloid leukemia with myelodysplasia-related changes (AML-MRC) includes heterogeneous conditions such as previous history and specific cytogenetic morphological properties. In this study, we analyze genetic aberrations using an RNA-based next-generation sequencing (NGS) panel assay in 45 patients AML-MRC detect 4 gene fusions of KMT2A-SEPT9, KMT2A-ELL, NUP98-NSD1, RUNX1-USP42 81 somatic mutations. Overall, all had comprising not only changes, but also We demonstrated several...
Abstract Early T‐cell precursor acute lymphoblastic leukemia (ETP‐ALL) is a recently identified high‐risk subgroup of ALL in children. However, there have been conflicting reports and limited data reported adult patients. We retrospectively analyzed the cytogenetic molecular characteristics long‐term survival outcomes patients with ETP‐ALL versus non‐ETP‐ALL. 58 (median age, 35 years [range, 18–76 years]) newly diagnosed who received uniform remission induction consolidation chemotherapy...
Cytomegalovirus (CMV) infection is a serious complication in hematopoietic cell transplantation (HCT) recipients. Drug-resistant strains make it more challenging to treat CMV infection. This study aimed identify variants associated with drug resistance HCT recipients and assess their clinical significance. A total of 123 patients refractory DNAemia out 2271 at the Catholic Hematology Hospital between April 2016 November 2021 were analyzed, which accounted for 8.6% 1428 who received...
Rapid and accurate detection of carbapenemase-producing Enterobacteriaceae (CPE) is critical for appropriate treatment infection control. We compared a rapid fluorogenic assay using carbapenem-based probe with other phenotypic assays: modified carbapenem inactivation method (mCIM), Carba NP test (CNP), carbapenemase inhibition (CIT).
Granulocyte transfusion (GTx) is performed as a supportive therapy in severe neutropenic patients caused by various conditions. The study aimed to analyze the hematologic parameters of donors, patients, and granulocyte concentrates predict successful GTx.This was 281 with their being collected through apheresis, 54 who had diseases. Complete blood cell counts donors pre- post-apheresis, concentrates, post-GTx were analyzed. Patients divided into two groups according survival at discharge...
The objective of this study was to characterize circulating tumor DNA (ctDNA) mutations in colorectal cancer (CRC) patients and evaluate their prognostic values during treatment. Forty-nine with CRC planned for operation were enrolled. A total 115 plasma samples collected pre-operation, post-operation, post-chemotherapy. ctDNA analysis performed using next-generation sequencing (NGS) including 14 genes. In 22 (44.9%) out 49 patients, at least one mutation (40 mutations) detected the initial...
Abstract Introduction We retrospectively analyzed data of recipients who underwent three consecutive therapeutic plasma exchanges (TPEs) before major ABO‐incompatible (ABOi) hematopoietic stem cell transplantation (HSCT) in our hospital from 2012 to 2017 and evaluated the efficacy TPE for successful ABOi HSCT. Materials methods investigated 29 with HSCT based on following: (1) requirement red blood (RBC) transfusion during 100 days, (2) erythrocyte engraftment by reticulocyte count at 3...
Atypical hemolytic uremic syndrome (aHUS) is a rare disease caused by the dysregulation of alternative pathway complement system, leading to microvascular damage.It type thrombotic microangiopathy (TMA) characterized non-immune anemia, thrombocytopenia, and renal impairment.Half patients with aHUS develop end-stage kidney (ESKD) [1].Genetic testing crucial for diagnosis aHUS, as variants in regulatory protein gene significantly Successful treatment recurrent atypical posttransplantation
Summary Inherited bone marrow failure syndromes (IBMFS) pose significant diagnostic challenges due to overlapping symptoms and variable expressivity, despite evolving genomic insights. The study aimed elucidate the landscape among 130 Korean patients with IBMFS. We conducted targeted next‐generation sequencing (NGS) clinical exome (CES) across cohort, complemented by whole genome (WGS) chromosomal microarray (CMA) in 12 47 cases, respectively, negative initial results. Notably, 50% ( n = 65)...
Summary Juvenile myelomonocytic leukaemia (JMML), a rare clonal haematopoietic disorder of childhood, is characterised as myelodysplastic/myeloproliferative neoplasm. Despite ground‐breaking genetic discoveries, JMML remains difficult to diagnose given its diverse clinical features and disease course. A total 24 patients with were diagnosed treated at single institution, their profiles association laboratory characteristics analysed. In all, 22 the received allogeneic stem cell...