A5051254003- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Mycology
- Antifungal resistance and susceptibility
- Viral gastroenteritis research and epidemiology
- Fungal Infections and Studies
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Digital Holography and Microscopy
- Respiratory viral infections research
- Cell Image Analysis Techniques
- Plant Pathogens and Fungal Diseases
- Peptidase Inhibition and Analysis
- SARS-CoV-2 detection and testing
- Infective Endocarditis Diagnosis and Management
- Microscopic Colitis
- Image Processing Techniques and Applications
- Autoimmune and Inflammatory Disorders Research
- Magnetism in coordination complexes
Seoul St. Mary's Hospital
2019-2025
Catholic University of Korea
2020-2025
Samsung Medical Center
2016-2020
Sungkyunkwan University
2009-2020
Jeonju University
2015
Korea University
2009-2011
Kangnam University
2004
The healthcare industry is in dire need of rapid microbial identification techniques for treating infections. Microbial infections are a major issue worldwide, as these widespread diseases often develop into deadly symptoms. While studies have shown that an early appropriate antibiotic treatment significantly reduces the mortality infection, this effective difficult to practice. main obstacle treatments long turnaround time routine identification, which includes time-consuming sample growth....
ObjectivesThe performance of the investigational-use-only version BioFire FilmArray Pneumonia Panel (FA-Pneumo), a high-order nested multiplex PCR, was evaluated for detection typical respiratory bacterial pathogens and antibiotic resistance genes in sputa endotracheal aspirate (ETA) specimens.MethodsThirty-one 69 ETA specimens were analyzed. The diagnostic FA-Pneumo assessed using routine microbiological methods as reference standard.ResultsOverall sensitivity specificity organism 98.5%...
We evaluated the GenoType NTM-DR (NTM-DR) line probe assay for identifying Mycobacterium avium complex (MAC) species and abscessus subspecies determining clarithromycin amikacin resistance. Thirty-eight reference strains 145 clinical isolates (58 MAC 87 M. isolates), including 54 clarithromycin- and/or amikacin-resistant strains, were involved.
Objective and Impact Statement. We propose a rapid accurate blood cell identification method exploiting deep learning label-free refractive index (RI) tomography. Our computational approach that fully utilizes tomographic information of bone marrow (BM) white (WBC) enables us to not only classify the cells with but also quantitatively study their morphological biochemical properties for hematology research. Introduction. Conventional methods examining cells, such as smear analysis by medical...
In Young Yoo, M.D., Ph.D., Joo An Kwon, M.T., Miran Lee, Seung-Hyun Jung, Jung Ok Kim, M.S., Sung Il Ha, and Yeon-Joon Park, Ph.D.. Ann Lab Med -0001;0:. https://doi.org/10.3343/alm.2023.0430
Microbial contamination of platelet concentrates (PC) remains a persistent challenge in transfusion medicine, necessitating robust preventive measures prior to product release. Differentially expressed gene (DEG) analysis microbe inoculated PC offers promising approach identifying potential biomarkers for detection. Within PC, each S. aureus (ATCC 29213) and epidermidis 12228) was 103 CFU/mL concentration. Total RNA extracted from the samples at predetermined time points (0-, 1-, 3-, 6-hours...
Four cyanide-linked Fe(III)-Mn(III) complexes were prepared by reacting Mn Schiff bases with a new molecular precursor (PPh(4))[Fe(qcq)(CN)(3)] [1; qcq = 8-(2-quinolinecarboxamido)quinoline anion]. They include dimeric molecule, [Fe(qcq)(CN)(3)][Mn(3-MeOsalen)(H(2)O)] x 2 H(2)O [2 H(2)O; 3-MeOsalen N,N'-ethylenebis(3-methoxysalicylideneiminato) dianion], and three 1D zigzag chains, [Fe(qcq)(CN)(3)][Mn(5-Clsalen)] 3 [3 MeOH; 5-Clsalen N,N'-ethylenebis(5-chlorosalicylideneiminato)...
As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated minimum inhibitory concentrations (MICs), using VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven isolates from 14 patients exhibiting MICs ≥8 μg/mL were examined by broth microdilution (BMD) test as well sequencing for mutations 23S rRNA gene or ribosomal proteins (L3, L4, and L22) presence...
Five Fe(III)Mn(III) bimetallic compounds [Fe(iqc)(CN)(3)][Mn(5-Xsalen)]·pMeOH·qMeCN·rH(2)O [Hiqc = N-(quinolin-8-yl)isoquinoline-1-carboxamide; salen N,N'-ethylenebis(salicylideneiminato) dianion; X H(2), F(3, 3a), Cl(4), Br(5)] were prepared by assembling a newly designed mer-Fe tricyanide (Ph(4)P)[Fe(iqc)(CN)(3)]·0.5H(2)O (1) and the respective Mn Schiff bases Mn(5-Xsalen)(+). Compounds 2-4 show linear chain structures in which trans-positioned cyanides of Fe precursor bridge neighbouring...
Infectious diarrhea is a global pediatric health concern; therefore, rapid and accurate detection of enteropathogens vital. We evaluated the BioFire® FilmArray® Gastrointestinal (GI) Panel with that comparator laboratory tests. Stool samples patients were prospectively collected tested. As method for bacteria, culture, conventional PCR diarrheagenic E. coli, Allplex GI-Bacteria(I) Assay For discrepancy analysis, BD MAX Enteric Bacterial was used. virus, Virus immunochromatography used...
ObjectivesThis study aimed to identify the cause of false-positive serum Aspergillus antigen galactomannan (GM) results in our centre.MethodsWe performed a case-control aiming elucidate factors associated with GM results. Independent risk for were evaluated through multivariable regression analysis. An interrupted time series analysis was used evaluate effectiveness an intervention removing identified factors.ResultsAmong 568 patients tested, positive 130 whom 97 had (cases). These compared...
Data on the frequency of gyrA and gyrB mutations in fluoroquinolone-resistant isolates Mycobacterium avium complex (MAC) abscessus (MABC) are limited. In our analysis, we did not find any resistance-associated or 105 MAC MABC clinical isolates, including 72 moxifloxacin-resistant isolates. Our findings suggest that mechanisms other than contribute to moxifloxacin resistance these organisms.
To evaluate the performance of a rapid antimicrobial susceptibility testing (AST) platform based on microfluidic chip technology, QMAC-dRAST, which enables AST from colony isolates or positive blood culture broth (PBCB), and to compare QMAC-dRAST for staphylococci enterococci with that VITEK-2 system reference microdilution (BMD). A total 110 staphylococcal enterococcal cultures were included. was performed directly using PBCB. Thereafter, derived subculture PBCB used BMD. The overall...
The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages manual IIF assays have highlighted need automation and standardization fluorescent ANA (FANA) testing. We evaluated performance EUROPattern Suite (Euroimmun AG, Germany), an automated FANA image analyzer, regard to detection pattern recognition compared conventional interpretation using fluorescence...
As 16S ribosomal RNA (rRNA)-targeted sequencing can detect DNA from non-viable bacteria, it be used to identify pathogens clinical samples even in patients pretreated with antibiotics. We compared the results of rRNA-targeted and culture for identifying bacterial species normally sterile body fluid (NSBF): cerebrospinal, pericardial, peritoneal pleural fluids. Over a 10-year period, total 312 NSBF were evaluated simultaneously using culture. Results concordant 287/312 (92.0%) samples,...
We evaluated the efficacy of intermittent azithromycin and ethambutol therapy for noncavitary Mycobacterium avium complex pulmonary disease (MAC-PD). Twenty-nine (76%) 38 patients achieved sputum culture conversion after 12 months treatment, smear positivity was an independent factor failure to achieve (adjusted odds ratio, 26.7; 95% confidence interval, 2.1 339.9; P = 0.011). Intermittent may be optional treatment regimen MAC-PD.
Conventional methods for etiologic diagnoses of acute gastroenteritis (AGE) are time consuming and have low positive yield leading to limited clinical value. This study aimed investigate quality improvements in patient management, antibiotic stewardship, in-hospital infection transmission prevention using BioFire® FilmArray® Gastrointestinal Panel (GI Panel) children with diarrhea. was a prospective recruiting < 19 years old new onset diarrhea during the period, matched historical cohort...
Minimal residual disease (MRD) is an important prognostic factor for evaluating a deeper treatment response in patients with multiple myeloma (MM). We evaluated the clinical utility of next-generation flow (NGF)-based MRD assessment heterogeneous MM patient population.Patients suspected morphological remission after or during were prospectively enrolled. In total, 108 bone marrow samples from 90 analyzed using NGF-based according to EuroFlow protocol, and progression-free survival (PFS) was...
Abstract Background Drug-resistant tuberculosis (TB) is a major threat to global public health. Whole-genome sequencing (WGS) useful tool for species identification and drug resistance prediction, many clinical laboratories are transitioning WGS as routine diagnostic tool. However, user-friendly high-confidence automated bioinformatics tools needed rapidly identify M. complex (MTBC) non-tuberculous mycobacteria (NTM), detect resistance, further guide treatment options. Results We developed...
Recently, the American Thoracic Society/Infectious Diseases Society of America/Centers for Disease Control and Prevention advised against performing interferon-γ-release assay (IGRA) test individuals with a low risk TB, also recommended retesting low-risk an initial positive IGRA result. However, to evaluate both sensitivity specificity available tests, we compared performance Standard E TB-Feron (TBF) QuantiFERON-TB Gold Plus (QFT-Plus) assays in healthcare workers (HCWs) tuberculosis (TB)...
We evaluated the performance of MicroIDSys Elite system, a newly developed matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry system for identification mycobacteria directly from positive MGIT liquid cultures.Analytical specificity was with 63 reference strains grown in growth indicator tube media. Prospective evaluation conducted primary cultures sputum samples mycobacteria, and results were compared to multigenerational sequencing as method. Liquid...
The GENEDIA MTB/NTM Detection Kit (GENEDIA MTB/NTM; Green Cross Medical Science Corp., Chungbuk, Korea) is a multiplex real-time PCR assay used for differential identification of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM). While the importance recognized, there limited data on performance to date. A total 687 consecutive sputum specimens were cultured analyzed with MTB assays. Nineteen (2.8%) MTBC-positive, 69 (10.0%) NTM-positive based mycobacterial...