A5009258022- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 detection and testing
- Hemoglobinopathies and Related Disorders
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Chronic Lymphocytic Leukemia Research
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Nail Diseases and Treatments
- Clostridium difficile and Clostridium perfringens research
- Hepatitis C virus research
- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- Infections and bacterial resistance
- Fetal and Pediatric Neurological Disorders
- Genomic variations and chromosomal abnormalities
- Antimicrobial Resistance in Staphylococcus
- Platelet Disorders and Treatments
- Phagocytosis and Immune Regulation
- Blood transfusion and management
Samsung Medical Center
2020-2024
Sungkyunkwan University
2020-2024
Seoul National University
2017-2019
Seoul National University Hospital
2018-2019
Seoul National University Bundang Hospital
2019
High concentration of mold spores in inhaled air is an important risk factor for invasive infections. COVID‐19 (coronavirus disease 2019)‐associated pulmonary aspergillosis (CAPA) a serious complication severe COVID‐19. To investigate the distribution negative‐pressure isolation wards and its potential association with CAPA incidence, we conducted microbiological sampling retrospectively analyzed cases tertiary care hospital Korea during pandemic. Air was January 2022 at multiple sites four...
Rh hemolytic disease of the fetus and newborn is a potential risk for D-negative mothers who produce anti-D during pregnancy, which can lead to morbidity mortality in subsequent pregnancies.To prevent this disease, Rho(D) immune globulin (RhIG) generally administered without at 28 weeks gestation shortly after delivery.However, current guidelines suggest that pregnant with molecularly defined weak D types 1, 2, 3, 4.0, 4.1 do not need RhIG as they are unlikely alloanti-D when exposed fetuses...
We investigated the evolution of fluconazole resistance mechanisms and clonal types Candida parapsilosis isolates from a tertiary care hospital in South Korea. A total 45 clinical isolates, including 42 collected between 2017 2021 3 2012 2013, were subjected to antifungal susceptibility testing, sequencing genes (ERG11, CDR1, TAC1, MRR1), microsatellite typing. Twenty-two carried Y132F (n = 21; MIC 2 >256 mg/L) or Y132F+R398I 1; 64 ERG11 four harbored N1132D CDR1 (fluconazole 16 mg/L). All...
Carbapenem-resistant Acinetobacter baumannii (CRAB) outbreaks in hospital settings challenge the treatment of patients and infection control. Understanding relatedness clinical isolates is important distinguishing outbreak from sporadic cases. This study investigated 11 CRAB a by whole-genome sequencing (WGS), utilizing various bioinformatics tools for analysis. The results multilocus sequence typing (MLST), single nucleotide polymorphism (SNP) analysis, phylogenetic tree analysis WGS...
ABSTRACT Background Rapid antimicrobial susceptibility testing (AST) for bloodstream infections (BSIs) facilitates the optimization of therapy, preventing resistance and improving patient outcomes. QMAC‐dRAST (QuantaMatrix Inc., Korea) is a rapid AST platform based on microfluidic chip technology that performs directly using positive blood culture broth (PBCB). This study evaluated performance Gram‐negative bacteria PBCB subcultured colony isolates, comparing it with VITEK 2 (bioMérieux,...
This study compared the performance of two commercial molecular assays, STANDARD M10
The aim of this study was to compare the performance newly developed SMG HHV-6 Q Real-Time PCR Kit (SMG assay) with RealStar (RealStar assay). analytical sensitivity and specificity, linearity, precision assay were evaluated. clinical assessed compared that using 207 specimens (HHV-6A positive,
With the introduction of ceftazidime-avibactam worldwide, antimicrobial activity new β-lactam/β-lactamase inhibitors (BL/BLIs) needs to be investigated. From January 2020 June 2023, Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales were collected. a broth microdilution test BL/BLIs, cross-activity with nine combinations BLs and BLIs dose-escalation titration for non-susceptible isolates conducted investigate inhibitory activities BLIs. A total 188 was collected most...
DEL, the weakest D variant, is mistyped as D-negative by routine serological assays. Transfusion of red blood cells expressing DEL phenotype has potential to elicit anti-D alloimmunization in recipients. The goal this study was recommend typing strategies for serologically Asian donors.RhCE phenotyping and adsorption-elution test were performed on 674 samples. RHD genotyping using real-time polymerase chain reaction melting curve analysis also undertaken identify alleles. Costs turnaround...
Background ALX148, a novel CD47 blocking agent, is in clinical development for the treatment of advanced solid tumors and lymphoma. Because highly expressed on red blood cells (RBCs), its therapeutic blockade can potentially interfere with pretransfusion compatibility testing. This study describes interference ALX148 testing evaluates methods used mitigating such interference. Study Design Methods Routine serologic tests were performed six samples from four patients treated ALX148. Antibody...
The World Health Organization recently lowered the rifampin (RIF) critical concentration (CC) for drug-susceptibility testing (DST) of Mycobacterium tuberculosis complex (MTBC) using mycobacterial growth indicator tube (MGIT) 960 system. Here, we evaluated diagnostic performance MGIT system with revised CC determining MTBC RIF resistance 303 clinical isolates, including 122 isolates rpoB mutations, which 32 had single borderline-resistance and 181 wild-type isolates. phenotypic was...
We evaluated the performance of MicroIDSys Elite system, a newly developed matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry system for identification mycobacteria directly from positive MGIT liquid cultures.Analytical specificity was with 63 reference strains grown in growth indicator tube media. Prospective evaluation conducted primary cultures sputum samples mycobacteria, and results were compared to multigenerational sequencing as method. Liquid...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and influenza viruses may pose enormous challenges to our healthcare system. We evaluated the performance of PowerChek SARS-CoV-2, Influenza A & B Multiplex Real-time PCR Kit (PowerChek; Kogene Biotech, Seoul, Korea) in comparison with BioFire Respiratory Panels 2 2.1 (RP2 RP2.1; bioMérieux, Marcy l'Étoile, France), using 147 nasopharyngeal swabs. The limit detection (LOD) assay was determined A, RNA standards. LOD values for...
We believe that this is the first study describe microbiological and molecular characteristics of bloodstream isolates C. parapsilosis in Korea exhibiting two phenotypes (sinking floating). An important aspect our findings sinking phenotype was observed predominantly harboring a Y132F substitution ERG11 gene (92.9%), fluconazole-nonsusceptible (FNS) (86.7%), clonal BSI (74.4%) .
ABSTRACT In October 2022, we experienced a significant increase in samples showing high galactomannan (GM) indexes ranging from 6.22 to 10.58, as determined by the Platelia Aspergillus antigen immunoassay, also known GM test. After reviewing medical records of nine antigenemia cases that did not show evidence invasive aspergillosis, found these patients had received total parenteral nutrition (TPN) products same manufacturer, whose supplier glucose component recently changed. The TPN...
Next-generation sequencing (NGS) technology has been recently introduced into blood group genotyping; however, there are few studies using NGS-based genotyping in real-world clinical settings. In this study, we applied various immunohaematology cases encountered routine practice.This study included 4 cases: ABO subgroup, chimerism, antibody to a high-frequency antigen (HFA), and anti-CD47 interference. We designed hybridization capture-based NGS panel targeting 39 group-related genes it the...
The T-SPOT.TB can be read by an ELISPOT plate imager as alternative to a labor-intensive and time-consuming manual reading, but its accuracy has not been sufficiently discussed date.1,423 test results obtained from reading using microscope were compared. agreement of qualitative was assessed Cohen's kappa coefficient. relationship spot counts studied Bland-Altman analysis.The overall percent the 95.43% with coefficient 0.91. Positive maximum net count 8 borderline showed relatively high...
Several studies on Chinese and Japanese populations have revealed that a substantial proportion of weak B subgroups are caused by variants in the major regulatory regions ABO, proximal promoter, CCAAT-binding factor/NF-Y binding site +5.8-kb site. We performed molecular analyses these Koreans with phenotypes.This study included 16 samples phenotypes (4 B3 , 1 Bw 5 A1 6 ) harbouring no subgroup-causing ABO exons 7. These were subjected to sequencing analysis 1-5 ABO.Of samples, 14 found carry...
<b><i>Introduction:</i></b> Evorpacept is a CD47-blocking agent currently being developed for the treatment of various cancers. Interference by evorpacept in pretransfusion compatibility testing has been reported at limited plasma concentrations. Although mitigation strategies have proposed, none are practical. This vitro study assessed evorpacept-induced interference extended concentrations and investigated capability novel agent, Evo-NR....
In the ongoing global fight against coronavirus disease 2019 (COVID-19), sample preparation process for real-time reverse transcription polymerase chain reaction (rRT-PCR) faces challenges due to time-consuming steps, labor-intensive procedures, contamination risks, resource demands, and environmental implications. However, optimized strategies have been poorly investigated, combination of RNase inhibitors Proteinase K has rarely considered. Hence, we investigated combinations several...
Facklamia hominis is a facultative anaerobic Gram-positive coccus generally displaying weak alpha-hemolysis and negativity for catalase oxidase. species are part of the normal flora female genitourinary tract have been reported in invasive diseases such as meningitis infective endocarditis, albeit rarely. A 67 year-old-man presented to hospital with tender, erythematous epidermal cyst on right side his upper back. Simple excision was performed pus taken sterile swab culture, yielding no...